Reviewed, UniProtKB/Swiss-Prot A7Z3A9 (ARGB_BACA2)

Names and origin

Protein names

Recommended name:
    Acetylglutamate kinase
    EC=2.7.2.8
Alternative name(s):
    NAG kinase
      Short name=AGK
    N-acetyl-L-glutamate 5-phosphotransferase

Gene names

Name:	argB
Ordered Locus Names:	RBAM_011210

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP acetylglutamate kinase activity Inferred from electronic annotation. Source: HAMAP glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Acetylglutamate kinase HAMAP MF_00082		PRO_1000010483
Regions
	Region	41 – 42	2	Substrate binding By similarity
Sites
	Binding site	63	1	Substrate By similarity
	Binding site	156	1	Substrate By similarity
	Site	8	1	Transition state stabilizer By similarity
	Site	215	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z3A9-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 97E9876A4D3DBCE0	FASTA	258	27,555
	10 20 30 40 50 60 MKKTIVFKCG GSVIRELSAD FFQNVKELMQ SGWNIAVVHG GGPDISKMLK TLQIETEFVD 70 80 90 100 110 120 GQRKTTKPVL ETAEMVLSGT INKFFVTELA KNGLKAAGIS GKDGGLLQAS YLNQDKYGEV 130 140 150 160 170 180 GEIQKTDPSI IHALMKEGII PVIAPLSMTS DFETLNVNAD AAASAVASAL HADKLLFVTD 190 200 210 220 230 240 VEGIMDGESR LDTLTPQEIQ ALIDDGVISG GMIPKVNSAL SALSEDVEEV MIVNGKGAFF 250 TDQAFQGTKI VKEKESVS

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000560 Genomic DNA. Translation: ABS73485.1.
RefSeq	YP_001420716.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z3A9.
Genome annotation databases
GeneID	5462907.
GenomeReviews	Gene locus RBAM_011210 in contig CP000560_GR.
KEGG	bay:RBAM_011210.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FVIKYGG.
Family and domain databases
HAMAP	MF_00082. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR011148. GlcNAc_kinase. IPR001057. Glu_5kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
TIGRFAMs	TIGR00761. argB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_BACA2

Accession

Primary (citable) accession number: A7Z3A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)