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Protein

Beta-hexosaminidase 1

Gene

HEXO1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a broad substrate specificity. Can use synthetic substrates such as pyridylaminated chitotriose, pyridylaminated chitobiose, p-nitrophenyl-beta-N-acetylglucosaminide, p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside (pNP-GlcNAc), p-nitrophenyl-2-acetamido-2-deoxy-beta-D-galactopyranoside (pNP-GalNAc), 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside (MU-GlcNAc), and 4-methylumbelliferyl-6-sulfo-2-acetamido-2-deoxy-beta-D-glucopyranoside (MU-GlcNAc-6SO4) as substrates. Removes terminal GlcNAc residues from alpha1,3- and alpha1,6-mannosyl branches of biantennary N-glycans without any strict branch preference. Required for the presence of paucimannosidic N-glycans in glycoproteins of roots and, to a lower extent, of leaves.3 Publications

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.1 Publication

Enzyme regulationi

Inhibited by N-acetylcastanospermine, 2-acet-amido-1,2-dideoxynojirimycin and PUGNAc.1 Publication

Kineticsi

  1. KM=0.7 mM for pNP-GlcNAc (at pH 4.6 and 37 degrees Celsius)2 Publications
  1. Vmax=151 µmol/min/mg enzyme with pNP-GlcNAc as substrate (at pH 4.6 and 37 degrees Celsius)2 Publications

pH dependencei

Optimum pH is 4-5.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei332Proton donorBy similarity1

GO - Molecular functioni

  • beta-N-acetylhexosaminidase activity Source: TAIR
  • hexosaminidase activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT3G55260-MONOMER.
BRENDAi3.2.1.52. 399.
ReactomeiR-ATH-1660662. Glycosphingolipid metabolism.
R-ATH-2022857. Keratan sulfate degradation.
R-ATH-2024101. CS/DS degradation.
R-ATH-2160916. Hyaluronan uptake and degradation.
R-ATH-6798695. Neutrophil degranulation.

Protein family/group databases

CAZyiGH20. Glycoside Hydrolase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-hexosaminidase 1 (EC:3.2.1.52)
Alternative name(s):
Beta-GlcNAcase 1
Beta-N-acetylhexosaminidase 1
Beta-hexosaminidase 2
Short name:
AtHEX2
N-acetyl-beta-glucosaminidase 1
Gene namesi
Name:HEXO1
Synonyms:HEX2
Ordered Locus Names:At3g55260
ORF Names:T26I12.140
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G55260.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • plant-type cell wall Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

Pathology & Biotechi

Disruption phenotypei

Reduced amounts of paucimannosidic N-glycans-containing glycoproteins in roots and, to a lower extent, in leaves.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000042028621 – 541Beta-hexosaminidase 1Add BLAST521

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi44N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi295 ↔ 337By similarity
Glycosylationi304N-linked (GlcNAc...)Sequence analysis1
Glycosylationi340N-linked (GlcNAc...)Sequence analysis1
Glycosylationi352N-linked (GlcNAc...)Sequence analysis1
Glycosylationi497N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi511 ↔ 538By similarity

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiA7WM73.
PRIDEiA7WM73.
ProMEXiA7WM73.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems, flowers and siliques.1 Publication

Gene expression databases

GenevisibleiA7WM73. AT.

Interactioni

Protein-protein interaction databases

IntActiA7WM73. 1 interactor.
STRINGi3702.AT3G55260.1.

