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Protein

Diacylglycerol kinase

Gene

dagK

Organism
Staphylococcus aureus (strain Mu3 / ATCC 700698)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthetic pathway (By similarity).By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. This ion appears to have a structural role and is required for catalytic activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATPPROSITE-ProRule annotation
Binding sitei94 – 941ATPPROSITE-ProRule annotation
Metal bindingi213 – 2131Magnesium; via carbonyl oxygenBy similarity
Metal bindingi216 – 2161MagnesiumBy similarity
Metal bindingi218 – 2181Magnesium; via carbonyl oxygenBy similarity
Active sitei273 – 2731Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 145ATPPROSITE-ProRule annotation
Nucleotide bindingi67 – 737ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR418127:GJP9-1939-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase (EC:2.7.1.107)
Short name:
DAG kinase
Short name:
DAGK
Gene namesi
Name:dagK
Ordered Locus Names:SAHV_1883
OrganismiStaphylococcus aureus (strain Mu3 / ATCC 700698)
Taxonomic identifieri418127 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Diacylglycerol kinasePRO_0000386485Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliA7X424.
SMRiA7X424. Positions 1-312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 132132DAGKcPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the diacylglycerol/lipid kinase family.Curated
Contains 1 DAGKc domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1597.
HOGENOMiHOG000261395.
KOiK07029.
OMAiPGEFVNL.
OrthoDBiEOG613098.

Family and domain databases

Gene3Di3.40.50.10330. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR00147. TIGR00147. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7X424-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKRARIIYN PTSGKELFKR ELPDALIKLE KAGYETSAYA TEKIGDATLE
60 70 80 90 100
AERAMHENYD VLIAAGGDGT LNEVVNGIAE KPNRPKLGVI PMGTVNDFGR
110 120 130 140 150
ALHIPNDIMG ALDVIIEGHS TKVDIGKMNN RYFINLAAGG QLTQVSYETP
160 170 180 190 200
SKLKSIVGPF AYYIKGFEML PQMKAVDLRI EYDGNVFQGE ALLFFLGLTN
210 220 230 240 250
SMAGFEKLVP DAKLDDGYFT LIIVEKSNLA ELGHIMTLAS RGEHTKHPKV
260 270 280 290 300
IYEKAKAINI SSFTDLQLNV DGEYGGKLPA NFLNLERHID VFAPNDIVNE
310
ELINNDHVDD NLIEE
Length:315
Mass (Da):34,887
Last modified:October 23, 2007 - v1
Checksum:iC5CC706B5E3A0F00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009324 Genomic DNA. Translation: BAF78766.1.
RefSeqiWP_001231451.1. NC_009782.1.

Genome annotation databases

EnsemblBacteriaiBAF78766; BAF78766; SAHV_1883.
GeneIDi23197755.
KEGGisaw:SAHV_1883.
PATRICi19558914. VBIStaAur127830_1937.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009324 Genomic DNA. Translation: BAF78766.1.
RefSeqiWP_001231451.1. NC_009782.1.

3D structure databases

ProteinModelPortaliA7X424.
SMRiA7X424. Positions 1-312.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF78766; BAF78766; SAHV_1883.
GeneIDi23197755.
KEGGisaw:SAHV_1883.
PATRICi19558914. VBIStaAur127830_1937.

Phylogenomic databases

eggNOGiCOG1597.
HOGENOMiHOG000261395.
KOiK07029.
OMAiPGEFVNL.
OrthoDBiEOG613098.

Enzyme and pathway databases

BioCyciSAUR418127:GJP9-1939-MONOMER.

Family and domain databases

Gene3Di3.40.50.10330. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR00147. TIGR00147. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Mutated response regulator graR is responsible for phenotypic conversion of Staphylococcus aureus from heterogeneous vancomycin-intermediate resistance to vancomycin-intermediate resistance."
    Neoh H.-M., Cui L., Yuzawa H., Takeuchi F., Matsuo M., Hiramatsu K.
    Antimicrob. Agents Chemother. 52:45-53(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Mu3 / ATCC 700698.

Entry informationi

Entry nameiDAGK_STAA1
AccessioniPrimary (citable) accession number: A7X424
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: October 23, 2007
Last modified: July 22, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.