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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Bacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) (Bacillus amyloliquefaciens subsp. plantarum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei255ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAMY326423:G1G84-119-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:RBAM_001170
OrganismiBacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) (Bacillus amyloliquefaciens subsp. plantarum)
Taxonomic identifieri326423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus amyloliquefaciens group
Proteomesi
  • UP000001120 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000018711 – 483Glutamate--tRNA ligaseAdd BLAST483

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA7Z0L4
SMRiA7Z0L4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi11 – 21"HIGH" regionUniRule annotationAdd BLAST11
Motifi252 – 256"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK09698
OMAiAYYAFDT

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A7Z0L4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNEVRVRYA PSPTGHLHIG NARTALFNYL FARSQGGKFI IRIEDTDKKR
60 70 80 90 100
NIEGGEQSQL NYLKWLGMDW DESVDIGGEY GPYRQSERND IYKKYYEELL
110 120 130 140 150
EKGLAYKCFC TEEELEKERE EQIARGEMPR YSGKHRNLTQ EEQERFLAEG
160 170 180 190 200
RQPSIRFRVP EGKIIAFNDI VKGEISFESD GIGDFVIVKK DGTPTYNFAV
210 220 230 240 250
AIDDYLMKMT HVLRGEDHIS NTPKQIMIFQ AFGWDVPVFG HMTLIVNESR
260 270 280 290 300
KKLSKRDESI IQFIEQYDEL GYLPEALFNF IGLLGWSPVG EEELFTREQF
310 320 330 340 350
IEIFDVNRLS KSPALFDMHK LKWVNNQYVK ALDLDQVVEL TLPHLQKAGK
360 370 380 390 400
VGTELSAEEQ EWVRKLISLY HEQLSYGAEI VELTDLFFKD EIEYNQEAKA
410 420 430 440 450
VLEEEQVPEV LSVFAAKLEE LEEFTPELIK ASIKAVQKET GHKGKKLFMP
460 470 480
IRVAVTGQTH GPELPQAIEL IGRETAVRRL KSI
Length:483
Mass (Da):55,731
Last modified:October 23, 2007 - v1
Checksum:iF8CE741A129B45BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000560 Genomic DNA Translation: ABS72540.1
RefSeqiWP_011996189.1, NC_009725.1

Genome annotation databases

EnsemblBacteriaiABS72540; ABS72540; RBAM_001170
KEGGibay:RBAM_001170

Similar proteinsi

Entry informationi

Entry nameiSYE_BACVZ
AccessioniPrimary (citable) accession number: A7Z0L4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 23, 2007
Last modified: May 23, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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