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A7TT36

- TRMB_VANPO

UniProt

A7TT36 - TRMB_VANPO

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
TRM8, Kpol_269p6
Organism
Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei103 – 1031S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei181 – 1811S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei184 – 1841 By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:TRM8
ORF Names:Kpol_269p6
OrganismiVanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus)
Taxonomic identifieri436907 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeVanderwaltozyma
ProteomesiUP000000267: Unassembled WGS sequence

Subcellular locationi

Nucleus By similarity UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
PRO_0000370605Add
BLAST

Interactioni

Subunit structurei

Forms a complex with TRM82 By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi436907.A7TT36.

Structurei

3D structure databases

ProteinModelPortaliA7TT36.
SMRiA7TT36. Positions 60-286.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1272S-adenosyl-L-methionine binding By similarity
Regioni161 – 1622S-adenosyl-L-methionine binding By similarity
Regioni259 – 2613S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0220.
KOiK03439.
OrthoDBiEOG708W9T.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7TT36-1 [UniParc]FASTAAdd to Basket

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MDLRNPNRDP NSRRVLYRTN KEENRKELKH VKIDESTLAQ EGKKLDLPKK    50
RFYRQRAHSN PFSDHQLDYP TSPDDMNWSK LFPHYYDSTT GKMTKDVTIA 100
DIGCGFGGLL IDLSPAFPED LILGMEIRVQ VTNYVEDRII ALRTNHAKDY 150
QYQNINVIRG NAMKFLPNFF QRAQLSKMFF CFPDPHFKQR KHKARIITNT 200
LLSEYAYVLK DNGVIYTITD VEDLHNWMVK HLEEHPLFER YDKEWEDNDK 250
CVQIMRNATE EGKKVERKKG DKFVACFRRL PNPAIV 286
Length:286
Mass (Da):33,804
Last modified:October 2, 2007 - v1
Checksum:i2BBAED087EC7B1D7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DS480540 Genomic DNA. Translation: EDO14572.1.
RefSeqiXP_001642430.1. XM_001642380.1.

Genome annotation databases

GeneIDi5542586.
KEGGivpo:Kpol_269p6.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DS480540 Genomic DNA. Translation: EDO14572.1 .
RefSeqi XP_001642430.1. XM_001642380.1.

3D structure databases

ProteinModelPortali A7TT36.
SMRi A7TT36. Positions 60-286.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 436907.A7TT36.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5542586.
KEGGi vpo:Kpol_269p6.

Phylogenomic databases

eggNOGi COG0220.
KOi K03439.
OrthoDBi EOG708W9T.

Enzyme and pathway databases

UniPathwayi UPA00989 .

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication."
    Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H.
    Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 22028 / DSM 70294.

Entry informationi

Entry nameiTRMB_VANPO
AccessioniPrimary (citable) accession number: A7TT36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 2, 2007
Last modified: June 11, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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