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A7Z1L3 (DDL_BACA2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:RBAM_004900
OrganismBacillus amyloliquefaciens subsp. plantarum (strain DSM 23117 / BGSC 10A6 / FZB42) [Complete proteome] [HAMAP]
Taxonomic identifier326423 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 354354D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341058

Regions

Domain133 – 338206ATP-grasp
Nucleotide binding166 – 22156ATP By similarity

Sites

Metal binding2921Magnesium or manganese 1 By similarity
Metal binding3051Magnesium or manganese 1 By similarity
Metal binding3051Magnesium or manganese 2 By similarity
Metal binding3071Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A7Z1L3 [UniParc].

Last modified October 23, 2007. Version 1.
Checksum: E55EAFAD7042E84C

FASTA35439,216
        10         20         30         40         50         60 
MYGGKSAEHN VSLQTALAVT KALNTEKFDI HPIYITEKGE WQRGPLLTEP VSNVKMLQLE 

        70         80         90        100        110        120 
QNGESFALSA LNREMFPEAS PDEKAPIDVV FPLLHGPNGE DGTIQGLLEL LNVPYVGNGV 

       130        140        150        160        170        180 
LASSAGMDKV IMKHLFAQAG LPQAKYAAFL KKDWKQTPDS CLQQVEKELG YPCFVKPANL 

       190        200        210        220        230        240 
GSSVGISKCR NREELEKAFE LAFEYDRKIV VEEGIAGREI EIGVLGNDEP KCSAVGEIAP 

       250        260        270        280        290        300 
KTDFYDYKAK YEDGDTDLMI PAQVTDEQYA EISEMAIKAF KAIDGSGLVR ADFFLTNDGQ 

       310        320        330        340        350 
VLINEVNTMP GFTPFSMFPL LWKEAGVDYS DLIEQLVELA KERHAEKQLI KHTF 

« Hide

References

[1]"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42."
Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R.
Nat. Biotechnol. 25:1007-1014(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 23117 / BGSC 10A6 / FZB42.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000560 Genomic DNA. Translation: ABS72889.1.
RefSeqYP_001420120.1. NC_009725.1.

3D structure databases

ProteinModelPortalA7Z1L3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING326423.RBAM_004900.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS72889; ABS72889; RBAM_004900.
GeneID5460378.
KEGGbay:RBAM_004900.
PATRIC18745902. VBIBacAmy31356_0463.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycBAMY326423:GCM4-488-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_BACA2
AccessionPrimary (citable) accession number: A7Z1L3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: October 23, 2007
Last modified: April 16, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways