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Protein

Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1

Gene

PPIP5K1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Bifunctional inositol kinase that acts in concert with the IP6K kinases IP6K1, IP6K2 and IP6K3 to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, exocytosis, insulin signaling and neutrophil activation. Phosphorylates inositol hexakisphosphate (InsP6) at positions 1 or 3 to produce PP-InsP5 which is in turn phosphorylated by IP6Ks to produce (PP)2-InsP4. Alternatively, phosphorylates at position 1 or 3 PP-InsP5, produced by IP6Ks from InsP6, to produce (PP)2-InsP4. Activated when cells are exposed to hyperosmotic stress.By similarity

Catalytic activityi

ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate.
ATP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.
ATP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.
ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei145ATPBy similarity1
Binding sitei198ATPBy similarity1
Binding sitei205ATPBy similarity1
Binding sitei224ATPBy similarity1
Binding sitei259SubstrateBy similarity1
Binding sitei273SubstrateBy similarity1
Binding sitei275ATPBy similarity1
Binding sitei320ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi248 – 251ATPBy similarity4
Nucleotide bindingi257 – 259ATPBy similarity3
Nucleotide bindingi332 – 334ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-1855167. Synthesis of pyrophosphates in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1 (EC:2.7.4.21, EC:2.7.4.24)
Alternative name(s):
Diphosphoinositol pentakisphosphate kinase 1
Histidine acid phosphatase domain-containing protein 2A
InsP6 and PP-IP5 kinase 1
Gene namesi
Name:PPIP5K1
Synonyms:HISPPD2A, VIP1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 21

Subcellular locationi

  • Cytoplasmcytosol By similarity
  • Cell membrane By similarity

  • Note: Relocalizes to the plasma membrane upon activation of the PtdIns 3-kinase pathway.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003156871 – 1477Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1Add BLAST1477

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei940PhosphoserineBy similarity1
Modified residuei983PhosphoserineBy similarity1
Modified residuei1033PhosphoserineBy similarity1
Modified residuei1069PhosphoserineBy similarity1
Modified residuei1141PhosphoserineBy similarity1
Modified residuei1148PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiA7Z050.
PRIDEiA7Z050.

Expressioni

Gene expression databases

BgeeiENSBTAG00000014005.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000018635.

Structurei

3D structure databases

ProteinModelPortaliA7Z050.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni64 – 65Substrate bindingBy similarity2
Regioni224 – 225Substrate bindingBy similarity2
Regioni337 – 340Substrate bindingBy similarity4
Regioni382 – 453Polyphosphoinositide-binding domainBy similarityAdd BLAST72

Domaini

The C-terminal acid phosphatase-like domain binds PtdIns(3,4,5)P3 and InsP6. Despite its similarity with the phosphatase domain of histidine acid phosphatases, it has no phosphatase activity.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1057. Eukaryota.
ENOG410XNSN. LUCA.
GeneTreeiENSGT00390000009048.
HOGENOMiHOG000177917.
HOVERGENiHBG108657.
InParanoidiA7Z050.
KOiK13024.
OMAiMHSNQAS.
OrthoDBiEOG091G00ZU.
TreeFamiTF313594.

