A7Z2U3 (GLPK_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	RBAM_009550

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Glycerol kinase HAMAP MF_00186		PRO_1000020697
Regions
	Nucleotide binding	410 – 414	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	266	1	ATP By similarity
	Binding site	309	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z2U3 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 59D714BE45D13463	FASTA	496	54,846
	10 20 30 40 50 60 METYILSIDQ GTTSSRAILF NKEGKIVHSA QKEFTQHFPQ PGWVEHNANE IWGSVLAVIA 70 80 90 100 110 120 TVISESGISA DQIAGIGITN QRETTVVWDK DTGKPVYNAI VWQSRQTSGI CEELREKGYN 130 140 150 160 170 180 DTFREKTGLL IDPYFSGTKV KWILDNVEGA REKAENGNLL FGTIDSWLIW KMSGGAAHVT 190 200 210 220 230 240 DYSNASRTLM FNIHELKWDD ELLDILGVPK SMLPEVKPSS HVYAKTVDYH FFGKNIPIAG 250 260 270 280 290 300 AAGDQQSALF GQACFEEGMG KNTYGTGCFM LMNTGEKAIT SDHGLLTTIA WGLDGKVEYA 310 320 330 340 350 360 LEGSIFVAGS AIQWLRDGMR MFQDSKESET YAERVDSADG VYVVPAFVGL GTPYWDSDVR 370 380 390 400 410 420 GSVFGLTRGT TKEHFIRATL ESLAYQAKDV LDAMEADSGI SLKTLRVDGG AVKNNFLMQF 430 440 450 460 470 480 QSDLLDVPVE RPEINETTAL GAAYLAGIAV GYWKDSTEIA DQWNLDKQFK PEMEDEKRDE 490 LYGGWKKAVN AAMAFK

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS73319.1.
RefSeq	YP_001420550.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z2U3.
SMR	A7Z2U3. Positions 4-489.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z2U3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000006366; EBBACP00000006244; EBBACG00000006358.
GeneID	5460496.
GenomeReviews	Gene locus RBAM_009550 in contig CP000560_GR.
KEGG	bay:RBAM_009550.
PATRIC	18746887. VBIBacAmy31356_0922.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0554.
GeneTree	EBGT00050000001374.
HOGENOM	HBG511469.
OMA	ALYGQLC.
ProtClustDB	PRK00047.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_009550-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_BACA2

Accession

Primary (citable) accession number: A7Z2U3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program