Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Large proline-rich protein BAG6

Gene

BAG6

Organism
Ornithorhynchus anatinus (Duckbill platypus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane. Moreover, it regulates the stability and the degradation of proteins by the proteasome. For instance, it is required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. It is also involved in ubiquitin-mediated proteasomal degradation of proteins of the secretory pathway that are mislocalized to the cytosol. Binds the mislocalized proteins, preventing their aggregation in the cytosol, and promotes their ubiquitination. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2.By similarity
Involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2) (By similarity).By similarity
Can be released from tumor and dendritic cells in membrane vesicles or exosomes, and engage NCR3 thereby promoting natural killer cells (NK) activation and cytotoxicity.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Chromatin regulator

Keywords - Biological processi

Apoptosis, Differentiation, Immunity, Spermatogenesis, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Large proline-rich protein BAG6
Alternative name(s):
BCL2-associated athanogene 6
HLA-B-associated transcript 3
Gene namesi
Name:BAG6
Synonyms:BAT3
OrganismiOrnithorhynchus anatinus (Duckbill platypus)
Taxonomic identifieri9258 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaMonotremataOrnithorhynchidaeOrnithorhynchus
Proteomesi
  • UP000002279 Componenti: Unplaced

Subcellular locationi

  • Cytoplasmcytosol By similarity
  • Nucleus By similarity

  • Note: The C-terminal fragment generated by caspase-3 is cytoplasmic. Also found in extracellular vesicular exosomes in some tumor cells (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10881088Large proline-rich protein BAG6PRO_0000403750Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei103 – 1031PhosphoserineBy similarity
Modified residuei354 – 3541PhosphothreonineBy similarity
Modified residuei976 – 9761PhosphoserineBy similarity
Modified residuei1073 – 10731PhosphoserineBy similarity

Post-translational modificationi

Cleavage by caspase-3 releases a C-terminal peptide that plays a role in ricin-induced apoptosis.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei1005 – 10062Cleavage; by caspase-3By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiA7X5R6.

Interactioni

Subunit structurei

Component of the BAT3 complex, at least composed of BAG6/BAT3, UBL4A and GET4/TRC35. Interacts with AIFM1, CTCFL, HSPA2 and p300/EP300. Interacts with ricin A chain. Interacts with NCR3. Interacts (via ubiquitin-like domain) with RNF126; required for BAG6-dependent ubiquitination of proteins mislocalized to the cytosol.By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliA7X5R6.
SMRiA7X5R6. Positions 24-105.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 9976Ubiquitin-likePROSITE-ProRule annotationAdd
BLAST
Repeati243 – 274321Add
BLAST
Repeati415 – 442282Add
BLAST
Repeati571 – 599293Add
BLAST
Repeati607 – 640344Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni243 – 6403984 X 29 AA approximate repeatsAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi197 – 27781Pro-richAdd
BLAST
Compositional biasi398 – 685288Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG002193.
InParanoidiA7X5R6.

Family and domain databases

InterProiIPR021925. DUF3538.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF12057. DUF3538. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7X5R6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPGGGGGGG GPGPGPDMEE PADLEVSVKT LDSQTRTFTV GAEMTVKEFK
60 70 80 90 100
EHIAAAVSIP PDKQRLIYQG RVLQDDKKLQ EYNVGGKVIH LVERAPPQTQ
110 120 130 140 150
GPSSGGASRA GSPSAPHAGA PPAGPRGPGA PVHDRNANSY VMVGTFNLPS
160 170 180 190 200
DGSAVDVHIN MEQAPIQSEP RVRLVMAQHM LRDIQALLAR LEPQPGQQGQ
210 220 230 240 250
QGQQQGQALL AESGPPRPGP PGQTDGETSP REPTETREPT ETREPEDGVA
260 270 280 290 300
ARGTGPAPGP APAPAPEGNA APNHPSPAEY AEVLQELQRV ESRLQPFLQR
310 320 330 340 350
YRAILGAAAT TDYNNNTEGR EEDQRVINLV GESLRLLGNT FVALSDLRCN
360 370 380 390 400
LSATAPRHLH VVRPMSHYTA PMVLQQAAIP IQINVGTTVT MTGSGARPGP
410 420 430 440 450
TDTTPTSGQT SSPTPSPTSG EPGPDGAPSG PAPPQAAGPP RLIRISHQSV
460 470 480 490 500
EPVVMMHMNI PDSGSQTGGT SSASTASTGL PGQGLGQQVS GFPAAPTRVV
510 520 530 540 550
IARPTPPQAR PPHPGGPPPA PGATIPVPGS NASLAQMVSG LVGQLLMQPV
560 570 580 590 600
LVAQGASGLG APQAPATASA SAGTTNTATT AGPAPGGPAQ PPPPPPPGPP
610 620 630 640 650
QAEVQFSQLL GSLLGPGVPG GPGTAGGATS VGSPTITVAM PGVPAFLQGM
660 670 680 690 700
TDFLQATQTA PPPPPPPPPP PAPEQAPAAA PPGSPPAGPG GAGGGPEALP
710 720 730 740 750
PEFFTSVVQG VLSSLLGSLG ARAGSGESIA GFIQRLSGSS NIFEPGADGA
760 770 780 790 800
LGFFGALLSV ICQNLSMVDV VMLLHGHSQP LQRLQPQLRG FFHQHYLGGR
810 820 830 840 850
EPTGPAIRRA THTLITGLEE YVRDSFASVQ VQPGVDITRT NLDFLQEQFN
860 870 880 890 900
GIAAHVLHCT DSSFGVRLLE LCNQGLFECL ALNLHCLGGQ QSALTNVING
910 920 930 940 950
RIRRLSGGVN PSLVSWLTTM MGLRLQVVLE HMPVGPDQVL RYVRRLGEPP
960 970 980 990 1000
QPPPEEPMDV QGAERAPPEP ERENASPAPG TTAEEAMSRG PPPAPEGPPP
1010 1020 1030 1040 1050
LEEQDGAAAA ESEPWAAAVP PEWVPIIRQD LQTQRKVKPQ PPLSDAYLSG
1060 1070 1080
MPAKRRKLRS DLQQRLRADP NYSPQHFPNA QRAFMDEP
Length:1,088
Mass (Da):112,586
Last modified:October 23, 2007 - v1
Checksum:i59144DCD0731671B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU030444 Genomic DNA. Translation: ABU86908.1.
RefSeqiNP_001229675.1. NM_001242746.1.

Genome annotation databases

GeneIDi100529065.
KEGGioaa:100529065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU030444 Genomic DNA. Translation: ABU86908.1.
RefSeqiNP_001229675.1. NM_001242746.1.

3D structure databases

ProteinModelPortaliA7X5R6.
SMRiA7X5R6. Positions 24-105.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiA7X5R6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100529065.
KEGGioaa:100529065.

Organism-specific databases

CTDi7917.

Phylogenomic databases

HOVERGENiHBG002193.
InParanoidiA7X5R6.

Family and domain databases

InterProiIPR021925. DUF3538.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF12057. DUF3538. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Disruption and pseudoautosomal localization of the major histocompatibility complex in monotremes."
    Dohm J.C., Tsend-Ayush E., Reinhardt R., Grutzner F., Himmelbauer H.
    Genome Biol. 8:R175.1-R175.16(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiBAG6_ORNAN
AccessioniPrimary (citable) accession number: A7X5R6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: October 23, 2007
Last modified: November 11, 2015
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.