Family and domain databases

CDDicd07061. HP_HAP_like. 1 hit.
Gene3Di3.40.50.1240. 4 hits.
InterProiIPR033379. Acid_Pase_AS.
IPR000560. His_Pase_clade-2.
IPR029033. His_PPase_superfam.
IPR030138. PPIP5K1.
[Graphical view]
PANTHERiPTHR12750:SF11. PTHR12750:SF11. 1 hit.
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 3 hits.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7Z050-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWSLPASEGE SATAHFFLGA GDEGLGTRGL GMRPEESDSE LLEDEEDEVP
60 70 80 90 100
PEPQIIVGIC AMTKKSKSKP MTQILERLCR FDYLTVIILG EDVILNEPVE
110 120 130 140 150
NWPSCHCLIS FHSKGFPLDK AVAYSKLRNP FLINDLAMQY YIQDRREVYR
160 170 180 190 200
ILQEEGIDLP RYAVLNRDPA RPEECNLIEG EDQVEVNGAV FPKPFVEKPV
210 220 230 240 250
SAEDHNVYIY YPSSAGGGSQ RLFRKIGSRS SVYSPESSVR KTGSYIYEEF
260 270 280 290 300
MPTDGTDVKV YTVGPDYAHA EARKSPALDG KVERDSEGKE IRYPVMLTAM
310 320 330 340 350
EKLVARKVCV AFKQTVCGFD LLRANGHSFV CDVNGFSFVK NSMKYYDDCA
360 370 380 390 400
KILGNTIMRE LAPQFQIPWS IPMEAEDIPI VPTTSGTMME LRCVIAIIRH
410 420 430 440 450
GDRTPKQKMK MEVTHPRFFS LFEKHGGYKT GKLKLKRPEQ LQEVLDITRL
460 470 480 490 500
LLAELEKEPG GEIEEKTGKL EQLKSVLEMY GHFSGINRKV QLTYYPHGVK
510 520 530 540 550
ASNEGQDTQR EALAPSLLLV LKWGGELTPA GRVQAEELGR AFRCMYPGGQ
560 570 580 590 600
GDYAGFPGCG LLRLHSTFRH DLKIYASDEG RVQMTAAAFA KGLLALEGEL
610 620 630 640 650
TPILVQMVKS ANMNGLLDSD GDSLSSCQHR VKARLHHILQ QDAPFGPEDY
660 670 680 690 700
NQLAPTGSTS LLSSMAVIQN PVKVCDQVFD LIENLTHQIR ERMQDPKSVD
710 720 730 740 750
LQLYHSETLE LMLQRWSKLE RDFRQKSGRY DISKIPDIYD CVKYDVQHNG
760 770 780 790 800
SLGLQGTAEL LRLSKALADV VIPQEYGISR EEKLEIAVGF CLPLLRKILL
810 820 830 840 850
DLQRTHEDES VNKLHPLYSR GVLSPGRHVR TRLYFTSESH VHSLLSVFRY
860 870 880 890 900
GGLLDETKDT QWQRALAYLS AISELNYMTQ IVIMLYEDNT RDPLSEERFH
910 920 930 940 950
VELHFSPGVK GVEEEGSAPT GCGFRPASSE NEERKADQGS VEDLCPGKAS
960 970 980 990 1000
DEPDRALQTS PLPSEGPGLP KRSPLIRNRK AGSMEVLSET SSSRPGGHRL
1010 1020 1030 1040 1050
FSSSRPPTEM KQSGLGSQCT GLFSTTVLGG SSSAPNLQDY ARSQGKKLPP
1060 1070 1080 1090 1100
ASLKHRDELL FVPAVKRFSV SFAKHPTNGF EGCSMVPTIY PLETLHNALS
1110 1120 1130 1140 1150
LRQVSEFLSR VCQRHTEAQA QASAALFDSM HSNQASDSPF SPPRTLHSPT
1160 1170 1180 1190 1200
LQLQQRSEKP PWYSSGPSST VSSAGPSSPT AVDGNCPFGF SDQPSVSSHV
1210 1220 1230 1240 1250
TEEYQGLGLL QEAPGSGAQE PPLEGQQEPF EQNQSPQEPP VETKKPCQEV
1260 1270 1280 1290 1300
AEEVSQPCQD IPEEVNQPCQ QVSDICQPCE ENHDDVDQTC QEVPQISQPC
1310 1320 1330 1340 1350
EDASQLYQKV SKEVCELCQN SEEVNQPCQG VPVEIGRLVH GFPVGVGGLA
1360 1370 1380 1390 1400
QEVLGEVGRP TQEIPEELSQ SCQEFSVDIG RLAQEASAIN LLSPDTPEVD
1410 1420 1430 1440 1450
NPPLEFPGEG ALQAQEVSEW VKQQQSYVVP ELIDQLSREE VPQVQCPPSN
1460 1470
ANPQSQSLAP DQNAPLPPAT CDSSFSH
Length:1,477
Mass (Da):163,665
Last modified:October 23, 2007 - v1
Checksum:iDF692A0B30BB1EB4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC153248 mRNA. Translation: AAI53249.1.
RefSeqiNP_001098824.1. NM_001105354.1.
XP_005222243.1. XM_005222186.3.
UniGeneiBt.27184.

Genome annotation databases

EnsembliENSBTAT00000018635; ENSBTAP00000018635; ENSBTAG00000014005.
GeneIDi510684.
KEGGibta:510684.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC153248 mRNA. Translation: AAI53249.1.
RefSeqiNP_001098824.1. NM_001105354.1.
XP_005222243.1. XM_005222186.3.
UniGeneiBt.27184.

3D structure databases

ProteinModelPortaliA7Z050.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000018635.

Proteomic databases

PaxDbiA7Z050.
PRIDEiA7Z050.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000018635; ENSBTAP00000018635; ENSBTAG00000014005.
GeneIDi510684.
KEGGibta:510684.

Organism-specific databases

CTDi9677.

Phylogenomic databases

eggNOGiKOG1057. Eukaryota.
ENOG410XNSN. LUCA.
GeneTreeiENSGT00390000009048.
HOGENOMiHOG000177917.
HOVERGENiHBG108657.
InParanoidiA7Z050.
KOiK13024.
OMAiMHSNQAS.
OrthoDBiEOG091G00ZU.
TreeFamiTF313594.

Enzyme and pathway databases

ReactomeiR-BTA-1855167. Synthesis of pyrophosphates in the cytosol.

Gene expression databases

BgeeiENSBTAG00000014005.

Family and domain databases

CDDicd07061. HP_HAP_like. 1 hit.
Gene3Di3.40.50.1240. 4 hits.
InterProiIPR033379. Acid_Pase_AS.
IPR000560. His_Pase_clade-2.
IPR029033. His_PPase_superfam.
IPR030138. PPIP5K1.
[Graphical view]
PANTHERiPTHR12750:SF11. PTHR12750:SF11. 1 hit.
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 3 hits.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVIP1_BOVIN
AccessioniPrimary (citable) accession number: A7Z050
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 23, 2007
Last modified: November 2, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.