A7Z4E3 (MURD_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	RBAM_015060

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_1000056872
Regions
	Nucleotide binding	120 – 126	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A7Z4E3 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: C2AA5E4EE1765896	FASTA	451	49,443
	10 20 30 40 50 60 MENELFLQKQ NFLVLGLAKS GYAAASILHE KGINVVVNDQ KAFEENEPAQ RLAERGIEVI 70 80 90 100 110 120 CGEHPTSLFD QHHITILIKN PGIPYENIMV EEAQRRGIPV WTEIELAYYI TNAKFIGITG 130 140 150 160 170 180 SNGKTTTTTL IYEMLKADSI KTLIAGNIGT VASEVAYHAD GDEWIVTELS SFQLMGTHAF 190 200 210 220 230 240 RPEIGLILNV FDAHLDYHHS RENYEKAKQN VYLHQLESDT AIVNQSDETV VRLAESGKAG 250 260 270 280 290 300 TVPFSVHQEL SSGAFIKDGM LMFGDEAILP VDDIVLPGAH NLENILAAVA AAKTAGASNK 310 320 330 340 350 360 AIQKVLTSFT GVKHRLQYVT AIQNRKFYND SKATNILATS KALSAFKAPV ILLAGGLDRG 370 380 390 400 410 420 NGFDDLKPYM DNVKAVLTFG QTAPKIEKLG NELGIQHVKR VDNVEQAVSA AFALSNEGDV 430 440 450 ILLSPACASW DQFKTFEERG DMFIDAVHML K

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS73869.1.
RefSeq	YP_001421100.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z4E3.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z4E3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000007730; EBBACP00000007608; EBBACG00000007722.
GeneID	5461796.
GenomeReviews	Gene locus RBAM_015060 in contig CP000560_GR.
KEGG	bay:RBAM_015060.
PATRIC	18748070. VBIBacAmy31356_1511.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0771.
GeneTree	EBGT00070000032130.
HOGENOM	HBG750024.
OMA	ACASWDM.
ProtClustDB	PRK02472.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_015060-MONOMER.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BACA2

Accession

Primary (citable) accession number: A7Z4E3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program