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A7X8D2

- PRGR_COLGU

UniProt

A7X8D2 - PRGR_COLGU

Protein

Progesterone receptor

Gene

PGR

Organism
Colobus guereza (Mantled guereza) (Eastern black-and-white colobus monkey)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (23 Oct 2007)
      Previous versions | rss
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    Functioni

    The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor is involved in activation of c-SRC/MAPK signaling on hormone stimulation By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi568 – 64073Nuclear receptorPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri568 – 58821NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri604 – 62825NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: InterPro
    2. sequence-specific DNA binding transcription factor activity Source: InterPro
    3. steroid binding Source: UniProtKB-KW
    4. steroid hormone receptor activity Source: InterPro
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Lipid-binding, Metal-binding, Steroid-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Progesterone receptor
    Short name:
    PR
    Alternative name(s):
    Nuclear receptor subfamily 3 group C member 3
    Gene namesi
    Name:PGR
    Synonyms:NR3C3
    OrganismiColobus guereza (Mantled guereza) (Eastern black-and-white colobus monkey)
    Taxonomic identifieri33548 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeColobinaeColobus

    Subcellular locationi

    Nucleus. Cytoplasm
    Note: Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G1 and G2/M phases By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 934934Progesterone receptorPRO_0000375853Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki7 – 7Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Modified residuei20 – 201PhosphoserineBy similarity
    Modified residuei81 – 811PhosphoserineBy similarity
    Modified residuei130 – 1301PhosphoserineBy similarity
    Modified residuei162 – 1621PhosphoserineBy similarity
    Modified residuei190 – 1901PhosphoserineBy similarity
    Modified residuei213 – 2131PhosphoserineBy similarity
    Modified residuei294 – 2941Phosphoserine; by MAPK1By similarity
    Modified residuei345 – 3451Phosphoserine; by MAPKBy similarity
    Cross-linki388 – 388Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
    Cross-linki388 – 388Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
    Modified residuei400 – 4001Phosphoserine; by CDK2By similarity
    Cross-linki532 – 532Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei677 – 6771PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G2/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1 By similarity.By similarity
    Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294 By similarity.By similarity
    Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294 By similarity.By similarity
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation By similarity.By similarity

    Keywords - PTMi

    Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiA7X8D2.

    Interactioni

    Subunit structurei

    Interacts with SMARD1 and UNC45A. Interacts with CUEDC2; the interaction promotes ubiquitination, decreases sumoylation, and repesses transcriptional activity. Interacts with PIAS3; the interaction promotes sumoylation of PR in a hormone-dependent manner, inhibits DNA-binding, and alters nuclear export. Interacts with SP1; the interaction requires ligand-induced phosphorylation on Ser-345 by ERK1/2 MAPK. Interacts with PRMT2 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliA7X8D2.
    SMRiA7X8D2. Positions 564-641, 683-933.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 567567Modulating, Pro-RichAdd
    BLAST
    Regioni682 – 934253Steroid-bindingAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi183 – 1875Nuclear localization signalSequence Analysis

    Domaini

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

    Sequence similaritiesi

    Belongs to the nuclear hormone receptor family.Curated
    Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri568 – 58821NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri604 – 62825NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    Gene3Di1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR000128. Progest_rcpt.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view]
    PfamiPF00104. Hormone_recep. 1 hit.
    PF02161. Prog_receptor. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view]
    PRINTSiPR00544. PROGESTRONER.
    PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTiSM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view]
    SUPFAMiSSF48508. SSF48508. 2 hits.
    PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7X8D2-1 [UniParc]FASTAAdd to Basket

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    MTELKSKGPR APHVAGGPPS PEVGSPLLCR PAAGPFQGSQ TSDTLPEVSA    50
    IPISLDGLLF PRLCQGQDPP DKKTQNQQSL SDVEGAYSRA EATRGTGGSS 100
    SRPPEKDSGL LDSVLDTLLA PSGPGQSQPS PPACEVTSSW CLFGPELPED 150
    PPAAPATQRV LSPLMSRSGG KTEDSSGTAA AHKVLPRGLS PSRQLLLPTS 200
    GSPHWSGAPV KPSPQPTAVE VEEEDGSESE DSAGPLLKGK SRVLGGAAAG 250
    GGAAAVPPGA AAGGVGLVPK EDSRFSAPRV ALVEQDAPMA PGRSPLATTM 300
    MDFIHVPIVP LNHALLAART RQLLEDESYD GGAGAASAFA PPQSSPSASS 350
    TPVAVGDFPD CAYPPDAEPK DNAYPLYGDF QPLALKIKEE EEGAEASARS 400
    PGSYLVAGAN PAAFPDFPLG PPPQLPPRAP PSRPGEAAVT AAPASASVSS 450
    ASSPGSTLEC ILYKAEGALP QQGQFAPPPC KAPGAGGCLL PRDGLPSTSA 500
    SAAAAAGAAP TLYPALGLNG LPQLGYQAAV LKEGLQQVYP PYLNYLRPDS 550
    EASQSPQYSF ESLPQKICLI CGDEASGCHY GVLTCGSCKV FFKRAMEGQH 600
    NYLCAGRNDC IVDKIRRKNC PACRLRKCCQ AGMVLGGRKF KKFNKVRVMR 650
    ALDAVALPQP VGIPNESQVL SQRFTFSPGQ DIQLIPPLIK LLMSIEPDVI 700
    YAGHDNSKPD TSSSLLTSLN QLGERQLLSV VKWSKSLPGF RNLHIDDQIT 750
    LIQYSWMSLM VFGLGWRSYK HVSGQMLYFA PDLILNEQRM KESSFYSLCL 800
    TMWQIPQEFV KLQVSQEEFL CMKVLLLLNT IPLEGLRSQT QFEEMRSSYI 850
    RELIKAIGLR QKGVVSSSQR FYQLTKLLDN LHDLVKQLHL YCLNTFIQSR 900
    ALSVEFPEMM SEVIAAQLPK ILAGMVKPLL FHKK 934
    Length:934
    Mass (Da):99,320
    Last modified:October 23, 2007 - v1
    Checksum:i7E1A300E9CA44696
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ234988 Genomic DNA. Translation: ABB72148.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ234988 Genomic DNA. Translation: ABB72148.1 .

    3D structure databases

    ProteinModelPortali A7X8D2.
    SMRi A7X8D2. Positions 564-641, 683-933.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi A7X8D2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR000128. Progest_rcpt.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view ]
    Pfami PF00104. Hormone_recep. 1 hit.
    PF02161. Prog_receptor. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view ]
    PRINTSi PR00544. PROGESTRONER.
    PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTi SM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48508. SSF48508. 2 hits.
    PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The human progesterone receptor shows evidence of adaptive evolution associated with its ability to act as a transcription factor."
      Chen C., Opazo J.C., Erez O., Uddin M., Santolaya-Forgas J., Goodman M., Grossman L.I., Romero R., Wildman D.E.
      Mol. Phylogenet. Evol. 47:637-649(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiPRGR_COLGU
    AccessioniPrimary (citable) accession number: A7X8D2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 26, 2009
    Last sequence update: October 23, 2007
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3