A7TT36 (TRMB_VANPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	TRM8
ORF Names:	Kpol_269p6

Organism

Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus) [Complete proteome]

Taxonomic identifier

436907 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Vanderwaltozyma › Vanderwaltozyma polyspora

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subunit structure	Forms a complex with TRM82 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000370605
Regions
	Region	126 – 127	2	S-adenosyl-L-methionine binding By similarity
	Region	161 – 162	2	S-adenosyl-L-methionine binding By similarity
	Region	259 – 261	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	184	1	By similarity
	Binding site	103	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	181	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7TT36 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 2BBAED087EC7B1D7	FASTA	286	33,804
	10 20 30 40 50 60 MDLRNPNRDP NSRRVLYRTN KEENRKELKH VKIDESTLAQ EGKKLDLPKK RFYRQRAHSN 70 80 90 100 110 120 PFSDHQLDYP TSPDDMNWSK LFPHYYDSTT GKMTKDVTIA DIGCGFGGLL IDLSPAFPED 130 140 150 160 170 180 LILGMEIRVQ VTNYVEDRII ALRTNHAKDY QYQNINVIRG NAMKFLPNFF QRAQLSKMFF 190 200 210 220 230 240 CFPDPHFKQR KHKARIITNT LLSEYAYVLK DNGVIYTITD VEDLHNWMVK HLEEHPLFER 250 260 270 280 YDKEWEDNDK CVQIMRNATE EGKKVERKKG DKFVACFRRL PNPAIV

References

[1]

"Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication." Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H. Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 22028 / DSM 70294.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS480540 Genomic DNA. Translation: EDO14572.1.
RefSeq	XP_001642430.1. XM_001642380.1.
3D structure databases
ProteinModelPortal	A7TT36.
SMR	A7TT36. Positions 60-286.
ModBase	Search...
Protein-protein interaction databases
STRING	436907.A7TT36.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5542586.
KEGG	vpo:Kpol_269p6.
Phylogenomic databases
eggNOG	COG0220.
KO	K03439.
OrthoDB	EOG4M3DJF.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_VANPO

Accession

Primary (citable) accession number: A7TT36

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	October 2, 2007
Last modified:	April 3, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program