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Protein

Ubiquitin carboxyl-terminal hydrolase 20

Gene

USP20

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei154 – 1541NucleophilePROSITE-ProRule annotation
Active sitei641 – 6411Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Endocytosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 20 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 20
Ubiquitin thioesterase 20
Ubiquitin-specific-processing protease 20
Gene namesi
Name:USP20
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 11

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 912912Ubiquitin carboxyl-terminal hydrolase 20PRO_0000390417Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei132 – 1321PhosphoserineBy similarity
Modified residuei134 – 1341PhosphoserineBy similarity
Modified residuei259 – 2591PhosphothreonineBy similarity
Modified residuei369 – 3691PhosphoserineBy similarity
Modified residuei378 – 3781PhosphothreonineBy similarity
Modified residuei413 – 4131PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiA7Z056.

Interactioni

Subunit structurei

Interacts with VHL, leading to its ubiquitination and subsequent degradation. Interacts with CCP110, DIO2 and HIF1A (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027070.

Structurei

3D structure databases

ProteinModelPortaliA7Z056.
SMRiA7Z056. Positions 5-99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini145 – 683539USPAdd
BLAST
Domaini685 – 77894DUSP 1PROSITE-ProRule annotationAdd
BLAST
Domaini787 – 890104DUSP 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue (By similarity).By similarity

Sequence similaritiesi

Contains 2 DUSP domains.PROSITE-ProRule annotation
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5560.
GeneTreeiENSGT00790000122967.
HOGENOMiHOG000286031.
HOVERGENiHBG054196.
InParanoidiA7Z056.
KOiK11848.
OMAiWGQQRTN.
OrthoDBiEOG7CRTP2.
TreeFamiTF352179.

Family and domain databases

Gene3Di3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF06337. DUSP. 1 hit.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 2 hits.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 2 hits.
PROSITEiPS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7Z056-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDSRDLCPH LDSIGEVTKE DLLLKSKSTC QSCGVSGPNL WACLQVSCSY
60 70 80 90 100
VGCGESFADH STLHAQAKKH NLTVNLTTFR VWCYACEKEV FLEPRLAAHP
110 120 130 140 150
PGPAPKFSEQ DSPPPSHPLK AVPIAVADEG ESESEDDDLK PRGLTGMKNL
160 170 180 190 200
GNSCYMNAAL QALSNCPPLT QFFLECGGLV RTDKKPALCK SYQKLVSEVW
210 220 230 240 250
HRKRPSYVVP TSLSHGIKLV NPMFRGYAQQ DTQEFLRCLM DQLHEELKEP
260 270 280 290 300
VVATAAALTE ARDSDSSDTD EKREGDRSPS EDEFLSCDSS SDRGEGDGQG
310 320 330 340 350
RSGGGSQAEA ELLMADEAGR AISEKERMKD RKFSWGQQRT NSEQVDEDAD
360 370 380 390 400
VDTAMAALEQ QPPETQPPSP RSTSPCRTPE PDNEAHMRSS RPCSPVHHHE
410 420 430 440 450
GHAKLASSPH RASPVRMGPA YVLKKAQVPG SRRRKEQSYR SVISDIFDGS
460 470 480 490 500
VLSLVQCLTC DRVSTTVETF QDLSLPIPGK EDLAKLHSAI YQNVPAKPGA
510 520 530 540 550
CGDSYVAQGW LAFIVEYIRR FVVSCTPSWF WGPVVTLEDC LAAFFAADEL
560 570 580 590 600
KGDNMYSCER CKKLRNGVKY CKVLRLPEIL CIHLKRFRHE VMYSFKISSH
610 620 630 640 650
VSFPLEGLDL RPFLAKECTS QITTYDLLSV ICHHGTAGSG HYIAYCQNVI
660 670 680 690 700
NGQWYEFDDQ YVTEVHETVV QNAEAYVLFY RKSSEEAVRE RQQVVSLAAM
710 720 730 740 750
REPSLLRFYV SREWLNKFNT FAEPGPITNH TFLCSHGGIP PNKYHYIDDL
760 770 780 790 800
VVILPQNVWE HLYSRFGGGP AVNHLYVCSI CQVEIEALAK RRRVEIDTFI
810 820 830 840 850
KLNKAFQAEE SPSVIYCISM QWFREWEAFV KGKDNEPPGP IDNSRIAQVK
860 870 880 890 900
GSGHIQLKPG ADYGQISEET WVYLNNLYGG GPEIAIRQSV AQLPDPESLH
910
GEQKIEAETR AL
Length:912
Mass (Da):101,815
Last modified:October 23, 2007 - v1
Checksum:iA2E4A254D3C95EDF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC153254 mRNA. Translation: AAI53255.1.
RefSeqiNP_001098803.1. NM_001105333.1.
UniGeneiBt.26746.

Genome annotation databases

EnsembliENSBTAT00000027070; ENSBTAP00000027070; ENSBTAG00000020311.
GeneIDi505839.
KEGGibta:505839.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC153254 mRNA. Translation: AAI53255.1.
RefSeqiNP_001098803.1. NM_001105333.1.
UniGeneiBt.26746.

3D structure databases

ProteinModelPortaliA7Z056.
SMRiA7Z056. Positions 5-99.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027070.

Proteomic databases

PRIDEiA7Z056.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000027070; ENSBTAP00000027070; ENSBTAG00000020311.
GeneIDi505839.
KEGGibta:505839.

Organism-specific databases

CTDi10868.

Phylogenomic databases

eggNOGiCOG5560.
GeneTreeiENSGT00790000122967.
HOGENOMiHOG000286031.
HOVERGENiHBG054196.
InParanoidiA7Z056.
KOiK11848.
OMAiWGQQRTN.
OrthoDBiEOG7CRTP2.
TreeFamiTF352179.

Miscellaneous databases

NextBioi20867329.

Family and domain databases

Gene3Di3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF06337. DUSP. 1 hit.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 2 hits.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 2 hits.
PROSITEiPS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Uterus.

Entry informationi

Entry nameiUBP20_BOVIN
AccessioniPrimary (citable) accession number: A7Z056
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: October 23, 2007
Last modified: July 22, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.