A7Z3F2 (PRPE_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical]
EC=3.6.1.17

Alternative name(s):
Ap4A hydrolase
Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
Short name=Diadenosine tetraphosphatase

Gene names

Name:	prpE
Ordered Locus Names:	RBAM_011640

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Asymmetrically hydrolyzes Ap4p to yield AMP and ATP By similarity. HAMAP MF_01443
Catalytic activity	P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP. HAMAP MF_01443
Cofactor	Nickel By similarity. HAMAP MF_01443
Sequence similarities	Belongs to the prpE family.

Ontologies

Keywords
Ligand	GTP-binding Nickel Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Inferred from electronic annotation. Source: EC nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] HAMAP MF_01443		PRO_1000024421

Sequences

	Sequence		Length	Mass (Da)
	A7Z3F2 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 6E795F074F8086C8	FASTA	249	28,124
	10 20 30 40 50 60 MAYDIISDIH GCYDEMIALM QKLGYEVKDG TPVHSEDRTL VFAGDLTDRG PKSVEVMRFV 70 80 90 100 110 120 AKAYEKGAVR YVPGNHCNKL YRYLKGNPVK VMHGLETTAA ELKELSPADR TEVSRQFIRL 130 140 150 160 170 180 YESAPLYDIL HNGDLVVAHA GIKADDIGKY SRKVKEFVLY GDITGETHPD GRPVRRDWAA 190 200 210 220 230 240 AYEGKAWIVY GHTPVKRPRI VNRTINIDTG CVFGNQLTGF RFPEHETVSV ESSMPYDASR FREDHGRQA

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS73528.1.
RefSeq	YP_001420759.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z3F2.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z3F2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000007283; EBBACP00000007161; EBBACG00000007275.
GeneID	5459893.
GenomeReviews	Gene locus RBAM_011640 in contig CP000560_GR.
KEGG	bay:RBAM_011640.
PATRIC	18747328. VBIBacAmy31356_1142.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0639.
GeneTree	EBGT00050000002430.
HOGENOM	HBG724457.
OMA	YVPGNHC.
ProtClustDB	PRK13625.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_011640-MONOMER.
Family and domain databases
HAMAP	MF_01443. PrpE. [Tree]
InterPro	IPR023937. Bis(5'-nucleosyl)-tetraP_PrpE. IPR004843. Metallo_PEstase_dom. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRPE_BACA2

Accession

Primary (citable) accession number: A7Z3F2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program