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A7XQ02 (MLX56_MORAL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mulatexin

Short name=MLX56
Alternative name(s):
Latex protein
OrganismMorus alba (White mulberry)
Taxonomic identifier3498 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesMoraceaeMorus

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Chitin-binding protein which slows larval growth when consumed by the lepidopteran species S.ricini and M.brassica, but not when consumed by the mulberry specialist B.mori. Lacks chitinase activity. Ref.1

Subcellular location

Secreted Ref.1.

Post-translational modification

Glycosylated. Ref.1

Miscellaneous

On the 2D-gel the determined MW of this protein is: 56 kDa. Ref.1

Sequence similarities

Contains 2 chitin-binding type-1 domains.

Ontologies

Keywords
   Biological processPlant defense
   Cellular componentSecreted
   DomainRepeat
Signal
   LigandChitin-binding
   PTMDisulfide bond
Glycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcell wall macromolecule catabolic process

Inferred from electronic annotation. Source: InterPro

defense response to insect

Inferred from direct assay Ref.1. Source: UniProtKB

   Cellular_componentextracellular region

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functionchitin binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential Ref.1
Chain22 – 415394Mulatexin Ref.1
PRO_0000401103

Regions

Domain23 – 6644Chitin-binding type-1 1
Domain125 – 16743Chitin-binding type-1 2
Compositional bias68 – 13972Pro-rich

Amino acid modifications

Glycosylation2641N-linked (GlcNAc...) Potential
Disulfide bond26 ↔ 41 By similarity UniProtKB P11218
Disulfide bond35 ↔ 47 By similarity UniProtKB P11218
Disulfide bond40 ↔ 54 By similarity UniProtKB P11218
Disulfide bond60 ↔ 64 By similarity UniProtKB P11218
Disulfide bond128 ↔ 143 By similarity UniProtKB P11218
Disulfide bond137 ↔ 149 By similarity UniProtKB P11218
Disulfide bond142 ↔ 156 By similarity UniProtKB P11218
Disulfide bond161 ↔ 165 By similarity UniProtKB P11218

Sequences

Sequence LengthMass (Da)Tools
A7XQ02 [UniParc].

Last modified April 29, 2008. Version 2.
Checksum: 3555EA15320D6457

FASTA41545,148
        10         20         30         40         50         60 
MKFRTLLIIF SLVFLLEIVS ASEPQCGRDA GGALCHGNLC CSHWGFCGTT AIYCDVDQGC 

        70         80         90        100        110        120 
QSQCWSSPPP PSPPPPPPSP PPPSPPPPSP PPPSPPPPSP PPPSPPPPSP PPPSPPPPGG 

       130        140        150        160        170        180 
PERPDHRCGR ALGNPPCNPG RCCSIHNWCG STAAYCRGSS CQYQCWNSLL SALISNGNNA 

       190        200        210        220        230        240 
ISKIISKSVF DEMFKHMKDC PSKGFYSYDA FIIATTSFPH FGTTGDITTR KRELAAFFAQ 

       250        260        270        280        290        300 
TSLATTGQRF DSQDLYVWGY CHINETTNGN DNDYCTSAHW PCPSGKKYNS RGAVQLTHNY 

       310        320        330        340        350        360 
NYGLAGEALG LDLINNPDLV ATDPVISFKT AIWFWMAQHD NKLSCHDILI NANSGYVIGN 

       370        380        390        400        410 
IIKNSGYQNG LITNTISTMR GIGYYKRYCD MLGVSYGDNL DSWYDQTHFS EVARM 

« Hide

References

[1]"A unique latex protein, MLX56, defends mulberry trees from insects."
Wasano N., Konno K., Nakamura M., Hirayama C., Hattori M., Tateishi K.
Phytochemistry 70:880-888(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-30, FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, GLYCOSYLATION.
Strain: cv. Shin-ichinose.
Tissue: Latex.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF535852 mRNA. Translation: ABS86614.2.

3D structure databases

ProteinModelPortalA7XQ02.
SMRA7XQ02. Positions 122-168, 181-410.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.60.10. 2 hits.
InterProIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamPF00187. Chitin_bind_1. 2 hits.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
ProDomPD000609. Chitin_bd_1. 2 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00270. ChtBD1. 2 hits.
[Graphical view]
SUPFAMSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 2 hits.
PROSITEPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 2 hits.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMLX56_MORAL
AccessionPrimary (citable) accession number: A7XQ02
Entry history
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: April 29, 2008
Last modified: February 19, 2014
This is version 27 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families