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Protein

Probable endonuclease 4

Gene

nfo

Organism
Staphylococcus aureus (strain Mu3 / ATCC 700698)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 3 Zn2+ ions.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi68 – 681Zinc 1UniRule annotation
Metal bindingi109 – 1091Zinc 1UniRule annotation
Metal bindingi144 – 1441Zinc 1UniRule annotation
Metal bindingi144 – 1441Zinc 2UniRule annotation
Metal bindingi178 – 1781Zinc 2UniRule annotation
Metal bindingi181 – 1811Zinc 3UniRule annotation
Metal bindingi213 – 2131Zinc 2UniRule annotation
Metal bindingi226 – 2261Zinc 3UniRule annotation
Metal bindingi228 – 2281Zinc 3UniRule annotation
Metal bindingi258 – 2581Zinc 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciSAUR418127:GJP9-1561-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endonuclease 4UniRule annotation (EC:3.1.21.2UniRule annotation)
Alternative name(s):
Endodeoxyribonuclease IVUniRule annotation
Endonuclease IVUniRule annotation
Gene namesi
Name:nfoUniRule annotation
Ordered Locus Names:SAHV_1544
OrganismiStaphylococcus aureus (strain Mu3 / ATCC 700698)
Taxonomic identifieri418127 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296Probable endonuclease 4PRO_1000011336Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA7X2V2.
SMRiA7X2V2. Positions 3-293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AP endonuclease 2 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000224895.
KOiK01151.
OMAiMKYVGAH.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo. 1 hit.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7X2V2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLGSHVSMS GKKMLEGSAI EAYEYGETTF MIYTGAPQNT RRKSIEDLNI
60 70 80 90 100
TKGHEVMEKY GLSNIVVHAP YIINIANTTK PETFNLGVDF LQQEIERTQA
110 120 130 140 150
IGAKDIVLHP GAHVGAGVDA GINKIIEGLN EVLTNDNNVR IALETMAGKG
160 170 180 190 200
TEIGRSFEEL ARIIDGVHNN ERLSVCFDTC HTHDAGYNVK EDFDGVLNEF
210 220 230 240 250
DKIIGVDRIK VVHVNDSKND RGAQKDRHEN IGFGYIGFDA LNYIVHHDSF
260 270 280 290
KDIPKILETP YVGEDKKNKK PPYKLEIEML KQQQFDPELK NKVMQQ
Length:296
Mass (Da):33,185
Last modified:October 23, 2007 - v1
Checksum:i6B32D9961F9BEC3C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009324 Genomic DNA. Translation: BAF78427.1.
RefSeqiWP_000924220.1. NC_009782.1.

Genome annotation databases

EnsemblBacteriaiBAF78427; BAF78427; SAHV_1544.
KEGGisaw:SAHV_1544.
PATRICi19558128. VBIStaAur127830_1581.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009324 Genomic DNA. Translation: BAF78427.1.
RefSeqiWP_000924220.1. NC_009782.1.

3D structure databases

ProteinModelPortaliA7X2V2.
SMRiA7X2V2. Positions 3-293.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF78427; BAF78427; SAHV_1544.
KEGGisaw:SAHV_1544.
PATRICi19558128. VBIStaAur127830_1581.

Phylogenomic databases

HOGENOMiHOG000224895.
KOiK01151.
OMAiMKYVGAH.

Enzyme and pathway databases

BioCyciSAUR418127:GJP9-1561-MONOMER.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo. 1 hit.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEND4_STAA1
AccessioniPrimary (citable) accession number: A7X2V2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 23, 2007
Last modified: September 7, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.