A7X306 (MTNN_STAA1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Short name=MTA/SAH nucleosidase
Short name=MTAN
EC=3.2.2.9

Alternative name(s):
5'-methylthioadenosine nucleosidase
Short name=MTA nucleosidase
S-adenosylhomocysteine nucleosidase
Short name=AdoHcy nucleosidase
Short name=SAH nucleosidase
Short name=SRH nucleosidase

Gene names

Name:	mtnN
Ordered Locus Names:	SAHV_1586

Organism

Staphylococcus aureus (strain Mu3 / ATCC 700698) [Complete proteome] [HAMAP]

Taxonomic identifier

418127 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively By similarity. HAMAP MF_01684
Catalytic activity	S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine. HAMAP MF_01684 S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine. HAMAP MF_01684
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2. HAMAP MF_01684
Sequence similarities	Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-methionine salvage from methylthioadenosine Inferred from electronic annotation. Source: InterPro nucleoside catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenosylhomocysteine nucleosidase activity Inferred from electronic annotation. Source: EC methylthioadenosine nucleosidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase HAMAP MF_01684		PRO_0000359368
Regions
	Region	172 – 173	2	Substrate binding By similarity
Sites
	Active site	11	1	Proton acceptor By similarity
	Binding site	77	1	Substrate; via amide nitrogen By similarity
	Binding site	151	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	196	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7X306 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: C756E9386E9B19DD	FASTA	228	24,534
	10 20 30 40 50 60 MIGIIGAMEE EVTILKNKLT QLSEISVAHV KFYTGILKDR EVVITQSGIG KVNAAISTTL 70 80 90 100 110 120 LINKFKPDVI INTGSAGALD ESLNVGDVLI SDDVKYHDAD ATAFGYEYGQ IPQMPVAFQS 130 140 150 160 170 180 SKPLIEKVSQ VVQQQQLTAK VGLIVSGDSF IGSVEQRQKI KKAFPNAMAV EMEATAIAQT 190 200 210 220 CYQFNVPFVV VRAVSDLANG EAEMSFEAFL EKAAVSSSQT VEALVSQL

References

[1]

"Mutated response regulator graR is responsible for phenotypic conversion of Staphylococcus aureus from heterogeneous vancomycin-intermediate resistance to vancomycin-intermediate resistance." Neoh H.-M., Cui L., Yuzawa H., Takeuchi F., Matsuo M., Hiramatsu K. Antimicrob. Agents Chemother. 52:45-53(2008) [PubMed: 17954695] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Mu3 / ATCC 700698.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009324 Genomic DNA. Translation: BAF78469.1.
RefSeq	YP_001442176.1. NC_009782.1.
3D structure databases
ProteinModelPortal	A7X306.
SMR	A7X306. Positions 2-227.
ModBase	Search...
Protein-protein interaction databases
STRING	A7X306.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000004639; EBSTAP00000004538; EBSTAG00000004639.
GeneID	5559801.
GenomeReviews	Gene locus SAHV_1586 in contig AP009324_GR.
KEGG	saw:SAHV_1586.
PATRIC	19558214. VBIStaAur127830_1625.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0775.
GeneTree	EBGT00050000025335.
HOGENOM	HBG367723.
OMA	LEHFPTM.
ProtClustDB	CLSK885455.
Enzyme and pathway databases
BioCyc	SAUR418127:SAHV_1586-MONOMER.
Family and domain databases
HAMAP	MF_01684. Salvage_tnN. [Tree]
InterPro	IPR010049. MTA_SAH_Nsdase. IPR018017. Nucleoside_phosphorylase. IPR000845. Nucleoside_phosphorylase_d. [Graphical view]
KO	K01243.
PANTHER	PTHR21234. PNP_UDP. 1 hit. PTHR21234:SF6. PTHR21234:SF6. 1 hit.
Pfam	PF01048. PNP_UDP_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01704. MTA/SAH-Nsdase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTNN_STAA1

Accession

Primary (citable) accession number: A7X306

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program