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Protein

Mannosylfructose-phosphate synthase

Gene

mfpsA

Organism
Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

GDP-mannose + D-fructose 6-phosphate = GDP + beta-D-fructofuranosyl-alpha-D-mannopyranoside 6(F)-phosphate.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 Publication

Kineticsi

  1. KM=1.5 mM for GDP-mannose1 Publication
  2. KM=1.0 mM for fructose-6-phosphate1 Publication

    pH dependencei

    Optimum pH is 8.0.1 Publication

    Pathwayi: mannosylfructose biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes beta-D-fructofuranosyl alpha-D-mannopyranoside from D-fructose 6-phosphate and GDP-alpha-D-mannose.1 Publication
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Mannosylfructose-phosphate synthase (mfpsA)
    2. Mannosylfructose-phosphate phosphatase (mfppA)
    This subpathway is part of the pathway mannosylfructose biosynthesis, which is itself part of Carbohydrate metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes beta-D-fructofuranosyl alpha-D-mannopyranoside from D-fructose 6-phosphate and GDP-alpha-D-mannose, the pathway mannosylfructose biosynthesis and in Carbohydrate metabolism.

    GO - Molecular functioni

    GO - Biological processi

    • disaccharide biosynthetic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciAGRO:ATU0661-MONOMER.
    MetaCyc:MONOMER-14460.
    BRENDAi2.4.1.246. 200.
    UniPathwayiUPA01006; UER01009.

    Protein family/group databases

    CAZyiGT4. Glycosyltransferase Family 4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannosylfructose-phosphate synthaseImported (EC:2.4.1.246)
    Gene namesi
    Name:mfpsAImported
    Ordered Locus Names:Atu0661
    ORF Names:AGR_C_1178
    OrganismiAgrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
    Taxonomic identifieri176299 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
    Proteomesi
    • UP000000813 Componenti: Chromosome circular

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004011021 – 454Mannosylfructose-phosphate synthaseAdd BLAST454

    Expressioni

    Inductioni

    By salt.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi176299.Atu0661.

    Structurei

    3D structure databases

    ProteinModelPortaliA7TZT2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 1 family.Sequence analysis

    Phylogenomic databases

    eggNOGiENOG4107STW. Bacteria.
    COG0438. LUCA.
    HOGENOMiHOG000024913.
    KOiK13058.

    Family and domain databases

    InterProiIPR001296. Glyco_trans_1.
    IPR028098. Glyco_trans_4-like_N.
    [Graphical view]
    PfamiPF13439. Glyco_transf_4. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7TZT2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEKFTKMGPM TTTSETERYP RIALISTHGY VAAHPPLGAA DTGGQVVYVL
    60 70 80 90 100
    ELARKLGQLG YTVDLYTRRF EDQPEFDEVD ERVRVVRIPC GGRDFIPKEY
    110 120 130 140 150
    LHRHLMEWCE NALRFIKKND LNYSFINSHY WDAGVAGQRL SEALKIPHLH
    160 170 180 190 200
    TPHSLGIWKK RQMETDYPEK ADTFELEFNF KERIQHELII YRSCDMVIAT
    210 220 230 240 250
    TPVQLDVLIE DYGLKRKHIH MIPPGYDDNR FFPVSDATRQ MIRQRFGFEG
    260 270 280 290 300
    KVVLALGRLA TNKGYDLLID GFSVLAEREP EARLHLAVGG ENMDEQETTI
    310 320 330 340 350
    LNQLKERVKS LGLEDKVAFS GYVADEDLPD IYRAADLFVL SSRYEPFGMT
    360 370 380 390 400
    AIEAMASGTP TVVTIHGGLF RAISYGRHAL FADPFDKEDL GITMMKPFKH
    410 420 430 440 450
    ERLYGRLSRM GAHKARSLFT WTGIAQQLLA LVEGRTMMPV LEEADWAEPW

    NDGD
    Length:454
    Mass (Da):51,911
    Last modified:January 15, 2008 - v1
    Checksum:iD44776F9FEA75898
    GO

    Sequence cautioni

    The sequence AAK86469 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    EF530045 Genomic DNA. Translation: ABU63292.1.
    AE007869 Genomic DNA. Translation: AAK86469.2. Different initiation.
    RefSeqiNP_353684.2. NC_003062.2.

    Genome annotation databases

    EnsemblBacteriaiAAK86469; AAK86469; Atu0661.
    GeneIDi1132699.
    KEGGiatu:Atu0661.
    PATRICi20811021. VBIAgrTum91616_0654.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    EF530045 Genomic DNA. Translation: ABU63292.1.
    AE007869 Genomic DNA. Translation: AAK86469.2. Different initiation.
    RefSeqiNP_353684.2. NC_003062.2.

    3D structure databases

    ProteinModelPortaliA7TZT2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi176299.Atu0661.

    Protein family/group databases

    CAZyiGT4. Glycosyltransferase Family 4.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAK86469; AAK86469; Atu0661.
    GeneIDi1132699.
    KEGGiatu:Atu0661.
    PATRICi20811021. VBIAgrTum91616_0654.

    Phylogenomic databases

    eggNOGiENOG4107STW. Bacteria.
    COG0438. LUCA.
    HOGENOMiHOG000024913.
    KOiK13058.

    Enzyme and pathway databases

    UniPathwayiUPA01006; UER01009.
    BioCyciAGRO:ATU0661-MONOMER.
    MetaCyc:MONOMER-14460.
    BRENDAi2.4.1.246. 200.

    Family and domain databases

    InterProiIPR001296. Glyco_trans_1.
    IPR028098. Glyco_trans_4-like_N.
    [Graphical view]
    PfamiPF13439. Glyco_transf_4. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMFPS_AGRFC
    AccessioniPrimary (citable) accession number: A7TZT2
    Secondary accession number(s): A9CK29
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: January 15, 2008
    Last modified: September 7, 2016
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.