Reviewed, UniProtKB/Swiss-Prot A7Z249 (PURL_BACA2)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase 2
    EC=6.3.5.3
Alternative name(s):
    Phosphoribosylformylglycinamidine synthase II
      Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	RBAM_006900

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	742 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, purQ and purL. HAMAP MF_00420
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 742	742	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000050296
Regions
	Nucleotide binding	111 – 122	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A7Z249-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 548F52808E025161	FASTA	742	80,111
	10 20 30 40 50 60 MSLLLEPSKE QIKEEKLYQQ MGVSDDEFAL IESIIGRLPN YTEIGIFSVM WSEHCSYKNS 70 80 90 100 110 120 KPVLRKFPTS GERVLQGPGE GAGIVDIGDN QAVVFKIESH NHPSAIEPYQ GAATGVGGII 130 140 150 160 170 180 RDVFSMGARP IAVLNSLRFG ELTSPRVKYL FEEVVAGIAG YGNCIGIPTV GGEVQFDASY 190 200 210 220 230 240 EGNPLVNAMC VGLIDHKDIK KGQAKGVGNT VMYVGAKTGR DGIHGATFAS EEMSDSSEEK 250 260 270 280 290 300 RSAVQVGDPF MEKLLLEACL EVIQCDALVG IQDMGAAGLT SSSAEMASKA GSGIEMNLDL 310 320 330 340 350 360 IPQRETGMTA YEMMLSESQE RMLLVIERGR EQEIVDIFDK YDLEAVSVGH VTDDKMLRLR 370 380 390 400 410 420 HNGEVVCELP VDALAEEAPV YHKPSAEPAY YREFQETEAP APEVKDATET LFALLQQPTI 430 440 450 460 470 480 ASKEWVYDQY DYMVRTNTVV APGSDAGVLR IRGTKKALAM TTDCNARYLY LDPEEGGKIA 490 500 510 520 530 540 VAEAARNIVC SGAEPLAVTD NLNFGNPEKP EIFWQIEKAA DGISEACNVL STPVIGGNVS 550 560 570 580 590 600 LYNESNGTAI YPTPVIGMVG LIEDTAHITT QHFKQAGDLV YVIGETKPEF AGSELQKMTE 610 620 630 640 650 660 GRIYGKAPQI DLDIELSRQK ALLDAIKKGF VQSAHDVSEG GLGVAIAESV MTTENLGANV 670 680 690 700 710 720 TVEGEAALLF SESQSRFVVS VKKEHQAAFE AAVADAVHIG EVTADGLLAI QNQDGQQLVH 730 740 AQTKELERAW KGAIPCLLKS KA

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000560 Genomic DNA. Translation: ABS73075.1.
RefSeq	YP_001420306.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z249.
Genome annotation databases
GeneID	5460993.
GenomeReviews	Gene locus RBAM_006900 in contig CP000560_GR.
KEGG	bay:RBAM_006900.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GNCVGVP.
Family and domain databases
HAMAP	MF_00420. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_BACA2

Accession

Primary (citable) accession number: A7Z249

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)