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A7Z056

- UBP20_BOVIN

UniProt

A7Z056 - UBP20_BOVIN

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Protein

Ubiquitin carboxyl-terminal hydrolase 20

Gene
USP20
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei154 – 1541Nucleophile By similarity
Active sitei641 – 6411Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 9265UBP-typeAdd
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: UniProtKB
  2. ubiquitin-specific protease activity Source: InterPro
  3. ubiquitin thiolesterase activity Source: UniProtKB
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. endocytosis Source: UniProtKB-KW
  2. protein deubiquitination Source: UniProtKB
  3. protein K48-linked deubiquitination Source: UniProtKB
  4. protein K63-linked deubiquitination Source: UniProtKB
  5. regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
  6. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Endocytosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 20 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 20
Ubiquitin thioesterase 20
Ubiquitin-specific-processing protease 20
Gene namesi
Name:USP20
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 11

Subcellular locationi

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 912912Ubiquitin carboxyl-terminal hydrolase 20PRO_0000390417Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei132 – 1321Phosphoserine By similarity
Modified residuei134 – 1341Phosphoserine By similarity
Modified residuei259 – 2591Phosphothreonine By similarity
Modified residuei369 – 3691Phosphoserine By similarity
Modified residuei378 – 3781Phosphothreonine By similarity
Modified residuei413 – 4131Phosphoserine By similarity

Post-translational modificationi

Ubiquitinated via a VHL-dependent pathway for proteasomal degradation By similarity.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiA7Z056.

Interactioni

Subunit structurei

Interacts with VHL, leading to its ubiquitination and subsequent degradation. Interacts with CCP110, DIO2 and HIF1A By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027070.

Structurei

3D structure databases

ProteinModelPortaliA7Z056.
SMRiA7Z056. Positions 5-99.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini145 – 683539USPAdd
BLAST
Domaini685 – 77894DUSP 1Add
BLAST
Domaini787 – 890104DUSP 2Add
BLAST

Domaini

The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue By similarity.

Sequence similaritiesi

Contains 2 DUSP domains.
Contains 1 USP domain.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 9265UBP-typeAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5560.
GeneTreeiENSGT00750000117701.
HOGENOMiHOG000286031.
HOVERGENiHBG054196.
InParanoidiA7Z056.
KOiK11848.
OMAiADYGQIS.
OrthoDBiEOG7CRTP2.
TreeFamiTF352179.

Family and domain databases

Gene3Di3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF06337. DUSP. 1 hit.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 2 hits.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 2 hits.
PROSITEiPS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7Z056-1 [UniParc]FASTAAdd to Basket

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MGDSRDLCPH LDSIGEVTKE DLLLKSKSTC QSCGVSGPNL WACLQVSCSY    50
VGCGESFADH STLHAQAKKH NLTVNLTTFR VWCYACEKEV FLEPRLAAHP 100
PGPAPKFSEQ DSPPPSHPLK AVPIAVADEG ESESEDDDLK PRGLTGMKNL 150
GNSCYMNAAL QALSNCPPLT QFFLECGGLV RTDKKPALCK SYQKLVSEVW 200
HRKRPSYVVP TSLSHGIKLV NPMFRGYAQQ DTQEFLRCLM DQLHEELKEP 250
VVATAAALTE ARDSDSSDTD EKREGDRSPS EDEFLSCDSS SDRGEGDGQG 300
RSGGGSQAEA ELLMADEAGR AISEKERMKD RKFSWGQQRT NSEQVDEDAD 350
VDTAMAALEQ QPPETQPPSP RSTSPCRTPE PDNEAHMRSS RPCSPVHHHE 400
GHAKLASSPH RASPVRMGPA YVLKKAQVPG SRRRKEQSYR SVISDIFDGS 450
VLSLVQCLTC DRVSTTVETF QDLSLPIPGK EDLAKLHSAI YQNVPAKPGA 500
CGDSYVAQGW LAFIVEYIRR FVVSCTPSWF WGPVVTLEDC LAAFFAADEL 550
KGDNMYSCER CKKLRNGVKY CKVLRLPEIL CIHLKRFRHE VMYSFKISSH 600
VSFPLEGLDL RPFLAKECTS QITTYDLLSV ICHHGTAGSG HYIAYCQNVI 650
NGQWYEFDDQ YVTEVHETVV QNAEAYVLFY RKSSEEAVRE RQQVVSLAAM 700
REPSLLRFYV SREWLNKFNT FAEPGPITNH TFLCSHGGIP PNKYHYIDDL 750
VVILPQNVWE HLYSRFGGGP AVNHLYVCSI CQVEIEALAK RRRVEIDTFI 800
KLNKAFQAEE SPSVIYCISM QWFREWEAFV KGKDNEPPGP IDNSRIAQVK 850
GSGHIQLKPG ADYGQISEET WVYLNNLYGG GPEIAIRQSV AQLPDPESLH 900
GEQKIEAETR AL 912
Length:912
Mass (Da):101,815
Last modified:October 23, 2007 - v1
Checksum:iA2E4A254D3C95EDF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC153254 mRNA. Translation: AAI53255.1.
RefSeqiNP_001098803.1. NM_001105333.1.
XP_005213261.1. XM_005213204.1.
XP_005213262.1. XM_005213205.1.
UniGeneiBt.26746.

Genome annotation databases

EnsembliENSBTAT00000027070; ENSBTAP00000027070; ENSBTAG00000020311.
GeneIDi505839.
KEGGibta:505839.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC153254 mRNA. Translation: AAI53255.1 .
RefSeqi NP_001098803.1. NM_001105333.1.
XP_005213261.1. XM_005213204.1.
XP_005213262.1. XM_005213205.1.
UniGenei Bt.26746.

3D structure databases

ProteinModelPortali A7Z056.
SMRi A7Z056. Positions 5-99.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000027070.

Proteomic databases

PRIDEi A7Z056.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000027070 ; ENSBTAP00000027070 ; ENSBTAG00000020311 .
GeneIDi 505839.
KEGGi bta:505839.

Organism-specific databases

CTDi 10868.

Phylogenomic databases

eggNOGi COG5560.
GeneTreei ENSGT00750000117701.
HOGENOMi HOG000286031.
HOVERGENi HBG054196.
InParanoidi A7Z056.
KOi K11848.
OMAi ADYGQIS.
OrthoDBi EOG7CRTP2.
TreeFami TF352179.

Miscellaneous databases

NextBioi 20867329.

Family and domain databases

Gene3Di 3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProi IPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF06337. DUSP. 1 hit.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
SMARTi SM00695. DUSP. 2 hits.
[Graphical view ]
SUPFAMi SSF143791. SSF143791. 2 hits.
PROSITEi PS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Uterus.

Entry informationi

Entry nameiUBP20_BOVIN
AccessioniPrimary (citable) accession number: A7Z056
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: October 23, 2007
Last modified: April 16, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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