A7Z0L4 (SYE_BACA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	RBAM_001170

Organism

Bacillus amyloliquefaciens (strain FZB42) [Complete proteome] [HAMAP]

Taxonomic identifier

326423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 483	483	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_1000001871
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_00022_B
	Motif	252 – 256	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	255	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7Z0L4 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: F8CE741A129B45BC	FASTA	483	55,731
	10 20 30 40 50 60 MGNEVRVRYA PSPTGHLHIG NARTALFNYL FARSQGGKFI IRIEDTDKKR NIEGGEQSQL 70 80 90 100 110 120 NYLKWLGMDW DESVDIGGEY GPYRQSERND IYKKYYEELL EKGLAYKCFC TEEELEKERE 130 140 150 160 170 180 EQIARGEMPR YSGKHRNLTQ EEQERFLAEG RQPSIRFRVP EGKIIAFNDI VKGEISFESD 190 200 210 220 230 240 GIGDFVIVKK DGTPTYNFAV AIDDYLMKMT HVLRGEDHIS NTPKQIMIFQ AFGWDVPVFG 250 260 270 280 290 300 HMTLIVNESR KKLSKRDESI IQFIEQYDEL GYLPEALFNF IGLLGWSPVG EEELFTREQF 310 320 330 340 350 360 IEIFDVNRLS KSPALFDMHK LKWVNNQYVK ALDLDQVVEL TLPHLQKAGK VGTELSAEEQ 370 380 390 400 410 420 EWVRKLISLY HEQLSYGAEI VELTDLFFKD EIEYNQEAKA VLEEEQVPEV LSVFAAKLEE 430 440 450 460 470 480 LEEFTPELIK ASIKAVQKET GHKGKKLFMP IRVAVTGQTH GPELPQAIEL IGRETAVRRL KSI

References

[1]

"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42." Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. Nat. Biotechnol. 25:1007-1014(2007) [PubMed: 17704766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FZB42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000560 Genomic DNA. Translation: ABS72540.1.
RefSeq	YP_001419771.1. NC_009725.1.
3D structure databases
ProteinModelPortal	A7Z0L4.
ModBase	Search...
Protein-protein interaction databases
STRING	A7Z0L4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000007707; EBBACP00000007585; EBBACG00000007699.
GeneID	5460825.
GenomeReviews	Gene locus RBAM_001170 in contig CP000560_GR.
KEGG	bay:RBAM_001170.
PATRIC	18745129. VBIBacAmy31356_0098.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
GeneTree	EBGT00050000002239.
HOGENOM	HBG628189.
OMA	DDFDMEI.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	BAMY326423:RBAM_001170-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K09698.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_BACA2

Accession

Primary (citable) accession number: A7Z0L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program