A7Z2G6 (AROD_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase
Short name=3-dehydroquinase
EC=4.2.1.10

Alternative name(s):
Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	RBAM_008080

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214
Subunit structure	Homodimer By similarity. HAMAP MF_00214
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	3-dehydroquinate dehydratase HAMAP MF_00214		PRO_1000043160
Sites
	Active site	143	1	Proton acceptor By similarity
	Active site	170	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z2G6 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 3078B023F7F3B849	FASTA	253	28,085
	10 20 30 40 50 60 MQSITIRNTV IGEGLPKIIV PLMAAGEKEL LEEIEAVNRL RPDIIEWRAD VYEHVDSLDA 70 80 90 100 110 120 VKDMLEMLRK AAGATPLLFT FRTHKEGGNK VIDDRFYIEL LKTAIETKHI DLADVELFTG 130 140 150 160 170 180 EAEVKLIVKT AEDNGVYVVM SNHDFHQTPK KEEIISRLRN MQAYGAHIPK IAVMPQSTED 190 200 210 220 230 240 VFVLLDATHT MKTQYADRPI ITMSMAGTGL ISRLAGEVFG SACTFGAGKE ASAPGQIPVE 250 ELRSVLSILN KHM

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS73192.1.
RefSeq	YP_001420423.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z2G6.
SMR	A7Z2G6. Positions 1-251.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z2G6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000007683; EBBACP00000007561; EBBACG00000007675.
GeneID	5462832.
GenomeReviews	Gene locus RBAM_008080 in contig CP000560_GR.
KEGG	bay:RBAM_008080.
PATRIC	18746573. VBIBacAmy31356_0785.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0710.
GeneTree	EBGT00050000001972.
HOGENOM	HBG732926.
OMA	IAKIAVM.
ProtClustDB	PRK02412.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_008080-MONOMER.
Family and domain databases
HAMAP	MF_00214. AroD. [Tree]
InterPro	IPR018508. 3-dehydroquinate_DH_AS. IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K03785.
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
TIGRFAMs	TIGR01093. AroD. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_BACA2

Accession

Primary (citable) accession number: A7Z2G6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program