Structurei

3D structure databases

ProteinModelPortaliA7WM73.
SMRiA7WM73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 20 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2499. Eukaryota.
COG3525. LUCA.
HOGENOMiHOG000157972.
InParanoidiA7WM73.
KOiK12373.
OMAiWTPVNWE.
OrthoDBiEOG093609RX.
PhylomeDBiA7WM73.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProiIPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR029019. HEX_eukaryotic_N.
[Graphical view]
PfamiPF00728. Glyco_hydro_20. 1 hit.
PF14845. Glycohydro_20b2. 1 hit.
[Graphical view]
PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSiPR00738. GLHYDRLASE20.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A7WM73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTNLLRLIL LFITLSITSS LSTPSPADSP PYLWPLPAEF SFGNETLSVD
60 70 80 90 100
PTVTLIVAGN GGGSLIIRAA FDRYMGIIFK HASGRGSLLS RIRFLKMVEY
110 120 130 140 150
DITSLKIVVH SDSEELQLGV DESYTLMVSK KNEQSIVGAA TIEANTVYGA
160 170 180 190 200
LRGLETFSQL CAFDYITKSV QIYKAPWYIQ DKPRFGYRGL LIDTSRHYLP
210 220 230 240 250
IDVIKQIIES MSFAKLNVLH WHIVDEQSFP LETPTYPNLW KGAYSRWERY
260 270 280 290 300
TVEDASEIVR FAKMRGINVM AEVDVPGHAE SWGTGYPDLW PSLSCREPLD
310 320 330 340 350
VTKNFTFDVI SGILADMRKI FPFELFHLGG DEVNTDCWKN TTHVKEWLQG
360 370 380 390 400
RNFTTKDAYK YFVLRAQQIA ISKNWTPVNW EETFSSFGKD LDPRTVIQNW
410 420 430 440 450
LVSDICQKAV AKGFRCIFSN QGYWYLDHLD VPWEEVYNTE PLNGIEDPSL
460 470 480 490 500
QKLVIGGEVC MWGETADTSV VLQTIWPRAA AAAERMWSTR EAVSKGNITL
510 520 530 540
TALPRLHYFR CLLNNRGVPA APVDNFYARR PPLGPGSCYA Q
Length:541
Mass (Da):61,230
Last modified:October 23, 2007 - v1
Checksum:i441D48C6F1FCCF56
GO

Sequence cautioni

The sequence AAM61367 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAB75760 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84G → V in AAM61367 (Ref. 5) Curated1
Sequence conflicti181D → G in BAE99290 (Ref. 4) Curated1
Sequence conflicti347W → R in BAE99290 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM493720 mRNA. Translation: CAM35467.1.
AL132954 Genomic DNA. Translation: CAB75760.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79360.1.
AK227260 mRNA. Translation: BAE99290.1.
AY084801 mRNA. Translation: AAM61367.1. Different initiation.
BT000920 mRNA. Translation: AAN41320.1.
PIRiT47665.
RefSeqiNP_567017.2. NM_115384.4.
UniGeneiAt.21628.

Genome annotation databases

EnsemblPlantsiAT3G55260.1; AT3G55260.1; AT3G55260.
GeneIDi824692.
GrameneiAT3G55260.1; AT3G55260.1; AT3G55260.
KEGGiath:AT3G55260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM493720 mRNA. Translation: CAM35467.1.
AL132954 Genomic DNA. Translation: CAB75760.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79360.1.
AK227260 mRNA. Translation: BAE99290.1.
AY084801 mRNA. Translation: AAM61367.1. Different initiation.
BT000920 mRNA. Translation: AAN41320.1.
PIRiT47665.
RefSeqiNP_567017.2. NM_115384.4.
UniGeneiAt.21628.

3D structure databases

ProteinModelPortaliA7WM73.
SMRiA7WM73.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiA7WM73. 1 interactor.
STRINGi3702.AT3G55260.1.

Protein family/group databases

CAZyiGH20. Glycoside Hydrolase Family 20.

Proteomic databases

PaxDbiA7WM73.
PRIDEiA7WM73.
ProMEXiA7WM73.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G55260.1; AT3G55260.1; AT3G55260.
GeneIDi824692.
GrameneiAT3G55260.1; AT3G55260.1; AT3G55260.
KEGGiath:AT3G55260.

Organism-specific databases

TAIRiAT3G55260.

Phylogenomic databases

eggNOGiKOG2499. Eukaryota.
COG3525. LUCA.
HOGENOMiHOG000157972.
InParanoidiA7WM73.
KOiK12373.
OMAiWTPVNWE.
OrthoDBiEOG093609RX.
PhylomeDBiA7WM73.

Enzyme and pathway databases

BioCyciARA:AT3G55260-MONOMER.
BRENDAi3.2.1.52. 399.
ReactomeiR-ATH-1660662. Glycosphingolipid metabolism.
R-ATH-2022857. Keratan sulfate degradation.
R-ATH-2024101. CS/DS degradation.
R-ATH-2160916. Hyaluronan uptake and degradation.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiA7WM73.

Gene expression databases

GenevisibleiA7WM73. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProiIPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR029019. HEX_eukaryotic_N.
[Graphical view]
PfamiPF00728. Glyco_hydro_20. 1 hit.
PF14845. Glycohydro_20b2. 1 hit.
[Graphical view]
PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSiPR00738. GLHYDRLASE20.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHEXO1_ARATH
AccessioniPrimary (citable) accession number: A7WM73
Secondary accession number(s): Q0WUA8, Q8LFK0, Q9M3C5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: October 23, 2007
Last modified: November 30, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.