Reviewed, UniProtKB/Swiss-Prot A7YWE4 (AL4A1_BOVIN)

Names and origin

Protein names

Recommended name:
    Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
      Short name=P5C dehydrogenase
    EC=1.5.1.12
Alternative name(s):
    Aldehyde dehydrogenase family 4 member A1

Gene names

Name:

ALDH4A1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	563 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes By similarity.
Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)(+) + 2 H(2)O = L-glutamate + NAD(P)H.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion matrixBy similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Proline metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: InterPro
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 24	24	Mitochondrion By similarity
	Chain	25 – 563	539	Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial		PRO_0000342182
Regions
	Nucleotide binding	296 – 301	6	NAD By similarity
Sites
	Active site	314	1	Proton acceptor By similarity
	Active site	348	1	Nucleophile By similarity
	Site	211	1	Transition state stabilizer By similarity
Amino acid modifications
	Modified residue	99	1	N6-acetyllysine By similarity
	Modified residue	114	1	N6-acetyllysine By similarity
	Modified residue	402	1	N6-acetyllysine By similarity
	Modified residue	505	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7YWE4-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: E45B568B4844FEF0	FASTA	563	61,495
	10 20 30 40 50 60 MLLRSAALCR ALLARRGRAA GLCRRCVSSL QVANEPVLAF TQGSPERDAL QKALKELKGR 70 80 90 100 110 120 TEAIPCVVGD EEVWTSDVRY QASPFNHGHK VAKFCYADKA LLHRAIGAAL AARKEWDLKP 130 140 150 160 170 180 VADRAQVFLK AADLLSGPRR AEVLAKTMVG QGKTVIQAEI DAAAELIDFF RFNAKFAMEL 190 200 210 220 230 240 EGEQPLSVPP STNSMLYRGL EGFVAAISPF NFTAIGGNLA GAPALMGNVV LWKPSDTAML 250 260 270 280 290 300 ASYAVYRILR EAGLPPNIIQ FVPADGPTFG DTVTSSEHLC GINFTGSVPT FKHLWKQVAQ 310 320 330 340 350 360 NLDRFRTFPR LAGECGGKNF HFVHRSADVD SVVSGTLRSA FEYGGQKCSA CSRLYAPRSL 370 380 390 400 410 420 WPQIKGRLLE ELGGIKVGNP AEDFGTFFSA VIDAKSFGRI RKWLEHARSS PSLTILAGGH 430 440 450 460 470 480 CDDSVGYFVE PCIVETKDPQ DPIMKEEIFG PVLAVYVYPD EEYKETLRLV DSTTSYGLTG 490 500 510 520 530 540 AVFAQDKDVL REATELLRHA AGNFYINDKS TGSVVGQQPF GGARASGTND KPGGPHYVLR 550 560 WTSPQVIKET HGPLGDWRYP YMQ

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Ascending colon.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC134524 mRNA. Translation: AAI34525.1.
RefSeq	NP_001099116.1.
UniGene	Bt.3248
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000030335. Bos taurus. [Contig view]
GeneID	100126042.
KEGG	bta:100126042.
Family and domain databases
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. IPR005931. d-1-pyrroline-5-COlate_DHase-1. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01236. D1pyr5carbox1. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AL4A1_BOVIN

Accession

Primary (citable) accession number: A7YWE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	October 23, 2007
Last modified:	November 25, 2008
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)