Reviewed, UniProtKB/Swiss-Prot A7Z3U1 (METE_BACA2)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	RBAM_013040

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 762	762	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017219
Sites
	Metal binding	645	1	Zinc By similarity
	Metal binding	647	1	Zinc By similarity
	Metal binding	730	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z3U1-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 455F1426B1CFAD37	FASTA	762	86,581
	10 20 30 40 50 60 MTTIKTSNLG FPRIGLNREW KKTLEAYWKG NTDKETFLKE LDGLFLSAVK TQLDQHIDIV 70 80 90 100 110 120 PVSDFTHYDH VLDTAVSFNW IPKRFRSITD PVDTYFAIAR GVKDAVSSEM TKWFNTNYHY 130 140 150 160 170 180 IVPEYDESIE FRLTRNKQLD DYRRIKEEFG AETKPVIVGP YTFVTLAKGY EESEAKAIQK 190 200 210 220 230 240 RLVPLYVQLL KELEAEGVKW VQIDEPALVT ASSEDVRAAK ELYESITKEL SGLNVLLQTY 250 260 270 280 290 300 FDSVDAYEEL VSYPVQGIGL DFVHDKGRNL DQLKKHGFPK DKVLAAGVID GRNIWKIDVE 310 320 330 340 350 360 ERLDAALDIL SHADVEELWI QPSSSLLHVP VAKHPDEHLE KDLLNGLSYA KEKLAELGVL 370 380 390 400 410 420 KEGLLSGKAA VSEQIEESKA ALKALKAFAT GANSAQKEEL NQLTEKDFSR PASFEERLAL 430 440 450 460 470 480 QNESLGLPLL PTTTIGSFPQ SAEVRSARQK WRKKDWTDEQ YQAFINAETK RWVDIQEEIG 490 500 510 520 530 540 LDVLVHGEFE RTDMVEYFGE KLAGFAFTKY AWVQSYGSRC VRPPVIFGDV EFIEPMTVKD 550 560 570 580 590 600 TVYAQSLTEK HMKGMLTGPV TILNWSFPRV DISRKEIAFQ IGLALRKEVK ALEDAGIQII 610 620 630 640 650 660 QVDEPALREG LPLKTQDWDE YLTWAAEAFR LTTSSVQNET QIHTHMCYSN FEDIVDTIND 670 680 690 700 710 720 LDADVITIEH SRSHGGFLEY LKEHPYVKGL GLGVYDIHSP RVPATEEIYQ IIDDALEVCP 730 740 750 760 TDRFWVNPDC GLKTRQQEET VAALKNMVDA AKQARKKQAQ LV

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000560 Genomic DNA. Translation: ABS73667.1.
RefSeq	YP_001420898.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z3U1.
Genome annotation databases
GeneID	5461876.
GenomeReviews	Gene locus RBAM_013040 in contig CP000560_GR.
KEGG	bay:RBAM_013040.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CSLLHTP.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProDom	PD004692. Methionine_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BACA2

Accession

Primary (citable) accession number: A7Z3U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)