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A7X3I5 (TRMB_STAA1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:SAHV_1734
OrganismStaphylococcus aureus (strain Mu3 / ATCC 700698) [Complete proteome] [HAMAP]
Taxonomic identifier418127 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Staphylococcus ›

Protein attributes

Sequence length214 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 214214tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000136361

Regions

Region190 – 1934Substrate binding Potential

Sites

Binding site431S-adenosyl-L-methionine By similarity
Binding site681S-adenosyl-L-methionine By similarity
Binding site951S-adenosyl-L-methionine By similarity
Binding site1171S-adenosyl-L-methionine By similarity
Binding site1211Substrate By similarity
Binding site1531Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
A7X3I5 [UniParc].

Last modified October 23, 2007. Version 1.
Checksum: 6E0EA5A49A3FF264

FASTA21425,291
        10         20         30         40         50         60 
MRVRYKPWAE DYLKDHPELV DMEGQHAGEM TEWFDKTQPI HIEIGSGMGQ FITTLAAQNP 

        70         80         90        100        110        120 
HINYISMERE KSIVYKVLDK VKEMGLTNLK IICNDAIELN EYFKDGEVSR IYLNFSDPWP 

       130        140        150        160        170        180 
KNRHAKRRLT YHTFLALYQQ ILNDEGDLHF KTDNRGLFAY SLESMSQFGM YFTKINLNLH 

       190        200        210 
QEDDGSNILT EYEKKFSDKG SRIYRMEAKF HSQK

« Hide

References

[1]"Mutated response regulator graR is responsible for phenotypic conversion of Staphylococcus aureus from heterogeneous vancomycin-intermediate resistance to vancomycin-intermediate resistance."
Neoh H.-M., Cui L., Yuzawa H., Takeuchi F., Matsuo M., Hiramatsu K.
Antimicrob. Agents Chemother. 52:45-53(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Mu3 / ATCC 700698.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP009324 Genomic DNA. Translation: BAF78617.1.
RefSeqYP_001442324.1. NC_009782.1.

3D structure databases

ProteinModelPortalA7X3I5.
SMRA7X3I5. Positions 9-210.
ModBaseSearch...

Protein-protein interaction databases

STRING418127.SAHV_1734.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF78617; BAF78617; SAHV_1734.
GeneID5560682.
KEGGsaw:SAHV_1734.
PATRIC19558526. VBIStaAur127830_1781.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMARLRNKPW.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycSAUR418127:GJP9-1750-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMB_STAA1
AccessionPrimary (citable) accession number: A7X3I5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 23, 2007
Last modified: May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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