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A7UD81 (A7UD81_PLAFA) Unreviewed, UniProtKB/TrEMBL

Last modified March 19, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional dihydrofolate reductase-thymidylate synthase PIRNR PIRNR000389
Gene names
Name:DHFR-TS EMBL ABU43153.1
Synonyms:dhfr-ts EMBL ACG59401.1
OrganismPlasmodium falciparum EMBL ABU43153.1
Taxonomic identifier5833 [NCBI]
Taxonomic lineageEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiumPlasmodium (Laverania)

Protein attributes

Sequence length608 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism By similarity. PIRNR PIRNR000389

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1. PIRNR PIRNR000389

Sequence similarities

In the C-terminal section; belongs to the thymidylate synthase family. PIRNR PIRNR000389

In the N-terminal section; belongs to the dihydrofolate reductase family. PIRNR PIRNR000389

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding44 – 452NADP PDB 4DPD PDB 3UM8 PDB 3UM6 PDB 3QGT PDB 3UM5
Nucleotide binding106 – 1083NADP PDB 4DPD PDB 3UM8 PDB 3UM6 PDB 3QGT PDB 3UM5
Nucleotide binding127 – 1304NADP PDB 4DPD PDB 3UM8 PDB 3UM6 PDB 3QGT PDB 3UM5
Nucleotide binding166 – 1727NADP PDB 4DPD PDB 3UM8 PDB 3UM6 PDB 3QGT PDB 3UM5
Region470 – 4712Phosphate 1 binding PDB 3UM8
Region510 – 5112Phosphate 2 binding PDB 3UM8

Sites

Active site4901 By similarity PIRSR PIRSR000389-1
Binding site161NADP; via amide nitrogen and carbonyl oxygen PDB 4DPD PDB 3UM8 PDB 3QGT
Binding site401NADP; via carbonyl oxygen PDB 4DPD PDB 3UM8 PDB 3UM6 PDB 3QGT PDB 3UM5
Binding site1441NADP; via carbonyl oxygen PDB 4DPD PDB 3QGT PDB 3UM5

Sequences

Sequence LengthMass (Da)Tools
A7UD81 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: 26FD4BB4A5F1F4A7

FASTA60871,737
        10         20         30         40         50         60 
MMEQVCDVFD IYAICACCKV ESKNEGKKNE VFNNYTFRGL GNKGVLPWKC NSLDMKYFCA 

        70         80         90        100        110        120 
VTTYVNESKY EKLKYKRCKY LNKETVDNVN DMPNSKKLQN VVVMGRTSWE SIPKKFKPLS 

       130        140        150        160        170        180 
NRINVILSRT LKKEDFDEDV YIINKVEDLI VLLGKLNYYK CFIIGGSVVY QEFLEKKLIK 

       190        200        210        220        230        240 
KIYFTRINST YECDVFFPEI NENEYQIISV SDVYTSNNTT LDFIIYKKTN NKMLNEQNCI 

       250        260        270        280        290        300 
KGEEKNNDMP LKNDDKDTCH MKKLTEFYKN VDKYKINYEN DDDDEEEDDF VYFNFNKEKE 

       310        320        330        340        350        360 
EKNKNSIHPN DFQIYNSLKY KYHPEYQYLN IIYDIMMNGN KQSDRTGVGV LSKFGYIMKF 

       370        380        390        400        410        420 
DLSQYFPLLT TKKLFLRGII EELLWFIRGE TNGNTLLNKN VRIWEANGTR EFLDNRKLFH 

       430        440        450        460        470        480 
REVNDLGPIY GFQWRHFGAE YTNMYDNYEN KGVDQLKNII NLIKNDPTSR RILLCAWNVK 

       490        500        510        520        530        540 
DLDQMALPPC HILCQFYVFD GKLSCIMYQR SCDLGLGVPF NIASYSIFTH MIAQVCNLQP 

       550        560        570        580        590        600 
AQFIHVLGNA HVYNNHIDSL KIQLNRIPYP FPTLKLNPDI KNIEDFTISD FTIQNYVHHE 


KISMDMAA 

« Hide

References

[1]"Rapid dissemination of Plasmodium falciparum drug resistance despite strictly controlled antimalarial use."
Noranate N., Durand R., Tall A., Marrama L., Spiegel A., Sokhna C., Pradines B., Cojean S., Guillotte M., Bischoff E., Ekala M.T., Bouchier C., Fandeur T., Ariey F., Patarapotikul J., Le Bras J., Trape J.F., Rogier C., Mercereau-Puijalon O.
PLoS ONE 2:e139-e139(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 14290 EMBL ABU43153.1, 18308 EMBL ABU43155.1, 44200 EMBL ABU43156.1 and 45700 EMBL ABU43154.1.
[2]"Analysis of drug resistance genes of Plasmodium falciparum in isolates from Bikaner, India."
Garg S., Saxena V., Kochar D., Das A.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: INBK1004 EMBL ACG59401.1.
[3]"Trypanosomal dihydrofolate reductase reveals natural antifolate resistance."
Vanichtanankul J., Taweechai S., Yuvaniyama J., Vilaivan T., Chitnumsub P., Kamchonwongpaisan S., Yuthavong Y.
ACS Chem. Biol. 6:905-911(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) IN COMPLEX WITH NADP.
[4]"Combined spatial limitation around residues 16 and 108 of Plasmodium falciparum dihydrofolate reductase explains resistance to cycloguanil."
Vanichtanankul J., Taweechai S., Uttamapinant C., Chitnumsub P., Vilaivan T., Yuthavong Y., Kamchonwongpaisan S.
Antimicrob. Agents Chemother. 56:3928-3935(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) IN COMPLEX WITH NADP AND PHOSPHATE.
[5]"Malarial dihydrofolate reductase as a paradigm for drug development against a resistance-compromised target."
Yuthavong Y., Tarnchompoo B., Vilaivan T., Chitnumsub P., Kamchonwongpaisan S., Charman S.A., McLennan D.N., White K.L., Vivas L., Bongard E., Thongphanchang C., Taweechai S., Vanichtanankul J., Rattanajak R., Arwon U., Fantauzzi P., Yuvaniyama J., Charman W.N., Matthews D.
Proc. Natl. Acad. Sci. U.S.A. 109:16823-16828(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) IN COMPLEX WITH NADP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EU046230 Genomic DNA. Translation: ABU43153.1.
EU046231 Genomic DNA. Translation: ABU43154.1.
EU046232 Genomic DNA. Translation: ABU43155.1.
EU046233 Genomic DNA. Translation: ABU43156.1.
EU682760 Genomic DNA. Translation: ACG59401.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3QGTX-ray2.30A/B1-608[»]
3UM5X-ray2.40A/B1-608[»]
3UM6X-ray2.65A/B1-608[»]
3UM8X-ray2.60A/B1-608[»]
4DPDX-ray2.50A/B1-608[»]
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFPIRSF000389. DHFR-TS. 1 hit.
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceA7UD81.

Entry information

Entry nameA7UD81_PLAFA
AccessionPrimary (citable) accession number: A7UD81
Entry history
Integrated into UniProtKB/TrEMBL: October 2, 2007
Last sequence update: October 2, 2007
Last modified: March 19, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)