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Protein

WASH complex subunit 1

Gene

WASHC1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Its assembly in the WASH core complex seems to inhibit its NPF activity and via WASHC2 is required for its membrane targeting. Involved in endocytic trafficking of EGF. Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration. In T-cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T-cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation. Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
WASH complex subunit 1By similarity
Alternative name(s):
WAS protein family homolog 1
Gene namesi
Name:WASHC1By similarity
Synonyms:WASH1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 5

Subcellular locationi

  • Early endosome membrane By similarity
  • Recycling endosome membrane By similarity
  • Late endosome By similarity
  • Cytoplasmic vesicleautophagosome By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity

  • Note: Localization to the endosome membrane is mediated via its interaction with WASHC2.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003909611 – 471WASH complex subunit 1Add BLAST471

Proteomic databases

PaxDbiA7Z063.
PeptideAtlasiA7Z063.

Expressioni

Gene expression databases

BgeeiENSBTAG00000011902.

Interactioni

Subunit structurei

Component of the WASH core complex also described as WASH regulatory complex SHRC composed of WASHC1, WASHC2, WASHC3, WASHC4 and WASHC5. The WASH core complex associates with the F-actin-capping protein dimer (formed by CAPZA1, CAPZA2 or CAPZA3 and CAPZB); the assembly has been initially described as WASH complex (PubMed:20498093). Interacts (via WHD1 region) with WASHC2; the interaction is direct. Interacts with alpha-tubulin. Interacts with BECN1; WASHC1 and AMBRA1 can competetively interact with BECN1. Interacts with BLOC1S2; may associate with the BLOC-1 complex. Interacts with tubulin gamma chain (TUBG1 or TUBG2) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000015790.

Structurei

3D structure databases

ProteinModelPortaliA7Z063.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini365 – 387WH2PROSITE-ProRule annotationAdd BLAST23

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 167WHD1Add BLAST167
Regioni1 – 54Required for WASH complex assemblyBy similarityAdd BLAST54
Regioni353 – 471VCABy similarityAdd BLAST119

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi268 – 337Pro-richAdd BLAST70

Domaini

The VCA (verprolin, cofilin, acidic) domain promotes actin polymerization by the Arp2/3 complex in vitro.By similarity

Sequence similaritiesi

Belongs to the WASH1 family.Curated

Phylogenomic databases

eggNOGiENOG410IFZ4. Eukaryota.
ENOG410YN2V. LUCA.
GeneTreeiENSGT00390000016717.
HOGENOMiHOG000007381.
HOVERGENiHBG066686.
InParanoidiA7Z063.
KOiK18461.
OMAiTHTTLET.
OrthoDBiEOG091G0GKX.
TreeFamiTF318222.

Family and domain databases

InterProiView protein in InterPro
IPR028290. WASH1.
IPR021854. WASH1_WAHD.
IPR003124. WH2_dom.
PANTHERiPTHR23331. PTHR23331. 1 hit.
PfamiView protein in Pfam
PF11945. WASH_WAHD. 1 hit.
PROSITEiView protein in PROSITE
PS51082. WH2. 1 hit.

Sequencei

Sequence statusi: Complete.

A7Z063-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPTGTQHSL AGQTYAVPLI QPDLRREEAI QQVADALQYL QKVSGDIFSR
60 70 80 90 100
ISQRVELSRS QLQAIGERVS LAQAKIEKIK GSKKAIKVFS SAKYPAPERL
110 120 130 140 150
QEYGSIFMGA QDPGLQRRPR HRIQSKHRPL DERALQEKLK FFPVCVNTKP
160 170 180 190 200
EPEDEAEEGL GGLPSNISSV SSLLLFNTTE NLYKKYVFLD PLAGAVTKTH
210 220 230 240 250
VMLGAETEEK LFDAPLSISR REQLERQVPE NYFYVPDLGQ VPDIDVPSYL
260 270 280 290 300
PDLPGVADDL MYSADLGPGI APSAPGAIPE LPTFHTEVAQ PFKPDLEDGV
310 320 330 340 350
LTARPPPPPP PPPPPAPAVL MSVPPPPPPP QAPPGQPAKG DDSGGASPSA
360 370 380 390 400
PVQGAPKEVV DPSSGRATLL ESIRQAGGIG KAKLRSVKER KLEKKKQKEQ
410 420 430 440 450
EQVRATSQGG DLMSDLFNKL AMRRKGISGK GPGSGASEGP GGAFARMSDS
460 470
IPPLPPPQQP PGEEDEDDWE S
Length:471
Mass (Da):50,773
Last modified:October 23, 2007 - v1
Checksum:i15F8DDCF10B5816A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC153261 mRNA. Translation: AAI53262.1.
RefSeqiNP_001098876.1. NM_001105406.1.
UniGeneiBt.35980.

Genome annotation databases

EnsembliENSBTAT00000015790; ENSBTAP00000015790; ENSBTAG00000011902.
GeneIDi533602.
KEGGibta:533602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC153261 mRNA. Translation: AAI53262.1.
RefSeqiNP_001098876.1. NM_001105406.1.
UniGeneiBt.35980.

3D structure databases

ProteinModelPortaliA7Z063.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000015790.

Proteomic databases

PaxDbiA7Z063.
PeptideAtlasiA7Z063.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000015790; ENSBTAP00000015790; ENSBTAG00000011902.
GeneIDi533602.
KEGGibta:533602.

Organism-specific databases

CTDi555174.

Phylogenomic databases

eggNOGiENOG410IFZ4. Eukaryota.
ENOG410YN2V. LUCA.
GeneTreeiENSGT00390000016717.
HOGENOMiHOG000007381.
HOVERGENiHBG066686.
InParanoidiA7Z063.
KOiK18461.
OMAiTHTTLET.
OrthoDBiEOG091G0GKX.
TreeFamiTF318222.

Gene expression databases

BgeeiENSBTAG00000011902.

Family and domain databases

InterProiView protein in InterPro
IPR028290. WASH1.
IPR021854. WASH1_WAHD.
IPR003124. WH2_dom.
PANTHERiPTHR23331. PTHR23331. 1 hit.
PfamiView protein in Pfam
PF11945. WASH_WAHD. 1 hit.
PROSITEiView protein in PROSITE
PS51082. WH2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiWASH1_BOVIN
AccessioniPrimary (citable) accession number: A7Z063
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: October 23, 2007
Last modified: March 15, 2017
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.