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A7VVD0 (A7VVD0_9CLOT) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase HAMAP-Rule MF_01228
EC=6.1.1.10 HAMAP-Rule MF_01228
Alternative name(s):
Methionyl-tRNA synthetase HAMAP-Rule MF_01228
Gene names
Name:metG HAMAP-Rule MF_01228 EMBL EDO60931.1
ORF Names:CLOLEP_02543 EMBL EDO60931.1
OrganismClostridium leptum DSM 753 EMBL EDO60931.1
Taxonomic identifier428125 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium ›

Protein attributes

Sequence length650 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_01228 SAAS SAAS002547

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_01228 SAAS SAAS002547

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_01228

Subunit structure

Homodimer By similarity. SAAS SAAS002547

Monomer By similarity. HAMAP-Rule MF_01228

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01228.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily. HAMAP-Rule MF_01228

Contains 1 tRNA-binding domain. HAMAP-Rule MF_01228 SAAS SAAS002547

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data. EMBL EDO60931.1

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain550 – 650101tRNA-binding By similarity HAMAP-Rule MF_01228
Motif13 – 2311"HIGH" region By similarity HAMAP-Rule MF_01228
Motif298 – 3025"KMSKS" region By similarity HAMAP-Rule MF_01228

Sites

Metal binding1281Zinc By similarity HAMAP-Rule MF_01228
Metal binding1311Zinc By similarity HAMAP-Rule MF_01228
Metal binding1451Zinc By similarity HAMAP-Rule MF_01228
Metal binding1481Zinc By similarity HAMAP-Rule MF_01228

Sequences

Sequence LengthMass (Da)Tools
A7VVD0 [UniParc].

Last modified October 23, 2007. Version 1.
Checksum: 0B91E6F6B5E77640

FASTA65072,983
        10         20         30         40         50         60 
MDQKTFYITT PIYYPSGKAH IGHSYCTVAT DAIARYKRMQ GYDVMFLTGT DEHGQKIELN 

        70         80         90        100        110        120 
AAKAGVTPKE YVDEIVAGFQ ELWKLLNISN DRFIRTTDDY HVKAVQKIFK ELYDRGEIYK 

       130        140        150        160        170        180 
GKYEGWYCTP CESFWTETQL KDGKCPDCGR EVKWAEEEAY FFKLGNYADR LLKLYEDQPD 

       190        200        210        220        230        240 
FIQPESRKNE MIQFIKNGLD DLCVSRTSFT WGIPVDFDPK HVVYVWLDAL TNYITAMGYG 

       250        260        270        280        290        300 
SGDDSDYRKY WPADVHFIGK EIVRFHTIIW PAILMALDLP LPKQVYGHGW LLFGDGSKMS 

       310        320        330        340        350        360 
KSKGNVVDPV ILCDRYGVDA IRYFLLREIP FGADGLFTNE ALINRINYDL ANDLGNLLSR 

       370        380        390        400        410        420 
TTAMVQKYFG GSIPAQRQAE DLDQDLVAMA TSLRGRYEES MDGFQFSNAL AEVWKLIARS 

       430        440        450        460        470        480 
NKYIDETMPW ALGKAPDKAP RLAAVLYNLC ECLRIVSILI TPFMPETAPK IQAQIGAGAD 

       490        500        510        520        530        540 
VCNWDSADQW GLLPADAVVS KGETLFPRID VAKEIAELDE ILIQKPAEKK AEAPKREQPV 

       550        560        570        580        590        600 
EGVAMIGIDD FAKVELRVAE IIACEPVKRA KKLLKLTLND GQGQRVVASG IAKYYTPDQL 

       610        620        630        640        650 
VGRHVVLVAN LKPAVLCGVE SCGMILAADC AGGDVKVIFV DEIPAGSKIR

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References

[1]"Draft genome sequence of Clostridium leptum (DSM 753)."
Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D., Gordon J.
Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: DSM 753 EMBL EDO60931.1.
[2]Fulton L., Clifton S., Fulton B., Xu J., Minx P., Pepin K.H., Johnson M., Thiruvilangam P., Bhonagiri V., Nash W.E., Wang C., Mardis E.R., Wilson R.K.
Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: DSM 753 EMBL EDO60931.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		ABCB02000019 Genomic DNA. Translation: EDO60931.1.

3D structure databases

ProteinModelPortalA7VVD0.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEDO60931; EDO60931; CLOLEP_02543.
PATRIC30585304. VBICloLep22705_1967.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_01228. Met_tRNA_synth_type2.
InterProIPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEPS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA7VVD0_9CLOT
AccessionPrimary (citable) accession number: A7VVD0
Entry history
Integrated into UniProtKB/TrEMBL: October 23, 2007
Last sequence update: October 23, 2007
Last modified: April 3, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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