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Protein

Hydroxyproline O-galactosyltransferase GALT2

Gene

GALT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possesses hydroxyproline O-galactosyltransferase activity. Transfers galactose from UDP-galactose to hydroxyproline residues in the arabinogalactan proteins (AGPs). Is specific for AGPs containing non-contiguous peptidyl hydroxyproline residues. Utilizes UDP-galactose solely as sugar donor. The addition of galactose onto the peptidyl hydroxyproline residues in AGP core proteins represents the first committed step in arabinogalactan polysaccharide addition. AGP glycans play essential roles in both vegetative and reproductive plant growth.3 Publications

Cofactori

Mn2+1 Publication

pH dependencei

Optimum pH is 6.5.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese

Enzyme and pathway databases

BioCyciARA:AT4G21060-MONOMER.
ARA:GQT-1147-MONOMER.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyproline O-galactosyltransferase GALT21 Publication (EC:2.4.1.-1 Publication)
Short name:
AtGALT21 Publication
Alternative name(s):
Beta-1,3-galactosyltransferase 20Curated
Gene namesi
Name:GALT21 Publication
Synonyms:B3GALT201 Publication
Ordered Locus Names:At4g21060
ORF Names:T13K14.220
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G21060.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222CytoplasmicCuratedAdd
BLAST
Transmembranei23 – 4321Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini44 – 684641LumenalCuratedAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced levels of arabinogalactan proteins (PubMed:26690932, PubMed:25974423). Defects in root hair growth, root elongation, pollen tube growth, flowering time, leaf development, silique length and inflorescence growth. Increased sensitivity to salt stress (PubMed:25974423).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 684684Hydroxyproline O-galactosyltransferase GALT2PRO_0000359430Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence analysis
Glycosylationi127 – 1271N-linked (GlcNAc...)Sequence analysis
Glycosylationi162 – 1621N-linked (GlcNAc...)Sequence analysis
Glycosylationi524 – 5241N-linked (GlcNAc...)Sequence analysis
Glycosylationi632 – 6321N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiA7XDQ9.

Expressioni

Tissue specificityi

Expressed in stems and at lower levels in cauline leaves and siliques.1 Publication

Inductioni

By salt stress.1 Publication

Gene expression databases

ExpressionAtlasiA7XDQ9. baseline and differential.
GenevisibleiA7XDQ9. AT.

Interactioni

Protein-protein interaction databases

BioGridi13144. 4 interactions.
STRINGi3702.AT4G21060.1.

Structurei

3D structure databases

ProteinModelPortaliA7XDQ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini191 – 405215GalectinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated
Contains 1 galectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2287. Eukaryota.
ENOG410ZZ1B. LUCA.
HOGENOMiHOG000239189.
InParanoidiA7XDQ9.
PhylomeDBiA7XDQ9.

Family and domain databases

Gene3Di2.60.120.200. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001079. Galectin_CRD.
IPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF00337. Gal-bind_lectin. 1 hit.
PF01762. Galactosyl_T. 1 hit.
[Graphical view]
SMARTiSM00908. Gal-bind_lectin. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiPS51304. GALECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: A7XDQ9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKRVKSESFR GVYSSRRFKL SHFLLAIAGF YLVFLAFKFP HFIEMVAMLS
60 70 80 90 100
GDTGLDGALS DTSLDVSLSG SLRNDMLNRK LEDEDHQSGP STTQKVSPEE
110 120 130 140 150
KINGSKQIQP LLFRYGRISG EVMRRRNRTI HMSPFERMAD EAWILGSKAW
160 170 180 190 200
EDVDKFEVDK INESASIFEG KVESCPSQIS MNGDDLNKAN RIMLLPCGLA
210 220 230 240 250
AGSSITILGT PQYAHKESVP QRSRLTRSYG MVLVSQFMVE LQGLKTGDGE
260 270 280 290 300
YPPKILHLNP RIKGDWNHRP VIEHNTCYRM QWGVAQRCDG TPSKKDADVL
310 320 330 340 350
VDGFRRCEKW TQNDIIDMVD SKESKTTSWF KRFIGREQKP EVTWSFPFAE
360 370 380 390 400
GKVFVLTLRA GIDGFHINVG GRHVSSFPYR PGFTIEDATG LAVTGDVDIH
410 420 430 440 450
SIHATSLSTS HPSFSPQKAI EFSSEWKAPP LPGTPFRLFM GVLSATNHFS
460 470 480 490 500
ERMAVRKTWM QHPSIKSSDV VARFFVALNP RKEVNAMLKK EAEYFGDIVI
510 520 530 540 550
LPFMDRYELV VLKTIAICEF GVQNVTAPYI MKCDDDTFIR VESILKQIDG
560 570 580 590 600
VSPEKSLYMG NLNLRHRPLR TGKWTVTWEE WPEAVYPPYA NGPGYIISSN
610 620 630 640 650
IAKYIVSQNS RHKLRLFKME DVSMGLWVEQ FNASMQPVEY SHSWKFCQYG
660 670 680
CTLNYYTAHY QSPSQMMCLW DNLLKGRPQC CNFR
Length:684
Mass (Da):77,912
Last modified:October 23, 2007 - v1
Checksum:iC877E82BDC30326A
GO

Sequence cautioni

The sequence CAB45901.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB79106.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF428438 mRNA. Translation: ABR58857.1.
AL080282 Genomic DNA. Translation: CAB45901.1. Sequence problems.
AL161554 Genomic DNA. Translation: CAB79106.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84393.1.
PIRiT10648.
RefSeqiNP_001154260.1. NM_001160788.1. [A7XDQ9-1]
UniGeneiAt.32667.

Genome annotation databases

EnsemblPlantsiAT4G21060.2; AT4G21060.2; AT4G21060. [A7XDQ9-1]
GeneIDi827853.
KEGGiath:AT4G21060.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF428438 mRNA. Translation: ABR58857.1.
AL080282 Genomic DNA. Translation: CAB45901.1. Sequence problems.
AL161554 Genomic DNA. Translation: CAB79106.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84393.1.
PIRiT10648.
RefSeqiNP_001154260.1. NM_001160788.1. [A7XDQ9-1]
UniGeneiAt.32667.

3D structure databases

ProteinModelPortaliA7XDQ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13144. 4 interactions.
STRINGi3702.AT4G21060.1.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Proteomic databases

PaxDbiA7XDQ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G21060.2; AT4G21060.2; AT4G21060. [A7XDQ9-1]
GeneIDi827853.
KEGGiath:AT4G21060.

Organism-specific databases

TAIRiAT4G21060.

Phylogenomic databases

eggNOGiKOG2287. Eukaryota.
ENOG410ZZ1B. LUCA.
HOGENOMiHOG000239189.
InParanoidiA7XDQ9.
PhylomeDBiA7XDQ9.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciARA:AT4G21060-MONOMER.
ARA:GQT-1147-MONOMER.

Miscellaneous databases

PROiA7XDQ9.

Gene expression databases

ExpressionAtlasiA7XDQ9. baseline and differential.
GenevisibleiA7XDQ9. AT.

Family and domain databases

Gene3Di2.60.120.200. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001079. Galectin_CRD.
IPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF00337. Gal-bind_lectin. 1 hit.
PF01762. Galactosyl_T. 1 hit.
[Graphical view]
SMARTiSM00908. Gal-bind_lectin. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiPS51304. GALECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A unique beta-1,3-galactosyltransferase is indispensable for the biosynthesis of N-glycans containing Lewis a structures in Arabidopsis thaliana."
    Strasser R., Bondili J.S., Vavra U., Schoberer J., Svoboda B., Gloessl J., Leonard R., Stadlmann J., Altmann F., Steinkellner H., Mach L.
    Plant Cell 19:2278-2292(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
    Tissue: Leaf.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Identification of a novel group of putative Arabidopsis thaliana beta-(1,3)-galactosyltransferases."
    Qu Y., Egelund J., Gilson P.R., Houghton F., Gleeson P.A., Schultz C.J., Bacic A.
    Plant Mol. Biol. 68:43-59(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. "Functional identification of a hydroxyproline-o-galactosyltransferase specific for arabinogalactan protein biosynthesis in Arabidopsis."
    Basu D., Liang Y., Liu X., Himmeldirk K., Faik A., Kieliszewski M., Held M., Showalter A.M.
    J. Biol. Chem. 288:10132-10143(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
  6. "A small multigene hydroxyproline-O-galactosyltransferase family functions in arabinogalactan-protein glycosylation, growth and development in Arabidopsis."
    Basu D., Tian L., Wang W., Bobbs S., Herock H., Travers A., Showalter A.M.
    BMC Plant Biol. 15:295-295(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, COFACTOR, DISRUPTION PHENOTYPE.
  7. "Two hydroxyproline galactosyltransferases, GALT5 and GALT2, function in arabinogalactan-protein glycosylation, growth and development in Arabidopsis."
    Basu D., Wang W., Ma S., DeBrosse T., Poirier E., Emch K., Soukup E., Tian L., Showalter A.M.
    PLoS ONE 10:E0125624-E0125624(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  8. "Glycosylation of a fasciclin-like arabinogalactan-protein (SOS5) mediates root growth and seed mucilage adherence via a cell wall receptor-like kinase (FEI1/FEI2) pathway in Arabidopsis."
    Basu D., Tian L., Debrosse T., Poirier E., Emch K., Herock H., Travers A., Showalter A.M.
    PLoS ONE 11:E0145092-E0145092(2016) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY SALT STRESS.

Entry informationi

Entry nameiB3GTK_ARATH
AccessioniPrimary (citable) accession number: A7XDQ9
Secondary accession number(s): Q9SUA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: October 23, 2007
Last modified: May 11, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.