Reviewed, UniProtKB/Swiss-Prot A7Z4G5 (LSPA_BACA2)

Names and origin

Protein names

Recommended name:
    Lipoprotein signal peptidase
    EC=3.4.23.36
Alternative name(s):
    Prolipoprotein signal peptidase
    Signal peptidase II
      Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	RBAM_015280

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 153	153	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000190787
Regions
	Transmembrane	52 – 72	21	Potential
	Transmembrane	81 – 101	21	Potential
	Transmembrane	124 – 144	21	Potential
Sites
	Active site	102	1	By similarity
	Active site	129	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z4G5-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 8ECE4AA25D3F8250	FASTA	153	17,243
	10 20 30 40 50 60 MLYYLIALFI IIADQLTKWL VVSHMELGQS IPVIDQVLYI TSHRNTGAAW GILAGQMWFF 70 80 90 100 110 120 YVITIAVIIG IVYYIQRYAK GQMLLGISLG LMLGGAAGNF IDRAARQEVV DFIHVIIVDY 130 140 150 HYPIFNIADS SLCVGVILLF IHMLFDSGKK KEQ

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS73891.1.
RefSeq	YP_001421122.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z4G5.
Protein family/group databases
MEROPS	A08.001.
Genome annotation databases
GeneID	5462523.
GenomeReviews	Gene locus RBAM_015280 in contig CP000560_GR.
KEGG	bay:RBAM_015280.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0597.
HOGENOM	HBG724422.
OMA	DQASKRW.
Family and domain databases
HAMAP	MF_00161. LspA. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. lspA. 1 hit.
PROSITE	PS00855. SPASE_II. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_BACA2

Accession

Primary (citable) accession number: A7Z4G5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 23, 2007
Last modified:	February 9, 2010
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)