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Protein

Arginine--tRNA ligase, cytoplasmic

Gene

RARS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.By similarity

Catalytic activityi

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei211L-arginineBy similarity1
Binding sitei384L-arginineBy similarity1
Binding sitei388L-arginineBy similarity1
Binding sitei412L-arginineBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine--tRNA ligase, cytoplasmic (EC:6.1.1.19By similarity)
Alternative name(s):
Arginyl-tRNA synthetase
Short name:
ArgRSBy similarity
Gene namesi
Name:RARSImported
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Organism-specific databases

VGNCiVGNC:33733 RARS

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003954331 – 660Arginine--tRNA ligase, cytoplasmicAdd BLAST660

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiA7YW98
PeptideAtlasiA7YW98
PRIDEiA7YW98

Expressioni

Gene expression databases

BgeeiENSBTAG00000014626

Interactioni

Subunit structurei

Interacts (via N-terminus) with AIMP1 (via N-terminus); this stimulates its catalytic activity. Interacts (via N-terminus) with LARS2 (via C-terminus). Monomer. Part of a multisubunit complex that groups tRNA ligases for Arg (RARS), Asp (DARS), Gln (QARS), Ile (IARS), Leu (LARS), Lys (KARS), Met (MARS) the bifunctional ligase for Glu and Pro (EPRS) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18. Interacts with QARS. Part of a complex composed of RARS, QARS and AIMP1.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019475

Structurei

3D structure databases

ProteinModelPortaliA7YW98
SMRiA7YW98
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 72Could be involved in the assembly of the multisynthetase complexBy similarityAdd BLAST72
Regioni200 – 202L-arginine bindingBy similarity3
Regioni529 – 543Interaction with tRNABy similarityAdd BLAST15

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi201 – 212"HIGH" regionBy similarityAdd BLAST12

Domaini

The alpha-helical N-terminus (residues 1-72) mediates interaction with AIMP1 and thereby contributes to the assembly of the multisynthetase complex.By similarity

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.Sequence analysis

Phylogenomic databases

eggNOGiKOG1195 Eukaryota
COG0018 LUCA
GeneTreeiENSGT00530000063407
HOGENOMiHOG000247212
HOVERGENiHBG029238
InParanoidiA7YW98
KOiK01887
OMAiNKPLHLG
OrthoDBiEOG091G03A6
TreeFamiTF106111

Family and domain databases

CDDicd00671 ArgRS_core, 1 hit
Gene3Di3.30.1360.70, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00123 Arg_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR001278 Arg-tRNA-ligase
IPR005148 Arg-tRNA-synth_N
IPR036695 Arg-tRNA-synth_N_sf
IPR035684 ArgRS_core
IPR008909 DALR_anticod-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
PANTHERiPTHR11956 PTHR11956, 1 hit
PfamiView protein in Pfam
PF03485 Arg_tRNA_synt_N, 1 hit
PF05746 DALR_1, 1 hit
PF00750 tRNA-synt_1d, 1 hit
PRINTSiPR01038 TRNASYNTHARG
SMARTiView protein in SMART
SM01016 Arg_tRNA_synt_N, 1 hit
SM00836 DALR_1, 1 hit
SUPFAMiSSF47323 SSF47323, 1 hit
SSF55190 SSF55190, 1 hit
TIGRFAMsiTIGR00456 argS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A7YW98-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDALVAHCSA RLLQQEKEIK FLTAEVDRLK NYSCSEASAD LEKLREENLK
60 70 80 90 100
LKYRLNILRK SLQAERNKPT KTMININSCL EEVFGCAIKA AYPVLENPPL
110 120 130 140 150
IVTPSQQPKF GDYQCNSAMG ICQMLKTKEQ KVNPREIAEN IVKHLPDNEY
160 170 180 190 200
IEKVEIAGPG FINIHLRKGF VSQQLTNLLV NGVKIPSIGE NKKVIVDFSS
210 220 230 240 250
PNIAKEMHVG HLRSTIIGES MCRLFEFAGY NVLRLNHVGD WGTQFGMLIA
260 270 280 290 300
HLQDKFPDYL TVSPPIGDLQ AFYKESKKRF DTEEEFKKRA YQCVVLLQSK
310 320 330 340 350
NPDIIKAWKL ICDVSRQEFN KIYEALDISL IERGESFYQD RMHDIVKEFE
360 370 380 390 400
DRGFVQVDDG RKIVFVPGCS VPLTIVKSDG GYTYDTSDLA AIKQRLFEEK
410 420 430 440 450
ADMIIYVVDN GQSLHFQTVF GAAQMIGWYD PAVTRVSHAG FGVVLGEDKK
460 470 480 490 500
KFKTRSGETV RLIDLLEEGL KRSMDKLKEK ERDKVLTTEE LKAAQTSVAY
510 520 530 540 550
GCIKYADLSH NRLNDYIFSF DKMLDDRGNT AAYLLYAFTR IRSIARLANI
560 570 580 590 600
DEEMLRKAAH ETEIILDHEK EWKLGRCILR FPEVLQKILD DLLLHTLCDY
610 620 630 640 650
IYELATTFTE FYDSCYCVEK DRQSGEVLKV NMWRMLLCEA VAAVMAKGFD
660
ILGIKPVQRM
Length:660
Mass (Da):75,561
Last modified:October 23, 2007 - v1
Checksum:iEA5DCB46414C81C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC134468 mRNA Translation: AAI34469.1
RefSeqiNP_001098808.1, NM_001105338.2
UniGeneiBt.22038

Genome annotation databases

EnsembliENSBTAT00000019475; ENSBTAP00000019475; ENSBTAG00000014626
GeneIDi506305
KEGGibta:506305

Similar proteinsi

Entry informationi

Entry nameiSYRC_BOVIN
AccessioniPrimary (citable) accession number: A7YW98
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: October 23, 2007
Last modified: May 23, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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