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A7Z050

- VIP1_BOVIN

UniProt

A7Z050 - VIP1_BOVIN

Protein

Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1

Gene

PPIP5K1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (23 Oct 2007)
      Previous versions | rss
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    Functioni

    Bifunctional inositol kinase that acts in concert with the IP6K kinases IP6K1, IP6K2 and IP6K3 to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, exocytosis, insulin signaling and neutrophil activation. Phosphorylates inositol hexakisphosphate (InsP6) at positions 1 or 3 to produce PP-InsP5 which is in turn phosphorylated by IP6Ks to produce (PP)2-InsP4. Alternatively, phosphorylates at position 1 or 3 PP-InsP5, produced by IP6Ks from InsP6, to produce (PP)2-InsP4. Activated when cells are exposed to hyperosmotic stress By similarity.By similarity

    Catalytic activityi

    ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate.
    ATP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.
    ATP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.
    ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei145 – 1451ATPBy similarity
    Binding sitei198 – 1981ATPBy similarity
    Binding sitei205 – 2051ATPBy similarity
    Binding sitei224 – 2241ATPBy similarity
    Binding sitei259 – 2591SubstrateBy similarity
    Binding sitei273 – 2731SubstrateBy similarity
    Binding sitei275 – 2751ATPBy similarity
    Binding sitei320 – 3201ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi248 – 2514ATPBy similarity
    Nucleotide bindingi257 – 2593ATPBy similarity
    Nucleotide bindingi332 – 3343ATPBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. diphosphoinositol-pentakisphosphate kinase activity Source: UniProtKB
    4. inositol-1,3,4,5,6-pentakisphosphate kinase activity Source: UniProtKB
    5. inositol hexakisphosphate 1-kinase activity Source: UniProtKB-EC
    6. inositol hexakisphosphate 3-kinase activity Source: UniProtKB-EC
    7. inositol hexakisphosphate 5-kinase activity Source: UniProtKB

    GO - Biological processi

    1. inositol metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_218731. Synthesis of pyrophosphates in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1 (EC:2.7.4.21, EC:2.7.4.24)
    Alternative name(s):
    Diphosphoinositol pentakisphosphate kinase 1
    Histidine acid phosphatase domain-containing protein 2A
    InsP6 and PP-IP5 kinase 1
    Gene namesi
    Name:PPIP5K1
    Synonyms:HISPPD2A, VIP1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 21

    Subcellular locationi

    Cytoplasmcytosol By similarity. Cell membrane By similarity
    Note: Relocalizes to the plasma membrane upon activation of the PtdIns 3-kinase pathway.By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. nucleus Source: Ensembl
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 14771477Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1PRO_0000315687Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1148 – 11481PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiA7Z050.

    Interactioni

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000018635.

    Structurei

    3D structure databases

    ProteinModelPortaliA7Z050.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni64 – 652Substrate bindingBy similarity
    Regioni224 – 2252Substrate bindingBy similarity
    Regioni337 – 3404Substrate bindingBy similarity
    Regioni382 – 45372Polyphosphoinositide-binding domainBy similarityAdd
    BLAST

    Domaini

    The polyphosphoinositide-binding domain mediates binding of PtdIns(3,4,5)P3 and InsP6. Despite its similarity with the phosphatase domain of histidine acid phosphatases, it has no phosphatase activity By similarity.By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG245915.
    GeneTreeiENSGT00390000009048.
    HOGENOMiHOG000177917.
    HOVERGENiHBG108657.
    InParanoidiA7Z050.
    KOiK13024.
    OMAiEEVSQPC.
    OrthoDBiEOG77M8MT.
    TreeFamiTF313594.

    Family and domain databases

    Gene3Di3.40.50.1240. 4 hits.
    InterProiIPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    [Graphical view]
    PfamiPF00328. His_Phos_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53254. SSF53254. 3 hits.
    PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7Z050-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWSLPASEGE SATAHFFLGA GDEGLGTRGL GMRPEESDSE LLEDEEDEVP     50
    PEPQIIVGIC AMTKKSKSKP MTQILERLCR FDYLTVIILG EDVILNEPVE 100
    NWPSCHCLIS FHSKGFPLDK AVAYSKLRNP FLINDLAMQY YIQDRREVYR 150
    ILQEEGIDLP RYAVLNRDPA RPEECNLIEG EDQVEVNGAV FPKPFVEKPV 200
    SAEDHNVYIY YPSSAGGGSQ RLFRKIGSRS SVYSPESSVR KTGSYIYEEF 250
    MPTDGTDVKV YTVGPDYAHA EARKSPALDG KVERDSEGKE IRYPVMLTAM 300
    EKLVARKVCV AFKQTVCGFD LLRANGHSFV CDVNGFSFVK NSMKYYDDCA 350
    KILGNTIMRE LAPQFQIPWS IPMEAEDIPI VPTTSGTMME LRCVIAIIRH 400
    GDRTPKQKMK MEVTHPRFFS LFEKHGGYKT GKLKLKRPEQ LQEVLDITRL 450
    LLAELEKEPG GEIEEKTGKL EQLKSVLEMY GHFSGINRKV QLTYYPHGVK 500
    ASNEGQDTQR EALAPSLLLV LKWGGELTPA GRVQAEELGR AFRCMYPGGQ 550
    GDYAGFPGCG LLRLHSTFRH DLKIYASDEG RVQMTAAAFA KGLLALEGEL 600
    TPILVQMVKS ANMNGLLDSD GDSLSSCQHR VKARLHHILQ QDAPFGPEDY 650
    NQLAPTGSTS LLSSMAVIQN PVKVCDQVFD LIENLTHQIR ERMQDPKSVD 700
    LQLYHSETLE LMLQRWSKLE RDFRQKSGRY DISKIPDIYD CVKYDVQHNG 750
    SLGLQGTAEL LRLSKALADV VIPQEYGISR EEKLEIAVGF CLPLLRKILL 800
    DLQRTHEDES VNKLHPLYSR GVLSPGRHVR TRLYFTSESH VHSLLSVFRY 850
    GGLLDETKDT QWQRALAYLS AISELNYMTQ IVIMLYEDNT RDPLSEERFH 900
    VELHFSPGVK GVEEEGSAPT GCGFRPASSE NEERKADQGS VEDLCPGKAS 950
    DEPDRALQTS PLPSEGPGLP KRSPLIRNRK AGSMEVLSET SSSRPGGHRL 1000
    FSSSRPPTEM KQSGLGSQCT GLFSTTVLGG SSSAPNLQDY ARSQGKKLPP 1050
    ASLKHRDELL FVPAVKRFSV SFAKHPTNGF EGCSMVPTIY PLETLHNALS 1100
    LRQVSEFLSR VCQRHTEAQA QASAALFDSM HSNQASDSPF SPPRTLHSPT 1150
    LQLQQRSEKP PWYSSGPSST VSSAGPSSPT AVDGNCPFGF SDQPSVSSHV 1200
    TEEYQGLGLL QEAPGSGAQE PPLEGQQEPF EQNQSPQEPP VETKKPCQEV 1250
    AEEVSQPCQD IPEEVNQPCQ QVSDICQPCE ENHDDVDQTC QEVPQISQPC 1300
    EDASQLYQKV SKEVCELCQN SEEVNQPCQG VPVEIGRLVH GFPVGVGGLA 1350
    QEVLGEVGRP TQEIPEELSQ SCQEFSVDIG RLAQEASAIN LLSPDTPEVD 1400
    NPPLEFPGEG ALQAQEVSEW VKQQQSYVVP ELIDQLSREE VPQVQCPPSN 1450
    ANPQSQSLAP DQNAPLPPAT CDSSFSH 1477
    Length:1,477
    Mass (Da):163,665
    Last modified:October 23, 2007 - v1
    Checksum:iDF692A0B30BB1EB4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC153248 mRNA. Translation: AAI53249.1.
    RefSeqiNP_001098824.1. NM_001105354.1.
    XP_005222243.1. XM_005222186.1.
    UniGeneiBt.27184.

    Genome annotation databases

    EnsembliENSBTAT00000018635; ENSBTAP00000018635; ENSBTAG00000014005.
    GeneIDi510684.
    KEGGibta:510684.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC153248 mRNA. Translation: AAI53249.1 .
    RefSeqi NP_001098824.1. NM_001105354.1.
    XP_005222243.1. XM_005222186.1.
    UniGenei Bt.27184.

    3D structure databases

    ProteinModelPortali A7Z050.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000018635.

    Proteomic databases

    PRIDEi A7Z050.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000018635 ; ENSBTAP00000018635 ; ENSBTAG00000014005 .
    GeneIDi 510684.
    KEGGi bta:510684.

    Organism-specific databases

    CTDi 9677.

    Phylogenomic databases

    eggNOGi NOG245915.
    GeneTreei ENSGT00390000009048.
    HOGENOMi HOG000177917.
    HOVERGENi HBG108657.
    InParanoidi A7Z050.
    KOi K13024.
    OMAi EEVSQPC.
    OrthoDBi EOG77M8MT.
    TreeFami TF313594.

    Enzyme and pathway databases

    Reactomei REACT_218731. Synthesis of pyrophosphates in the cytosol.

    Miscellaneous databases

    NextBioi 20869569.

    Family and domain databases

    Gene3Di 3.40.50.1240. 4 hits.
    InterProi IPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    [Graphical view ]
    Pfami PF00328. His_Phos_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53254. SSF53254. 3 hits.
    PROSITEi PS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Brain cortex.

    Entry informationi

    Entry nameiVIP1_BOVIN
    AccessioniPrimary (citable) accession number: A7Z050
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: October 23, 2007
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3