Reviewed, UniProtKB/Swiss-Prot A7U6F1 (DPOL_CEV01)

Names and origin

Protein names

Recommended name:
    DNA polymerase
    EC=2.7.7.7
Cleaved into the following chain:
    1- Recommended name:
            CeV01 dpo intein

Gene names

Name:

dpo

Organism

Chrysochromulina ericina virus (CeV01)

Taxonomic identifier

455364 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Phycodnaviridae › unclassified Phycodnaviridae

Virus host

Chrysochromulina ericina [TaxID: 156174]

Protein attributes

Sequence length	1623 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the DNA polymerase type-B family.

Ontologies

Keywords
Biological process	DNA replication
Domain	Repeat
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
PTM	Autocatalytic cleavage Protein splicing
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 964	964	DNA polymerase, 1st part Potential		PRO_0000338005
	Chain	965 – 1335	371	CeV01 dpo intein Potential		PRO_0000338006
	Chain	1336 – 1623	288	DNA polymerase, 2nd part Potential		PRO_0000338007

Sequences

	Sequence		Length	Mass (Da)
	A7U6F1-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 669E878EF1B17322	FASTA	1,623	187,901
	10 20 30 40 50 60 MSQWQFKLFE FDIREELETD KNEFIPGRDT KRFIIQMYGI DENGKTACIF VKGFNPFFYV 70 80 90 100 110 120 KVLDEWDNSK VTEFVAFVRK EMGAYFGDSL VSAKIVYRHK FYEFDNKKLY KFVQLKFTSI 130 140 150 160 170 180 GAFNKCKNLW YNETKYGEDR KLKENGLEFL DTKTTLYEAQ VPPLLRLFHI RQIKPSGWVA 190 200 210 220 230 240 LKNGHYSQNR KQLTTCDYEF TVNYKNIYPI NDERLEKICV PFKILSLDIE ASSSHGDFPL 250 260 270 280 290 300 ARKNYLKLAT NIVDYLINNN IETCDKDLLS NLIKTGFSYR INKDIEKIYL KKSITEEELD 310 320 330 340 350 360 ELIDNLVDIK PGKKENYIDI KDEDENSDSS DIENDGIVEE FTTKKRQKVT GTKNKDSNIL 370 380 390 400 410 420 EIINDLTCNR NTRILELAKC FGQHNPNRDN KWEGKFPELE GDQVTIIGST IRRNGEDKPY 430 440 450 460 470 480 LQHAIVVNDC NSIDNVVIES YKTERDALLA WTNFVQRENP DIIIGYNHHG WDEGFMYDRS 490 500 510 520 530 540 IELNCMTQFS KLSRFKNEKC IKEIFQGKNK PKKITIEESS TKLASGQFDL RYFKMSGRLQ 550 560 570 580 590 600 IDFLNLFRRE EQLPSYKLDY VAGHFIGDNI KKIEYDENCS ILYSKNLTGL SKNDYIVIQE 610 620 630 640 650 660 IGYSTDQYAN GKKFKVLDIV DDKIILNDKI TPDTNKILRW CLGKDDVGPQ DIFRLTNEGP 670 680 690 700 710 720 EGRAIVTKYC IKDCDLVQDL MRKNDTMTSY DEMSNLCWVP KSFLVTRGQG IKLTSYVAQK 730 740 750 760 770 780 CREKNTLMPV IDKGIDGEGY EGAIVLEPKC NLYLKKPVAC VDYSSLYPSS IISENISHDS 790 800 810 820 830 840 KVWTKEYDLD DNLINETGEK DENGKFIYDE LPEYDYIDIT YDTFKWIRKT PKAAATKVKS 850 860 870 880 890 900 GYKTCRYVQF QNNEKAILPS ILDELLGARK STKRQMKNET DPFMQNVLDK RQLSIKITAN 910 920 930 940 950 960 SLYGQAGAKT STFYDKDIAA STTATGRKLL IYAKELIEAC YDNTIEETTN YGEVKCYGEY 970 980 990 1000 1010 1020 IYGDSVASYT PIYVRYNKSI IDICSVEELA EKYGNGWHLE SPKEYCELNN IESWTENGWT 1030 1040 1050 1060 1070 1080 ECHRVIRHRL APYKKMVRIL THTGLVDVTD DHSLVKNTGE EISPKDVSIG TKLLHCTMSE 1090 1100 1110 1120 1130 1140 NESNIESDIS IDEARIMGFF FGDGSCGIYD CPSGHKASWA LNNSNKELIE KYYNLCKSVY 1150 1160 1170 1180 1190 1200 PEFEWKVYDT LNSSGVYKIC FNKKSGSKSK IQFIEKYRSM LYNKKSKIIP SEIINGSIEL 1210 1220 1230 1240 1250 1260 RKSFWEGLYD ADGDKDKNGY TRIDQKSQIS AAYICWLANS IGYKTSLNIR DDKTDIYRIT 1270 1280 1290 1300 1310 1320 ATKNKQRRDG DKIKKIVNIQ NSANIQNSAN IQNSVNIQNS VNIQNSKDNQ DYVYDLTTEN 1330 1340 1350 1360 1370 1380 HHHFAAGIGN MIVHNTDSVF FTFNLKDLDS KEIVGKKALE ITIELAKKAG WLATMFLKSP 1390 1400 1410 1420 1430 1440 HDLEYEKTFL PFCLLSKKRY VGILYEEDPN KGKRKEMGLV LKRRDNCAQV KDVYGGAIDI 1450 1460 1470 1480 1490 1500 LMKDQVVHKA VDFVKHSLQD VIDEKISQQK LIITKSLRSY YKNPKQIAHN VLAIRIGERD 1510 1520 1530 1540 1550 1560 PGNKPKPGDR MEFIYIKNSD KKALQGDRIE TPLFINENNI EIDYGFYITN QIMKPLQQLF 1570 1580 1590 1600 1610 1620 ALVLEDMEDF VTKRGISMKS WKAEIDKLHE KWTEPDKFSK KYEELRCKEV KSILFDPYIK LLK

References

[1]

"Phylogenetic analysis of members of the Phycodnaviridae virus family, using amplified fragments of the major capsid protein gene." Larsen J.B., Larsen A., Bratbak G., Sandaa R.A. Appl. Environ. Microbiol. 74:3048-3057(2008) [PubMed: 18359826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	EU006632 Genomic DNA. Translation: ABU23716.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR006172. DNA-dir_DNA_pol_B. IPR006134. DNA-dir_DNA_pol_B_cons-reg. IPR017966. DNA-dir_DNA_pol_B_cons_reg2. IPR006133. DNA-dir_DNA_pol_B_exonuc. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR012337. PolynucTfrase_RNaseH_fold. [Graphical view]
PANTHER	PTHR10322. DNA_pol_B. 1 hit.
Pfam	PF00136. DNA_pol_B. 1 hit. PF03104. DNA_pol_B_exo. 1 hit. [Graphical view]
PRINTS	PR00106. DNAPOLB. PR00379. INTEIN.
SMART	SM00486. POLBc. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DPOL_CEV01

Accession

Primary (citable) accession number: A7U6F1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	October 2, 2007
Last modified:	November 24, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)