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A7Z056

- UBP20_BOVIN

UniProt

A7Z056 - UBP20_BOVIN

Protein

Ubiquitin carboxyl-terminal hydrolase 20

Gene

USP20

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (23 Oct 2007)
      Previous versions | rss
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    Functioni

    Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei154 – 1541NucleophilePROSITE-ProRule annotation
    Active sitei641 – 6411Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: UniProtKB
    2. ubiquitin-specific protease activity Source: InterPro
    3. ubiquitin thiolesterase activity Source: UniProtKB
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. endocytosis Source: UniProtKB-KW
    2. protein deubiquitination Source: UniProtKB
    3. protein K48-linked deubiquitination Source: UniProtKB
    4. protein K63-linked deubiquitination Source: UniProtKB
    5. regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
    6. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Endocytosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 20 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 20
    Ubiquitin thioesterase 20
    Ubiquitin-specific-processing protease 20
    Gene namesi
    Name:USP20
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 11

    Subcellular locationi

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 912912Ubiquitin carboxyl-terminal hydrolase 20PRO_0000390417Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei132 – 1321PhosphoserineBy similarity
    Modified residuei134 – 1341PhosphoserineBy similarity
    Modified residuei259 – 2591PhosphothreonineBy similarity
    Modified residuei369 – 3691PhosphoserineBy similarity
    Modified residuei378 – 3781PhosphothreonineBy similarity
    Modified residuei413 – 4131PhosphoserineBy similarity

    Post-translational modificationi

    Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiA7Z056.

    Interactioni

    Subunit structurei

    Interacts with VHL, leading to its ubiquitination and subsequent degradation. Interacts with CCP110, DIO2 and HIF1A By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000027070.

    Structurei

    3D structure databases

    ProteinModelPortaliA7Z056.
    SMRiA7Z056. Positions 5-99.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini145 – 683539USPAdd
    BLAST
    Domaini685 – 77894DUSP 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini787 – 890104DUSP 2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue By similarity.By similarity

    Sequence similaritiesi

    Contains 2 DUSP domains.PROSITE-ProRule annotation
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5560.
    GeneTreeiENSGT00750000117701.
    HOGENOMiHOG000286031.
    HOVERGENiHBG054196.
    InParanoidiA7Z056.
    KOiK11848.
    OMAiADYGQIS.
    OrthoDBiEOG7CRTP2.
    TreeFamiTF352179.

    Family and domain databases

    Gene3Di3.30.2230.10. 2 hits.
    3.30.40.10. 1 hit.
    InterProiIPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF06337. DUSP. 1 hit.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    SMARTiSM00695. DUSP. 2 hits.
    [Graphical view]
    SUPFAMiSSF143791. SSF143791. 2 hits.
    PROSITEiPS51283. DUSP. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7Z056-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGDSRDLCPH LDSIGEVTKE DLLLKSKSTC QSCGVSGPNL WACLQVSCSY    50
    VGCGESFADH STLHAQAKKH NLTVNLTTFR VWCYACEKEV FLEPRLAAHP 100
    PGPAPKFSEQ DSPPPSHPLK AVPIAVADEG ESESEDDDLK PRGLTGMKNL 150
    GNSCYMNAAL QALSNCPPLT QFFLECGGLV RTDKKPALCK SYQKLVSEVW 200
    HRKRPSYVVP TSLSHGIKLV NPMFRGYAQQ DTQEFLRCLM DQLHEELKEP 250
    VVATAAALTE ARDSDSSDTD EKREGDRSPS EDEFLSCDSS SDRGEGDGQG 300
    RSGGGSQAEA ELLMADEAGR AISEKERMKD RKFSWGQQRT NSEQVDEDAD 350
    VDTAMAALEQ QPPETQPPSP RSTSPCRTPE PDNEAHMRSS RPCSPVHHHE 400
    GHAKLASSPH RASPVRMGPA YVLKKAQVPG SRRRKEQSYR SVISDIFDGS 450
    VLSLVQCLTC DRVSTTVETF QDLSLPIPGK EDLAKLHSAI YQNVPAKPGA 500
    CGDSYVAQGW LAFIVEYIRR FVVSCTPSWF WGPVVTLEDC LAAFFAADEL 550
    KGDNMYSCER CKKLRNGVKY CKVLRLPEIL CIHLKRFRHE VMYSFKISSH 600
    VSFPLEGLDL RPFLAKECTS QITTYDLLSV ICHHGTAGSG HYIAYCQNVI 650
    NGQWYEFDDQ YVTEVHETVV QNAEAYVLFY RKSSEEAVRE RQQVVSLAAM 700
    REPSLLRFYV SREWLNKFNT FAEPGPITNH TFLCSHGGIP PNKYHYIDDL 750
    VVILPQNVWE HLYSRFGGGP AVNHLYVCSI CQVEIEALAK RRRVEIDTFI 800
    KLNKAFQAEE SPSVIYCISM QWFREWEAFV KGKDNEPPGP IDNSRIAQVK 850
    GSGHIQLKPG ADYGQISEET WVYLNNLYGG GPEIAIRQSV AQLPDPESLH 900
    GEQKIEAETR AL 912
    Length:912
    Mass (Da):101,815
    Last modified:October 23, 2007 - v1
    Checksum:iA2E4A254D3C95EDF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC153254 mRNA. Translation: AAI53255.1.
    RefSeqiNP_001098803.1. NM_001105333.1.
    XP_005213261.1. XM_005213204.1.
    XP_005213262.1. XM_005213205.1.
    UniGeneiBt.26746.

    Genome annotation databases

    EnsembliENSBTAT00000027070; ENSBTAP00000027070; ENSBTAG00000020311.
    GeneIDi505839.
    KEGGibta:505839.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC153254 mRNA. Translation: AAI53255.1 .
    RefSeqi NP_001098803.1. NM_001105333.1.
    XP_005213261.1. XM_005213204.1.
    XP_005213262.1. XM_005213205.1.
    UniGenei Bt.26746.

    3D structure databases

    ProteinModelPortali A7Z056.
    SMRi A7Z056. Positions 5-99.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000027070.

    Proteomic databases

    PRIDEi A7Z056.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000027070 ; ENSBTAP00000027070 ; ENSBTAG00000020311 .
    GeneIDi 505839.
    KEGGi bta:505839.

    Organism-specific databases

    CTDi 10868.

    Phylogenomic databases

    eggNOGi COG5560.
    GeneTreei ENSGT00750000117701.
    HOGENOMi HOG000286031.
    HOVERGENi HBG054196.
    InParanoidi A7Z056.
    KOi K11848.
    OMAi ADYGQIS.
    OrthoDBi EOG7CRTP2.
    TreeFami TF352179.

    Miscellaneous databases

    NextBioi 20867329.

    Family and domain databases

    Gene3Di 3.30.2230.10. 2 hits.
    3.30.40.10. 1 hit.
    InterProi IPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF06337. DUSP. 1 hit.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    SMARTi SM00695. DUSP. 2 hits.
    [Graphical view ]
    SUPFAMi SSF143791. SSF143791. 2 hits.
    PROSITEi PS51283. DUSP. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Uterus.

    Entry informationi

    Entry nameiUBP20_BOVIN
    AccessioniPrimary (citable) accession number: A7Z056
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: October 23, 2007
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3