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A7X271 (DAPA_STAA1) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52
Gene names
Name:dapA
Ordered Locus Names:SAHV_1383
OrganismStaphylococcus aureus (strain Mu3 / ATCC 700698) [Complete proteome] [HAMAP]
Taxonomic identifier418127 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length295 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418

Subunit structure

Homotetramer By similarity. HAMAP MF_00418

Subcellular location

Cytoplasm By similarity HAMAP MF_00418.

Sequence similarities

Belongs to the DHDPS family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdiaminopimelate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydrodipicolinate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 295295Dihydrodipicolinate synthase HAMAP MF_00418
PRO_1000050277

Regions

Region50 – 512Pyruvate binding By similarity

Sites

Active site1631Schiff-base intermediate with substrate By similarity
Binding site1081Pyruvate By similarity
Site1351Involved in proton transfer during cleavage By similarity

Sequences

Sequence LengthMass (Da)Tools
A7X271 [UniParc].

Last modified October 23, 2007. Version 1.
Checksum: EAD1E6DD4CE05A17

FASTA29532,660
        10         20         30         40         50         60 
MTHLFEGVGV ALTTPFTNNK VNLEALKAHV NFLLENNAQA IIVNGTTAES PTLTTDEKER 

        70         80         90        100        110        120 
ILKTVIDLVD KRVPVIAGTG TNDTEKSIQA SFQAKALGAD AIMLITPYYN KTNQRGLVKH 

       130        140        150        160        170        180 
FEAITDAVKL PVVLYNVPSR TNMTIEPETV EILSQHPYIV ALKDATNDFE YLEEVKKRID 

       190        200        210        220        230        240 
TNSFALYSGN DDNVVEYYQR GGQGVISVIA NVIPKEFQAL YDAQQSGLDI QDQFKPIGTL 

       250        260        270        280        290 
LSALSVDINP IPIKALTSYL EFGNYELRLP LVSLEDTDTK VLREAYDTFK AGENE

« Hide

References

[1]"Mutated response regulator graR is responsible for phenotypic conversion of Staphylococcus aureus from heterogeneous vancomycin-intermediate resistance to vancomycin-intermediate resistance."
Neoh H.-M., Cui L., Yuzawa H., Takeuchi F., Matsuo M., Hiramatsu K.
Antimicrob. Agents Chemother. 52:45-53(2008) [PubMed: 17954695] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Mu3 / ATCC 700698.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP009324 Genomic DNA. Translation: BAF78266.1.
RefSeqYP_001441973.1. NC_009782.1.

3D structure databases

ProteinModelPortalA7X271.
SMRA7X271. Positions 2-293.
ModBaseSearch...

Protein-protein interaction databases

STRINGA7X271.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBSTAT00000004871; EBSTAP00000004770; EBSTAG00000004870.
GeneID5561606.
GenomeReviewsGene locus SAHV_1383 in contig AP009324_GR.
KEGGsaw:SAHV_1383.
PATRIC19557794. VBIStaAur127830_1414.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0329.
GeneTreeEBGT00050000024245.
HOGENOMHBG358848.
OMATIHFTHI.
ProtClustDBPRK03170.

Enzyme and pathway databases

BioCycSAUR418127:SAHV_1383-MONOMER.

Family and domain databases

HAMAPMF_00418. DapA.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01714.
PANTHERPTHR12128. DHDPS. 1 hit.
PfamPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFPIRSF001365. DHDPS. 1 hit.
PRINTSPR00146. DHPICSNTHASE.
TIGRFAMsTIGR00674. DapA. 1 hit.
PROSITEPS00665. DHDPS_1. False negative.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPA_STAA1
AccessionPrimary (citable) accession number: A7X271
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 23, 2007
Last modified: January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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