A7Z4J2 (A7Z4J2_BACA2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Peptide deformylase 2 HAMAP MF_00163
Short name=PDF 2 HAMAP MF_00163
EC=3.5.1.88 HAMAP MF_00163

Alternative name(s):
Polypeptide deformylase 2 HAMAP MF_00163

Gene names

Name:	defA EMBL ABS73918.1
Synonyms:	def2 HAMAP MF_00163
Ordered Locus Names:	RBAM_015550

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	160 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP MF_00163
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163 SAAS SAAS000181
Cofactor	Binds 1 Fe2+ ion By similarity. HAMAP MF_00163
Sequence similarities	Belongs to the polypeptide deformylase family. HAMAP MF_00163 RuleBase RU003335

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00163 SAAS SAAS000181
Ligand	Iron HAMAP MF_00163 Metal-binding HAMAP MF_00163 SAAS SAAS000181
Molecular function	Hydrolase SAAS SAAS000181 HAMAP MF_00163 EMBL ABS73918.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: InterPro peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	133	1	By similarity HAMAP MF_00163
	Metal binding	90	1	Iron By similarity HAMAP MF_00163
	Metal binding	132	1	Iron By similarity HAMAP MF_00163
	Metal binding	136	1	Iron By similarity HAMAP MF_00163

Sequences

	Sequence		Length	Mass (Da)
	A7Z4J2 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 9427246EF5E6D194	FASTA	160	17,634
	10 20 30 40 50 60 MAVKKIVTHP AEVLETPADE VTVFDKKLKK LLDDMYDTML EMDGVGLAAP QIGILKRAAV 70 80 90 100 110 120 VDIGEESGRI DLVNPVILEK SGEQTGVEGC LSFPGVYGDV TRPDYVKVKA YDRKGKPFIL 130 140 150 160 EAEGFLARAV QHEMDHLDGI LFTSKITAYY TEEELAEMEG

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS73918.1.
RefSeq	YP_001421149.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z4J2.
SMR	A7Z4J2. Positions 1-150.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z4J2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000007162; EBBACP00000007040; EBBACG00000007154.
GeneID	5460549.
GenomeReviews	Gene locus RBAM_015550 in contig CP000560_GR.
KEGG	bay:RBAM_015550.
PATRIC	18748174. VBIBacAmy31356_1563.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0242.
GeneTree	EBGT00050000001175.
HOGENOM	HBG665227.
OMA	VWEPIGE.
ProtClustDB	PRK12846.
Family and domain databases
HAMAP	MF_00163. Pep_deformylase. [Tree]
InterPro	IPR000181. Fmet_deformylase. IPR023635. Peptide_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
KO	K01462.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
SUPFAM	SSF56420. Fmet_deformylase. 1 hit.
TIGRFAMs	TIGR00079. Pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A7Z4J2_BACA2

Accession

Primary (citable) accession number: A7Z4J2

Entry history

Integrated into UniProtKB/TrEMBL:	October 23, 2007
Last sequence update:	October 23, 2007
Last modified:	December 14, 2011
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)