A7TZU4 (ENP1_ENCIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spore wall and anchoring disk complex protein EnP1

Alternative name(s):
Host cell adhesion protein EnP1

Gene names

Name:

EnP1

Organism

Encephalitozoon intestinalis (Microsporidian parasite)

Taxonomic identifier

58839 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Unikaryonidae › Encephalitozoon

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Predicted

General annotation (Comments)

Function	Spore wall protein involved in the adhesion to host cells surface glycoaminoglycans (GAGs). Microsporidian spore adherence is an integral part of activation and host cell infection. Ref.1
Subcellular location	Spore wall. Spore wall › perispore. Note: Localizes also at the anchoring disk complex which consists of the polar sac and the anchoring disk, playing a crucial role in the rupture of the spore wall and the subsequent release of the polar tube following activation. Ref.1
Domain	Heparin-binding motifs (HBM) are characterized by an XBBXBX or XBBBXXBX sequence, where X is any neutral amino acid and B is a positively charged basic amino acid, and are defined as the consensus sequence necessary for protein-heparin interactions. HBM motifs may be involved in spore adherence to host cells. Ref.1

Ontologies

Keywords
Biological process	Cell adhesion Sporulation
Domain	Signal
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	spore wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 348	332	Spore wall and anchoring disk complex protein EnP1		PRO_0000377524
Regions
	Motif	193 – 198	6	HBM1
	Motif	248 – 256	9	HBM2
	Motif	322 – 327	6	HBM3
Amino acid modifications
	Glycosylation	47	1	N-linked (GlcNAc...) Potential
	Glycosylation	139	1	N-linked (GlcNAc...) Potential
	Glycosylation	140	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A7TZU4 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 13694D713A5FA4A2	FASTA	348	39,101
	10 20 30 40 50 60 MKLLGLLISA FGAINALKIK ALYLSCDYEL RPYNAVIDSQ CMAFALNGSN IHEAIRYLNA 70 80 90 100 110 120 MNIDKAYVLY WNDHDLHQNP MVLHKNGALA PFDRYTNTAK HVLCVEACSC PGPQSRPVVC 130 140 150 160 170 180 PENNGASVSS PCPPCGQGNN TTVCDKVVVN PQPVKPLPAP CTPCAPCESS SSEKSESKEC 190 200 210 220 230 240 MTFPRICKKK CGPRHGRSPK KVEIVKSQKT YTFDIERYKR RGDVVVRVCS QDCKDKFEKF 250 260 270 280 290 300 VLTKTGEIRK GKDKKCIPEP LPECLQCPKN LYKLKSGIEA KVCSEVCMYI NSKCEIFVLI 310 320 330 340 GDCDFYKVVM NERRRKQSSF HLKKIRGQKL RELIRQGLFG VEFSPLKC

References

[1]

"EnP1, a microsporidian spore wall protein that enables spores to adhere to and infect host cells in vitro." Southern T.R., Jolly C.E., Lester M.E., Hayman J.R. Eukaryot. Cell 6:1354-1362(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DOMAIN, SUBCELLULAR LOCATION, FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF539266 Genomic DNA. Translation: ABU24317.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

ENP1_ENCIN

Accession

Primary (citable) accession number: A7TZU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	October 2, 2007
Last modified:	January 11, 2011
This is version 6 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program