A7Z0L6 (SYC_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	RBAM_001190

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Cysteine--tRNA ligase HAMAP MF_00041		PRO_0000332787
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	266 – 270	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	209	1	Zinc By similarity
	Metal binding	234	1	Zinc By similarity
	Metal binding	238	1	Zinc By similarity
	Binding site	269	1	ATP By similarity
Amino acid modifications
	Modified residue	270	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z0L6 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: 1C8DF21A1F96F036	FASTA	466	53,774
	10 20 30 40 50 60 MTITLYNTLT RKKETFVPLE EGKVKMYVCG PTVYNYIHIG NARPAIVYDT VRNYLEYKGY 70 80 90 100 110 120 DVQYVSNFTD VDDKLIKAAN ELGEDVPTVS ERFIKAYFED VGALGCRKAD LHPRVMENMD 130 140 150 160 170 180 AIIEFVSELI KKGYAYESEG DVYFKTRAFE GYGKLSQQSI DELRSGARIR VGEKKEDALD 190 200 210 220 230 240 FALWKAAKDG EISWDSPWGK GRPGWHIECS AMVKKYLGDE IDIHAGGQDL TFPHHENEIA 250 260 270 280 290 300 QSEALTGKTF AKYWLHNGYI NIDNEKMSKS LGNFVLVHDI IKQYDPQLLR FFMLSVHYRH 310 320 330 340 350 360 PINYSEELLE KTKNAFNRLK TAYSNLNHRL ISSTNLTDND EEWLAKIEEH RTAFEEAMDD 370 380 390 400 410 420 DFNTANAISV WFDLAKVANY YMQEDNTADH VIKAFISMFD RIGSVLGLTL GEEELVDEEI 430 440 450 460 EELIEKRNEA RRNRDFALSD QIRDQLKSMN IILEDTAQGT RWKRGE

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS72542.1.
RefSeq	YP_001419773.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z0L6.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z0L6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000006386; EBBACP00000006264; EBBACG00000006378.
GeneID	5460827.
GenomeReviews	Gene locus RBAM_001190 in contig CP000560_GR.
KEGG	bay:RBAM_001190.
PATRIC	18745135. VBIBacAmy31356_0101.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
GeneTree	EBGT00050000000801.
HOGENOM	HBG327651.
OMA	DFDALNM.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_001190-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_BACA2

Accession

Primary (citable) accession number: A7Z0L6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program