A7TTQ1 (MSH3_VANPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA mismatch repair protein MSH3

Alternative name(s):
MutS protein homolog 3

Gene names

Name:	MSH3
ORF Names:	Kpol_183p2

Organism

Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus) [Complete proteome]

Taxonomic identifier

436907 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Vanderwaltozyma › Vanderwaltozyma polyspora

Protein attributes

Sequence length	1023 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta, which binds to DNA mismatches thereby initiating DNA repair. MSH3 provides substrate-binding and substrate-specificity to the complex. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. Acts mainly to repair insertion-deletion loops (IDLs) from 2 to 13 nucleotides in size, but can also repair base-base and single insertion-deletion mismatches that occur during replication. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions By similarity.
Subunit structure	Heterodimer consisting of MSH2-MSH3 (MutS beta). Forms a ternary complex with MutL alpha (MLH1-PMS1) By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the DNA mismatch repair MutS family. MSH3 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mismatch repair Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW mismatched DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1023	1023	DNA mismatch repair protein MSH3		PRO_0000338532
Regions
	Nucleotide binding	792 – 799	8	ATP Potential
	Region	129 – 259	131	Mispair-binding domain By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7TTQ1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 2A1A7FF99F8FA1D4	FASTA	1,023	117,059
	10 20 30 40 50 60 MSKQPVISRF FKPIARKEVT SEKGVEKEKP VDLDEEKHNE PATTSRLMPK GFDAAAETVT 70 80 90 100 110 120 DEVIEIEVEI EHESEPKIEI KNGTVTAENK SMDFAEKLNR IWNDKKRVIS NNDNDDSDGE 130 140 150 160 170 180 NDTIVKKSKN NSNKLTPLDQ QVKDLKLLHM DKILVIRVGY KYKCFAQDAE IVSKILHIML 190 200 210 220 230 240 IPGKLTIDES NPQDSNYRQF AYCSFPDIRL KVHLETLVHN NLKVAVVEQS ETSAIKKNSN 250 260 270 280 290 300 ASSKNSVFER KISGVYTKAT FGINSAFSSN RKNVLGQYNS IWIINFSEID KINSSFFMIS 310 320 330 340 350 360 VNLNSGEIIY DTFECSTTSI ENLETRIKYL NPIEVLTVSA LPEKVKLRLH GSNSTILLKE 370 380 390 400 410 420 KEDIDKEIME EINKSTKGLN LSAELFELVP VLYKYLTEYN NEELLLISEN YKPFASKKHM 430 440 450 460 470 480 VLNAAAIESL GIFGEEGGKG SLFWLLDHTR TSFGSRKLRE WILHPLLDKK EIEDRLDAVD 490 500 510 520 530 540 CIIHEVSNIF FESLNKMLTN VPDLLRTINR IAYGTTSRKE IYYFLKQMKS FSDHFQLHSN 550 560 570 580 590 600 YLNSQVVSND GRIHKSSALL TNLLTEITSG LKEINIENIL SMINVSSVME KDTYKQVSEF 610 620 630 640 650 660 FNLNYYDHAE EIIKIQGNIN EVKNELAEEL SSIRKILKRP HLNYKDEMDY LIEVRNTQTK 670 680 690 700 710 720 GLPSDWIVVN RTKMISRYHT PTSRKLIEKL QYQKDILYQE TQKEYFQFVK RIKNDYFALN 730 740 750 760 770 780 KIINHIATYD CILALASTSQ NMNYVRPVLT DESQFIDAKN ARNPIIESLD INYVPNDVNL 790 800 810 820 830 840 SHSAGKFLII TGPNMGGKSS YIRQVALLVI LAQVGSYVPA DFMKTSIFDK ILTRIGAYDN 850 860 870 880 890 900 LLKGQSTFKV ELLEIQNIIK NKTENSLLLL DEVGRGTSTE DGKAISYSIV DYFINLPVCP 910 920 930 940 950 960 LVLFTTHYPF LGSINSKILK SYYMDFVEQK KEGEDWPSIV FLYKLRSGIT DSSFGLNVAR 970 980 990 1000 1010 1020 LAQIDKDIIN HAFSISEKIR QETETANTMN LPILLKHILS SNDLKPQQKI IEILNLQNDS QEL

References

[1]

"Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication." Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H. Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 22028 / DSM 70294.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS480605 Genomic DNA. Translation: EDO14357.1.
RefSeq	XP_001642215.1. XM_001642165.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	436907.A7TTQ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5542335.
KEGG	vpo:Kpol_183p2.
Phylogenomic databases
eggNOG	COG0249.
KO	K08736.
OrthoDB	EOG42NN7M.
Family and domain databases
Gene3D	3.40.1170.10. 1 hit.
InterPro	IPR007695. DNA_mismatch_repair_MutS-lik_N. IPR000432. DNA_mismatch_repair_MutS_C. IPR007861. DNA_mismatch_repair_MutS_clamp. IPR007696. DNA_mismatch_repair_MutS_core. IPR016151. DNA_mismatch_repair_MutS_N. IPR007860. DNA_mmatch_repair_MutS_con_dom. [Graphical view]
Pfam	PF01624. MutS_I. 1 hit. PF05188. MutS_II. 1 hit. PF05192. MutS_III. 1 hit. PF05190. MutS_IV. 1 hit. PF00488. MutS_V. 1 hit. [Graphical view]
SMART	SM00534. MUTSac. 1 hit. SM00533. MUTSd. 1 hit. [Graphical view]
SUPFAM	SSF55271. DNA_mismatch_repair_MutS_N. 1 hit. SSF48334. DNA_repair_MutS_domIII. 1 hit.
PROSITE	PS00486. DNA_MISMATCH_REPAIR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MSH3_VANPO

Accession

Primary (citable) accession number: A7TTQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	October 2, 2007
Last modified:	April 3, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program