A7Z251 (PUR5_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	RBAM_006920

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_B
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00741_B.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_B		PRO_1000046420

Sequences

	Sequence		Length	Mass (Da)
	A7Z251 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: B06A010EF79C3951	FASTA	346	36,933
	10 20 30 40 50 60 MSDAYKNAGV DIEAGYEAVK RMKKHVERTQ RLGVMGSLGG FGGMFDLSEL PYQKPVLISG 70 80 90 100 110 120 TDGVGTKLKL AFSMDKHDTI GVDAVAMCVN DVLAQGAEPL FFLDYLAVGK ADPVKIEQIV 130 140 150 160 170 180 QGVADGCEQS GSALIGGETA EMPGLYTADE YDIAGFSVGV AEKDEIVTGE HIEEGHLLIG 190 200 210 220 230 240 LTSSGLHSNG FSLVRKVLLD DGGLDLDTVY EPFARPLGEE LLEPTRIYVK PVLKAVKSGK 250 260 270 280 290 300 VDGMAHVTGG GFIENIPRML PDGLSAEIDH GSWPIPPIFP FLQEHGKLKE EEMFNVFNMG 310 320 330 340 IGFVLAVKEE DLTGVIDTLE AQGEKAYLIG RVKRGEGISF GGAALS

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS73077.1.
RefSeq	YP_001420308.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z251.
SMR	A7Z251. Positions 13-340.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z251.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000005398; EBBACP00000005276; EBBACG00000005390.
GeneID	5460995.
GenomeReviews	Gene locus RBAM_006920 in contig CP000560_GR.
KEGG	bay:RBAM_006920.
PATRIC	18746318. VBIBacAmy31356_0658.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0150.
GeneTree	EBGT00050000002660.
HOGENOM	HBG531222.
OMA	GIDMIAM.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_006920-MONOMER.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
KO	K01933.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. PurM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_BACA2

Accession

Primary (citable) accession number: A7Z251

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program