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A7TTQ1 (MSH3_VANPO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA mismatch repair protein MSH3
Alternative name(s):
MutS protein homolog 3
Gene names
Name:MSH3
ORF Names:Kpol_183p2
OrganismVanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus) [Complete proteome]
Taxonomic identifier436907 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeVanderwaltozyma

Protein attributes

Sequence length1023 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta, which binds to DNA mismatches thereby initiating DNA repair. MSH3 provides substrate-binding and substrate specificity to the complex. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. Acts mainly to repair insertion-deletion loops (IDLs) from 2 to 13 nucleotides in size, but can also repair base-base and single insertion-deletion mismatches that occur during replication. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions By similarity.

Subunit structure

Heterodimer consisting of MSH2-MSH3 (MutS beta). Forms a ternary complex with MutL alpha (MLH1-PMS1) By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the DNA mismatch repair MutS family. MSH3 subfamily.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
   Cellular componentNucleus
   LigandATP-binding
DNA-binding
Nucleotide-binding
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmismatch repair

Inferred from electronic annotation. Source: InterPro

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

mismatched DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10231023DNA mismatch repair protein MSH3
PRO_0000338532

Regions

Nucleotide binding792 – 7998ATP Potential
Region129 – 259131Mispair-binding domain By similarity

Sequences

Sequence LengthMass (Da)Tools
A7TTQ1 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: 2A1A7FF99F8FA1D4

FASTA1,023117,059
        10         20         30         40         50         60 
MSKQPVISRF FKPIARKEVT SEKGVEKEKP VDLDEEKHNE PATTSRLMPK GFDAAAETVT 

        70         80         90        100        110        120 
DEVIEIEVEI EHESEPKIEI KNGTVTAENK SMDFAEKLNR IWNDKKRVIS NNDNDDSDGE 

       130        140        150        160        170        180 
NDTIVKKSKN NSNKLTPLDQ QVKDLKLLHM DKILVIRVGY KYKCFAQDAE IVSKILHIML 

       190        200        210        220        230        240 
IPGKLTIDES NPQDSNYRQF AYCSFPDIRL KVHLETLVHN NLKVAVVEQS ETSAIKKNSN 

       250        260        270        280        290        300 
ASSKNSVFER KISGVYTKAT FGINSAFSSN RKNVLGQYNS IWIINFSEID KINSSFFMIS 

       310        320        330        340        350        360 
VNLNSGEIIY DTFECSTTSI ENLETRIKYL NPIEVLTVSA LPEKVKLRLH GSNSTILLKE 

       370        380        390        400        410        420 
KEDIDKEIME EINKSTKGLN LSAELFELVP VLYKYLTEYN NEELLLISEN YKPFASKKHM 

       430        440        450        460        470        480 
VLNAAAIESL GIFGEEGGKG SLFWLLDHTR TSFGSRKLRE WILHPLLDKK EIEDRLDAVD 

       490        500        510        520        530        540 
CIIHEVSNIF FESLNKMLTN VPDLLRTINR IAYGTTSRKE IYYFLKQMKS FSDHFQLHSN 

       550        560        570        580        590        600 
YLNSQVVSND GRIHKSSALL TNLLTEITSG LKEINIENIL SMINVSSVME KDTYKQVSEF 

       610        620        630        640        650        660 
FNLNYYDHAE EIIKIQGNIN EVKNELAEEL SSIRKILKRP HLNYKDEMDY LIEVRNTQTK 

       670        680        690        700        710        720 
GLPSDWIVVN RTKMISRYHT PTSRKLIEKL QYQKDILYQE TQKEYFQFVK RIKNDYFALN 

       730        740        750        760        770        780 
KIINHIATYD CILALASTSQ NMNYVRPVLT DESQFIDAKN ARNPIIESLD INYVPNDVNL 

       790        800        810        820        830        840 
SHSAGKFLII TGPNMGGKSS YIRQVALLVI LAQVGSYVPA DFMKTSIFDK ILTRIGAYDN 

       850        860        870        880        890        900 
LLKGQSTFKV ELLEIQNIIK NKTENSLLLL DEVGRGTSTE DGKAISYSIV DYFINLPVCP 

       910        920        930        940        950        960 
LVLFTTHYPF LGSINSKILK SYYMDFVEQK KEGEDWPSIV FLYKLRSGIT DSSFGLNVAR 

       970        980        990       1000       1010       1020 
LAQIDKDIIN HAFSISEKIR QETETANTMN LPILLKHILS SNDLKPQQKI IEILNLQNDS 


QEL 

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References

[1]"Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication."
Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H.
Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 22028 / DSM 70294.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS480605 Genomic DNA. Translation: EDO14357.1.
RefSeqXP_001642215.1. XM_001642165.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING436907.A7TTQ1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5542335.
KEGGvpo:Kpol_183p2.

Phylogenomic databases

eggNOGCOG0249.
KOK08736.
OrthoDBEOG773XQH.

Family and domain databases

Gene3D3.40.1170.10. 1 hit.
3.40.50.300. 1 hit.
InterProIPR007695. DNA_mismatch_repair_MutS-lik_N.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007861. DNA_mismatch_repair_MutS_clamp.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR016151. DNA_mismatch_repair_MutS_N.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01624. MutS_I. 1 hit.
PF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF05190. MutS_IV. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
SMARTSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMSSF48334. SSF48334. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55271. SSF55271. 1 hit.
PROSITEPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMSH3_VANPO
AccessionPrimary (citable) accession number: A7TTQ1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: October 2, 2007
Last modified: June 11, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families