Reviewed, UniProtKB/Swiss-Prot A7Z4E1 (MURE_BACA2)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	RBAM_015040

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_1000012336
Regions
	Nucleotide binding	109 – 115	7	ATP Potential
	Region	151 – 152	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	408 – 411	4	Meso-diaminopimelate binding By similarity
	Motif	408 – 411	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	31	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	150	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	178	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	186	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	384	1	Meso-diaminopimelate By similarity
	Binding site	458	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	462	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	218	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z4E1-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: DF23F229249AF110	FASTA	490	53,948
	10 20 30 40 50 60 MKLTKLLTYL MAEPVQNDFH DPDITSIEMD SREVRKGSLF VCIKGYTVDG HDFAQKAAEN 70 80 90 100 110 120 GAAAIVAEKE LDVDVPVIIV RRSQRALSVL SDAFYGQPTK QLQLIGITGT NGKTSTTHMV 130 140 150 160 170 180 DEIFKKAGRQ TGLIGTMYIK IGDETFPVKN TTPESVTLQK TFKKMNDEHV DTAIMEVSSH 190 200 210 220 230 240 ALSLGRVHGC DYDIAVFTNL TQDHLDYHKT MEDYRQAKSL LFSQLGGSFN HEKPKRAVLN 250 260 270 280 290 300 ADDKASAYFE KVTAAHILTY GIENDADVMA KQIEISAQGT SFELVTPKGT KQITVSLVGR 310 320 330 340 350 360 FNVYNVLAAA ATGIAAGLPF DTITSALEEL QGVRGRFELV NHNQPFPVVV DYAHTPDSLE 370 380 390 400 410 420 NVLNTCKDMT EGKLFVVVGC GGDRDKTKRP KMAEIAVRIA DEPIFTSDNP RSEDPLAILR 430 440 450 460 470 480 DMERGVEGTY YHSIANREQA IFFAIANAKK GDVVLIAGKG HETYQQIGNE TFDFDDAEVA 490 GRAIVELNKK

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000560 Genomic DNA. Translation: ABS73867.1.
RefSeq	YP_001421098.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z4E1.
Genome annotation databases
GeneID	5461794.
GenomeReviews	Gene locus RBAM_015040 in contig CP000560_GR.
KEGG	bay:RBAM_015040.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FPVIVDY.
Family and domain databases
HAMAP	MF_00208. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_BACA2

Accession

Primary (citable) accession number: A7Z4E1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)