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Reviewed, UniProtKB/Swiss-Prot A7YWE4 (AL4A1_BOVIN)

Last modified November 25, 2008. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
      Short name=P5C dehydrogenase
    EC=1.5.1.12
Alternative name(s):
    Aldehyde dehydrogenase family 4 member A1
Gene names
Name: ALDH4A1
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length563 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes By similarity.

Catalytic activity

(S)-1-pyrroline-5-carboxylate + NAD(P)(+) + 2 H(2)O = L-glutamate + NAD(P)H.

Pathway

Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion matrixBy similarity.

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords

   Biological processProline metabolism
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandNAD
   Molecular functionOxidoreductase
   PTMAcetylation
Phosphoprotein

Gene Ontology (GO)

   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

proline biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentmitochondrial matrix

Inferred from electronic annotation. Source: InterPro

   Molecular function1-pyrroline-5-carboxylate dehydrogenase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2424Mitochondrion By similarity
Chain25 – 563539Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
PRO_0000342182

Regions

Nucleotide binding296 – 3016NAD By similarity

Sites

Active site3141Proton acceptor By similarity
Active site3481Nucleophile By similarity
Site2111Transition state stabilizer By similarity

Amino acid modifications

Modified residue991N6-acetyllysine By similarity
Modified residue1141N6-acetyllysine By similarity
Modified residue4021N6-acetyllysine By similarity
Modified residue5051Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
A7YWE4-1 [UniParc].

Last modified October 23, 2007. Version 1.
Checksum: E45B568B4844FEF0

FASTA56361,495
        10         20         30         40         50         60 
MLLRSAALCR ALLARRGRAA GLCRRCVSSL QVANEPVLAF TQGSPERDAL QKALKELKGR 

        70         80         90        100        110        120 
TEAIPCVVGD EEVWTSDVRY QASPFNHGHK VAKFCYADKA LLHRAIGAAL AARKEWDLKP 

       130        140        150        160        170        180 
VADRAQVFLK AADLLSGPRR AEVLAKTMVG QGKTVIQAEI DAAAELIDFF RFNAKFAMEL 

       190        200        210        220        230        240 
EGEQPLSVPP STNSMLYRGL EGFVAAISPF NFTAIGGNLA GAPALMGNVV LWKPSDTAML 

       250        260        270        280        290        300 
ASYAVYRILR EAGLPPNIIQ FVPADGPTFG DTVTSSEHLC GINFTGSVPT FKHLWKQVAQ 

       310        320        330        340        350        360 
NLDRFRTFPR LAGECGGKNF HFVHRSADVD SVVSGTLRSA FEYGGQKCSA CSRLYAPRSL 

       370        380        390        400        410        420 
WPQIKGRLLE ELGGIKVGNP AEDFGTFFSA VIDAKSFGRI RKWLEHARSS PSLTILAGGH 

       430        440        450        460        470        480 
CDDSVGYFVE PCIVETKDPQ DPIMKEEIFG PVLAVYVYPD EEYKETLRLV DSTTSYGLTG 

       490        500        510        520        530        540 
AVFAQDKDVL REATELLRHA AGNFYINDKS TGSVVGQQPF GGARASGTND KPGGPHYVLR 

       550        560 
WTSPQVIKET HGPLGDWRYP YMQ 

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Ascending colon.

Cross-references

Sequence databases

BC134524 mRNA. Translation: AAI34525.1.
RefSeqNP_001099116.1.
UniGeneBt.3248

3D structure databases

ModBaseSearch...

Genome annotation databases

EnsemblENSBTAG00000030335. Bos taurus. [Contig view]
GeneID100126042.
KEGGbta:100126042.

Family and domain databases

InterProIPR016160. Ald_DHase_CS.
IPR016162. Ald_DHase_N.
IPR015590. Aldehyde_DHase.
IPR005931. d-1-pyrroline-5-COlate_DHase-1.
[Graphical view]
Gene3DG3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHERPTHR11699. Aldehyde_dehyd. 1 hit.
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
TIGRFAMsTIGR01236. D1pyr5carbox1. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAL4A1_BOVIN
AccessionPrimary (citable) accession number: A7YWE4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: October 23, 2007
Last modified: November 25, 2008
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents