Reviewed, UniProtKB/Swiss-Prot A7XDQ9 (B3GTK_ARATH)

Names and origin

Protein names

Recommended name:
    Probable beta-1,3-galactosyltransferase 20
    EC=2.4.1.-

Gene names

Name:	B3GALT20
Ordered Locus Names:	At4g21060
ORF Names:	T13K14.220

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	684 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal glycosyl residue By similarity.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable.
Tissue specificity	Expressed in stems and at lower levels in cauline leaves and siliques. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 31 family. Contains 1 galectin domain.
Sequence caution	The sequence CAB45901.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB79106.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosyltransferase activity Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 684	684	Probable beta-1,3-galactosyltransferase 20		PRO_0000359430
Regions
	Transmembrane	23 – 43	21	Signal-anchor for type II membrane protein Potential
	Domain	191 – 405	215	Galectin
Amino acid modifications
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
	Glycosylation	162	1	N-linked (GlcNAc...) Potential
	Glycosylation	524	1	N-linked (GlcNAc...) Potential
	Glycosylation	632	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A7XDQ9-1 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: C877E82BDC30326A	FASTA	684	77,912
	10 20 30 40 50 60 MKRVKSESFR GVYSSRRFKL SHFLLAIAGF YLVFLAFKFP HFIEMVAMLS GDTGLDGALS 70 80 90 100 110 120 DTSLDVSLSG SLRNDMLNRK LEDEDHQSGP STTQKVSPEE KINGSKQIQP LLFRYGRISG 130 140 150 160 170 180 EVMRRRNRTI HMSPFERMAD EAWILGSKAW EDVDKFEVDK INESASIFEG KVESCPSQIS 190 200 210 220 230 240 MNGDDLNKAN RIMLLPCGLA AGSSITILGT PQYAHKESVP QRSRLTRSYG MVLVSQFMVE 250 260 270 280 290 300 LQGLKTGDGE YPPKILHLNP RIKGDWNHRP VIEHNTCYRM QWGVAQRCDG TPSKKDADVL 310 320 330 340 350 360 VDGFRRCEKW TQNDIIDMVD SKESKTTSWF KRFIGREQKP EVTWSFPFAE GKVFVLTLRA 370 380 390 400 410 420 GIDGFHINVG GRHVSSFPYR PGFTIEDATG LAVTGDVDIH SIHATSLSTS HPSFSPQKAI 430 440 450 460 470 480 EFSSEWKAPP LPGTPFRLFM GVLSATNHFS ERMAVRKTWM QHPSIKSSDV VARFFVALNP 490 500 510 520 530 540 RKEVNAMLKK EAEYFGDIVI LPFMDRYELV VLKTIAICEF GVQNVTAPYI MKCDDDTFIR 550 560 570 580 590 600 VESILKQIDG VSPEKSLYMG NLNLRHRPLR TGKWTVTWEE WPEAVYPPYA NGPGYIISSN 610 620 630 640 650 660 IAKYIVSQNS RHKLRLFKME DVSMGLWVEQ FNASMQPVEY SHSWKFCQYG CTLNYYTAHY 670 680 QSPSQMMCLW DNLLKGRPQC CNFR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A unique beta-1,3-galactosyltransferase is indispensable for the biosynthesis of N-glycans containing Lewis a structures in Arabidopsis thaliana." Strasser R., Bondili J.S., Vavra U., Schoberer J., Svoboda B., Gloessl J., Leonard R., Stadlmann J., Altmann F., Steinkellner H., Mach L. Plant Cell 19:2278-2292(2007) [PubMed: 17630273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: cv. Columbia. Tissue: Leaf.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Identification of a novel group of putative Arabidopsis thaliana beta-(1,3)-galactosyltransferases." Qu Y., Egelund J., Gilson P.R., Houghton F., Gleeson P.A., Schultz C.J., Bacic A. Plant Mol. Biol. 68:43-59(2008) [PubMed: 18548197] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	EF428438 mRNA. Translation: ABR58857.1. AL080282 Genomic DNA. Translation: CAB45901.1. Sequence problems. AL161554 Genomic DNA. Translation: CAB79106.1. Sequence problems.
IPI	IPI00529900.
PIR	T10648.
3D structure databases
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus AT4G21060 in contig CT486007_GR.
Organism-specific databases
TAIR	At4g21060.
Family and domain databases
InterPro	IPR013320. ConA-like_subgrp. IPR001079. Galectin_CRD. IPR002659. Glyco_trans_31. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF00337. Gal-bind_lectin. 1 hit. PF01762. Galactosyl_T. 1 hit. [Graphical view]
SMART	SM00276. GLECT. 1 hit. [Graphical view]
PROSITE	PS51304. GALECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3GTK_ARATH

Accession

Primary (citable) accession number: A7XDQ9
Secondary accession number(s): Q9SUA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	October 23, 2007
Last modified:	June 16, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)