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Protein

WAS protein family homolog 1

Gene

WASH1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Its assembly in the WASH core complex seems to inhibit its NPF activity and via FAM21 is required for its membrane targeting. Involved in endocytic trafficking of EGF. Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration. In T-cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T-cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation. Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
WAS protein family homolog 1
Gene namesi
Name:WASH1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 5

Subcellular locationi

  • Early endosome membrane By similarity
  • Recycling endosome membrane By similarity
  • Late endosome By similarity
  • Cytoplasmic vesicleautophagosome By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity

  • Note: Localization to the endosome membrane is mediated via its interaction with FAM21.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 471471WAS protein family homolog 1PRO_0000390961Add
BLAST

Proteomic databases

PaxDbiA7Z063.
PRIDEiA7Z063.

Interactioni

Subunit structurei

Component of the WASH core complex also known as WASH regulatory complex SHRC composed of WASH1, FAM21, KIAA1033/SWIP, KIAA0196/Strumpellin and CCDC53. The WASH core complex associates with the F-actin-capping protein dimer (formed by CAPZA1, CAPZA2 or CAPZA3 and CAPZB); the assembly has been initially described as WASH complex (PubMed:20498093). Interacts (via WHD1 region) with FAM21; the interaction is direct. Interacts with alpha-tubulin. Interacts with BECN1; WASH1 and AMBRA1 can competetively interact with BECN1. Interacts with BLOC1S2; may associate with the BLOC-1 complex. Interacts with tubulin gamma chain (TUBG1 or TUBG2) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000015790.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini365 – 38723WH2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 167167WHD1Add
BLAST
Regioni1 – 5454Required for WASH complex assemblyBy similarityAdd
BLAST
Regioni353 – 471119VCABy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi268 – 33770Pro-richAdd
BLAST

Domaini

The VCA (verprolin, cofilin, acidic) domain promotes actin polymerization by the Arp2/3 complex in vitro.By similarity

Sequence similaritiesi

Belongs to the WASH1 family.Curated
Contains 1 WH2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IFZ4. Eukaryota.
ENOG410YN2V. LUCA.
GeneTreeiENSGT00390000016717.
HOGENOMiHOG000007381.
HOVERGENiHBG066686.
InParanoidiA7Z063.
KOiK18461.
OMAiTHTTLET.
OrthoDBiEOG79PJPR.
TreeFamiTF318222.

Family and domain databases

InterProiIPR028290. WASH1.
IPR021854. WASH1_WAHD.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23331. PTHR23331. 1 hit.
PfamiPF11945. WASH_WAHD. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7Z063-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPTGTQHSL AGQTYAVPLI QPDLRREEAI QQVADALQYL QKVSGDIFSR
60 70 80 90 100
ISQRVELSRS QLQAIGERVS LAQAKIEKIK GSKKAIKVFS SAKYPAPERL
110 120 130 140 150
QEYGSIFMGA QDPGLQRRPR HRIQSKHRPL DERALQEKLK FFPVCVNTKP
160 170 180 190 200
EPEDEAEEGL GGLPSNISSV SSLLLFNTTE NLYKKYVFLD PLAGAVTKTH
210 220 230 240 250
VMLGAETEEK LFDAPLSISR REQLERQVPE NYFYVPDLGQ VPDIDVPSYL
260 270 280 290 300
PDLPGVADDL MYSADLGPGI APSAPGAIPE LPTFHTEVAQ PFKPDLEDGV
310 320 330 340 350
LTARPPPPPP PPPPPAPAVL MSVPPPPPPP QAPPGQPAKG DDSGGASPSA
360 370 380 390 400
PVQGAPKEVV DPSSGRATLL ESIRQAGGIG KAKLRSVKER KLEKKKQKEQ
410 420 430 440 450
EQVRATSQGG DLMSDLFNKL AMRRKGISGK GPGSGASEGP GGAFARMSDS
460 470
IPPLPPPQQP PGEEDEDDWE S
Length:471
Mass (Da):50,773
Last modified:October 23, 2007 - v1
Checksum:i15F8DDCF10B5816A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC153261 mRNA. Translation: AAI53262.1.
RefSeqiNP_001098876.1. NM_001105406.1.
UniGeneiBt.35980.

Genome annotation databases

EnsembliENSBTAT00000015790; ENSBTAP00000015790; ENSBTAG00000011902.
GeneIDi533602.
KEGGibta:533602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC153261 mRNA. Translation: AAI53262.1.
RefSeqiNP_001098876.1. NM_001105406.1.
UniGeneiBt.35980.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000015790.

Proteomic databases

PaxDbiA7Z063.
PRIDEiA7Z063.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000015790; ENSBTAP00000015790; ENSBTAG00000011902.
GeneIDi533602.
KEGGibta:533602.

Organism-specific databases

CTDi100287171.

Phylogenomic databases

eggNOGiENOG410IFZ4. Eukaryota.
ENOG410YN2V. LUCA.
GeneTreeiENSGT00390000016717.
HOGENOMiHOG000007381.
HOVERGENiHBG066686.
InParanoidiA7Z063.
KOiK18461.
OMAiTHTTLET.
OrthoDBiEOG79PJPR.
TreeFamiTF318222.

Miscellaneous databases

NextBioi20876085.

Family and domain databases

InterProiIPR028290. WASH1.
IPR021854. WASH1_WAHD.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23331. PTHR23331. 1 hit.
PfamiPF11945. WASH_WAHD. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Ascending colon.
  2. "WASH and WAVE actin regulators of the Wiskott-Aldrich syndrome protein (WASP) family are controlled by analogous structurally related complexes."
    Jia D., Gomez T.S., Metlagel Z., Umetani J., Otwinowski Z., Rosen M.K., Billadeau D.D.
    Proc. Natl. Acad. Sci. U.S.A. 107:10442-10447(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE WASH COMPLEX.

Entry informationi

Entry nameiWASH1_BOVIN
AccessioniPrimary (citable) accession number: A7Z063
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: October 23, 2007
Last modified: May 11, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.