A7X2B3 (TYSY_STAA1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	SAHV_1415

Organism

Staphylococcus aureus (strain Mu3 / ATCC 700698) [Complete proteome] [HAMAP]

Taxonomic identifier

418127 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Thymidylate synthase HAMAP-Rule MF_00008		PRO_1000000685
Sites
	Active site	201	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7X2B3 [UniParc]. Last modified October 23, 2007. Version 1. Checksum: E7F41BA915C25D37	FASTA	318	36,825
	10 20 30 40 50 60 MLNSFDAAYH SLCEEVLEIG NTRNDRTNTG TISKFGHQLR FDLSKGFPLL TTKKVSFKLV 70 80 90 100 110 120 ATELLWFIKG DTNIQYLLKY NNNIWNEWAF ENYIKSDEYN GPDMTDFGHR ALSDPEFNEQ 130 140 150 160 170 180 YKEQMKQFKQ RILEDDTFAK QFGDLGNVYG KQWRDWVDKD GNHFDQLKTV IEQIKHNPDS 190 200 210 220 230 240 RRHIVSAWNP TEIDTMALPP CHTMFQFYVQ DGKLSCQLYQ RSADIFLGVP FNIASYALLT 250 260 270 280 290 300 HLIAKECGLE VGEFVHTFGD AHIYSNHIDA IQTQLARESF NPPTLKINSD KSIFDINYED 310 LEIVDYESHP AIKAPIAV

References

[1]

"Mutated response regulator graR is responsible for phenotypic conversion of Staphylococcus aureus from heterogeneous vancomycin-intermediate resistance to vancomycin-intermediate resistance." Neoh H.-M., Cui L., Yuzawa H., Takeuchi F., Matsuo M., Hiramatsu K. Antimicrob. Agents Chemother. 52:45-53(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Mu3 / ATCC 700698.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009324 Genomic DNA. Translation: BAF78298.1.
RefSeq	YP_001442005.1. NC_009782.1.
3D structure databases
ProteinModelPortal	A7X2B3.
SMR	A7X2B3. Positions 3-314.
ModBase	Search...
Protein-protein interaction databases
STRING	418127.SAHV_1415.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAF78298; BAF78298; SAHV_1415.
GeneID	5561632.
KEGG	saw:SAHV_1415.
PATRIC	19557862. VBIStaAur127830_1448.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HOG000257899.
KO	K00560.
OMA	DTNVAYL.
ProtClustDB	PRK01827.
Enzyme and pathway databases
BioCyc	SAUR418127:GJP9-1433-MONOMER.
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 2 hits.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_STAA1

Accession

Primary (citable) accession number: A7X2B3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 23, 2007
Last modified:	May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program