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Protein

Progesterone receptor

Gene

PGR

Organism
Papio anubis (Olive baboon)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcriptional activator of several progesteron-dependent promoters in a variety of cell types. Involved in activation of SRC-dependent MAPK signaling on hormone stimulation.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi567 – 639Nuclear receptorPROSITE-ProRule annotationAdd BLAST73
Zinc fingeri567 – 587NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri603 – 627NR C4-typePROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Receptor
Biological processTranscription, Transcription regulation
LigandLipid-binding, Metal-binding, Steroid-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Progesterone receptor
Short name:
PR
Alternative name(s):
Nuclear receptor subfamily 3 group C member 3
Gene namesi
Name:PGR
Synonyms:NR3C3
OrganismiPapio anubis (Olive baboon)
Taxonomic identifieri9555 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaePapio
Proteomesi
  • UP000028761 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003758591 – 933Progesterone receptorAdd BLAST933

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20PhosphoserineBy similarity1
Modified residuei81PhosphoserineBy similarity1
Modified residuei130PhosphoserineBy similarity1
Modified residuei162PhosphoserineBy similarity1
Modified residuei190PhosphoserineBy similarity1
Modified residuei213PhosphoserineBy similarity1
Modified residuei294Phosphoserine; by MAPK1By similarity1
Modified residuei345Phosphoserine; by MAPKBy similarity1
Cross-linki388Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki388Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei400Phosphoserine; by CDK2By similarity1
Cross-linki531Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Post-translational modificationi

Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G2/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1 (By similarity).By similarity
Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294 (By similarity).By similarity
Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294 (By similarity).By similarity
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Interacts with SMARD1 and UNC45A. Interacts with CUEDC2; the interaction promotes ubiquitination, decreases sumoylation, and repesses transcriptional activity. Interacts with PIAS3; the interaction promotes sumoylation of PR in a hormone-dependent manner, inhibits DNA-binding, and alters nuclear export. Interacts with SP1; the interaction requires ligand-induced phosphorylation on Ser-345 by ERK1/2-MAPK. Interacts with PRMT2. Interacts with NCOA2 and NCOA1. Interacts with KLF9.By similarity

Structurei

3D structure databases

ProteinModelPortaliA7X8C7.
SMRiA7X8C7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 566Modulating, Pro-RichAdd BLAST566
Regioni1 – 164AF3; mediates transcriptional activationBy similarityAdd BLAST164
Regioni165 – 305Mediates transcriptional transrepressionBy similarityAdd BLAST141
Regioni456 – 546AF1; mediates transcriptional activationBy similarityAdd BLAST91
Regioni681 – 933Steroid-bindingAdd BLAST253
Regioni687 – 933AF2; mediates transcriptional activationBy similarityAdd BLAST247

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi55 – 59LXXL motif 1By similarity5
Motifi115 – 119LXXL motif 2By similarity5
Motifi183 – 187Nuclear localization signalSequence analysis5

Domaini

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

Sequence similaritiesi

Belongs to the nuclear hormone receptor family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri567 – 587NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri603 – 627NR C4-typePROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiView protein in InterPro
IPR035500. NHR_like_domain.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR000128. Progest_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
PfamiView protein in Pfam
PF00104. Hormone_recep. 1 hit.
PF02161. Prog_receptor. 1 hit.
PF00105. zf-C4. 1 hit.
PRINTSiPR00544. PROGESTRONER.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiView protein in SMART
SM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
SUPFAMiSSF48508. SSF48508. 2 hits.
PROSITEiView protein in PROSITE
PS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A7X8C7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTELKAKGPR APHVAGGPPS PEVGSPLLCR PAAGPFQGSQ TSDTLPEVSA
60 70 80 90 100
IPISLDGLLF PRPCQGQDPL DEKTQDQQSL SDVEGAYSRA EATRGTGGSS
110 120 130 140 150
SRPPEKDSGL LHSVLDTLLA PSGPGQSQPS PPACEVTSSW CLFGPELPED
160 170 180 190 200
PPAAPATQGV LSPLMSRSGC KAGDSSGTAA AHKVLPRGLS PSRQLLLPAS
210 220 230 240 250
GSPHWSGAPV KPSPQPAAVE VEEEDGSESE ESAGPLLKGK PRALGGAAAG
260 270 280 290 300
GGAAAVPPGA AAGGVALVPK EDSRFSAPRV ALVEQDAPMA PGRSPLATTT
310 320 330 340 350
MDFTHVPILP LSHALLAART RQLLEEESYD GGAGAASAFA PPRSSPSASS
360 370 380 390 400
TPVAVGDFPD CAYPPDADPK DDAYPLYGDF QPPALKIKEE EEGAEVSARS
410 420 430 440 450
PRSYLVAGAN PAAFPDFPLG PPPPLPPRAP PSRPGEAAVT AAPAGASVSS
460 470 480 490 500
ASSSGSTLEC ILYKAEGAPP QQGPFAPPPC KAPGAGGCLL PRDGLPSTSA
510 520 530 540 550
SAAAAGAAPA LYPALGLNGL PQLGYQAAVL KEGLQQVYPP YLNYLRPDSE
560 570 580 590 600
ASQSPQYSFE SLPQKICLIC GDEASGCHYG VLTCGSCKVF FKRAMEGQHN
610 620 630 640 650
YLCAGRNDCI VDKIRRKNCP ACRLRKCCQA GMVLGGRKFK KFNKVRVMRA
660 670 680 690 700
LDAVALPQPV GIPNESQALS QRFTFPPGQD IQLIPPLINL LVSIEPDVIY
710 720 730 740 750
AGHDNSKPDT SSSLLTSLNQ LGERQLLSVV KWSKLLPGFR NLHIDDQITL
760 770 780 790 800
IQYSWMSLMV FGLGWRSYKH VSGQMLYFAP DLILNEQRMK ESSFYSLCLT
810 820 830 840 850
MWQIPQEFVK LQVSQEEFLC MKVLLLLNTI PLEGLRSQTQ FEEMRSSYIR
860 870 880 890 900
ELIKAIGLRQ KGVVSSSQRF YQLTKLLDNL HDLVKQLHLY CLNTFIQSRA
910 920 930
LSVEFPEMMS EVIAAQLPKI LAGMVKPLLF HKK
Length:933
Mass (Da):98,950
Last modified:October 23, 2007 - v1
Checksum:i349DB9E6B3ACFEDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ234986 Genomic DNA. Translation: ABB72146.1.
RefSeqiNP_001158062.1. NM_001164590.1.
UniGeneiPan.17938.

Genome annotation databases

GeneIDi100303420.

Similar proteinsi

Entry informationi

Entry nameiPRGR_PAPAN
AccessioniPrimary (citable) accession number: A7X8C7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: October 23, 2007
Last modified: October 25, 2017
This is version 50 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families