Reviewed, UniProtKB/Swiss-Prot A7Z4H4 (PYRD_BACA2)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	RBAM_015370

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O2 = orotate + H2O2. HAMAP MF_00224
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6. HAMAP MF_00224
Subunit structure	Heterotetramer of 2 pyrK and 2 pyrD subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cytoplasm
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Dihydroorotate dehydrogenase HAMAP MF_00224		PRO_1000024121
Sites
	Active site	128	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z4H4-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: DFCCFF8D08521A00	FASTA	311	33,022
	10 20 30 40 50 60 MLEVNLPGLQ LKNPIIPASG CFGFGKEYAG FYDLSCLGGI MIKATTNEAR FGNPTPRVAE 70 80 90 100 110 120 TSAGMLNAIG LQNPGLEHVL NHELPWLEQF DTPIIANVAG SQVDDYKEVA EHISQAPNVH 130 140 150 160 170 180 ALELNISCPN VKTGGIAFGT DPVMAAELTK AVKEVSAVPV YVKLSPNVAN ITEIAKAIEA 190 200 210 220 230 240 AGADGLTMIN TLIGMRLDLK TGRPILANKT GGLSGPAIKP VAIRMVYEVS QAVHIPIIGM 250 260 270 280 290 300 GGVQTAEDAL EFLLAGASAV AVGTANFVNP FVCPEIIEQL PRVLRQYGFQ SVSECIGRSW 310 KHEKQPAHHR A

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000560 Genomic DNA. Translation: ABS73900.1.
RefSeq	YP_001421131.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5461856.
GenomeReviews	Gene locus RBAM_015370 in contig CP000560_GR.
KEGG	bay:RBAM_015370.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A7Z4H4. NSIGLQN.
Family and domain databases
HAMAP	MF_00224. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005720. Dihydroorotate_DH_1_core. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01037. pyrD_sub1_fam. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_BACA2

Accession

Primary (citable) accession number: A7Z4H4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	June 16, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)