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Protein

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Gene

ispD

Organism
Bacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) (Bacillus amyloliquefaciens subsp. plantarum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).UniRule annotation

Catalytic activityi

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 2 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei15Transition state stabilizerUniRule annotation1
Sitei22Transition state stabilizerUniRule annotation1
Sitei152Positions MEP for the nucleophilic attackUniRule annotation1
Sitei209Positions MEP for the nucleophilic attackUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processIsoprene biosynthesis

Enzyme and pathway databases

BioCyciBAMY326423:G1G84-117-MONOMER
UniPathwayiUPA00056; UER00093

Names & Taxonomyi

Protein namesi
Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferaseUniRule annotation (EC:2.7.7.60UniRule annotation)
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthaseUniRule annotation
MEP cytidylyltransferaseUniRule annotation
Short name:
MCTUniRule annotation
Gene namesi
Name:ispDUniRule annotation
Ordered Locus Names:RBAM_001150
OrganismiBacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) (Bacillus amyloliquefaciens subsp. plantarum)
Taxonomic identifieri326423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus amyloliquefaciens group
Proteomesi
  • UP000001120 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000228971 – 2322-C-methyl-D-erythritol 4-phosphate cytidylyltransferaseAdd BLAST232

Structurei

3D structure databases

ProteinModelPortaliA7Z0L2
SMRiA7Z0L2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108QHG Bacteria
COG1211 LUCA
HOGENOMiHOG000218563
KOiK00991
OMAiERQHSVY

Family and domain databases

CDDicd02516 CDP-ME_synthetase, 1 hit
Gene3Di3.90.550.10, 1 hit
HAMAPiMF_00108 IspD, 1 hit
InterProiView protein in InterPro
IPR001228 IspD
IPR034683 IspD/TarI
IPR018294 ISPD_synthase_CS
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF01128 IspD, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR00453 ispD, 1 hit
PROSITEiView protein in PROSITE
PS01295 ISPD, 1 hit

Sequencei

Sequence statusi: Complete.

A7Z0L2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYVVVIPAA GQGKRMKAGK NKLFISLKGA PVIIHTLRVF ESHAPCQKII
60 70 80 90 100
LVINESERED FRQLLAQFPI QTEIELVTGG AERQHSVYEG LKMIDKESVV
110 120 130 140 150
LVHDGARPFV THQHIDKLVE TAEEAGAAVL AVPVKDTIKR AEGSRVAETF
160 170 180 190 200
DRSSLWAVQT PQAFRLSLLK TAHMEAENKG FLGTDDASLV ERLDGYQVSI
210 220 230
VEGSYTNIKL TTPDDLIFAE AIMKAESGNH NV
Length:232
Mass (Da):25,590
Last modified:October 23, 2007 - v1
Checksum:i2B2EDB7DCB15692B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000560 Genomic DNA Translation: ABS72538.1
RefSeqiWP_011996188.1, NC_009725.1

Genome annotation databases

EnsemblBacteriaiABS72538; ABS72538; RBAM_001150
KEGGibay:RBAM_001150

Similar proteinsi

Entry informationi

Entry nameiISPD_BACVZ
AccessioniPrimary (citable) accession number: A7Z0L2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 23, 2007
Last modified: April 25, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health