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Protein

Mitochondrial intermediate peptidase

Gene

OCT1

Organism
Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane (By similarity).By similarity

Catalytic activityi

Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi573 – 5731Zinc; catalyticPROSITE-ProRule annotation
Active sitei574 – 5741PROSITE-ProRule annotation
Metal bindingi577 – 5771Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi580 – 5801Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial intermediate peptidase (EC:3.4.24.59)
Short name:
MIP
Alternative name(s):
Octapeptidyl aminopeptidase
Gene namesi
Name:OCT1
ORF Names:Kpol_297p8
OrganismiVanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus)
Taxonomic identifieri436907 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeVanderwaltozyma
ProteomesiUP000000267: Unassembled WGS sequence

Subcellular locationi

Mitochondrion matrix By similarity

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3636MitochondrionSequence AnalysisAdd
BLAST
Chaini37 – 787751Mitochondrial intermediate peptidasePRO_0000338596Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi436907.A7TSL2.

Structurei

3D structure databases

ProteinModelPortaliA7TSL2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M3 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0339.
InParanoidiA7TSL2.
KOiK01410.
OrthoDBiEOG71GB4R.

Family and domain databases

Gene3Di1.10.1370.10. 2 hits.
3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamiPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A7TSL2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNKVLRGIL FKNVPLGYSY NRSIRHPTFG NSIIRWASTQ VKTSSDVLQR
60 70 80 90 100
SFDDHLYWTE INKQNYSSKE GWGSITKRLK GNKLTTNRSG LFNNEYLTSP
110 120 130 140 150
EGLKLFSQVS LEKSQKIVDK LRSDRTPEGL RLYVQNLDLL SDTLCRVIDL
160 170 180 190 200
CEFIRSSHPD YKFVEAAQDC YEEMFEFMNM LNTDVNLCFT LKHVLENKEI
210 220 230 240 250
ASKLSEEELR VGRILLEDFE KSGIYMKPEV REQFITLSQS ISVIGQEFIS
260 270 280 290 300
NTDFVKDNNV VVSCNQLDSL GIDPELLSQI EKDIAGKNYK IPTYGYIPFA
310 320 330 340 350
LLKSCPSEEI REKIWVAVHN CSNEQIKRLT DLVKLRAVLS QLLGKKSYAE
360 370 380 390 400
YQLEGKMAKN PKEVIEFIKT LMDFTKPMAA KELDGIAEKK LTIKSNGSNL
410 420 430 440 450
SVCDILKTVR PWDRDYYSAI EREQTSAKNL YGSEEVLKYF TLGNVMQGLS
460 470 480 490 500
NLFQKIYGIK LELDVPKIGE TWSPEVRKIN VISEDEGLIG IIYCDLFERS
510 520 530 540 550
GKTSNAAHFT ICCSRDISPY ETEDSTTQIA IDSKGTRFQL PIISLVCNFS
560 570 580 590 600
KTMISETDSV CFLHLPEVET LFHEMGHAMH SMLGRTKLQN ISGTRCATDF
610 620 630 640 650
VELPSILMEY FARDPRVLET IGKHYLTKET VKREMLEPHL QDLKYLQHCE
660 670 680 690 700
TYSQAKMAML DQTLHGETIS SHLDHLDVVK LYQDLERQLG VLVDDKSNWC
710 720 730 740 750
GKFGHLFGYS AVYYSYLFDR AIASKIWGAL FERNPFSRAS GDKYRNSVLQ
760 770 780
WGGSRDPWHC IASALDKPEL AKGDDEAIKY IGSTNQL
Length:787
Mass (Da):90,031
Last modified:October 2, 2007 - v1
Checksum:i478D339F977CF096
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS480515 Genomic DNA. Translation: EDO14747.1.
RefSeqiXP_001642605.1. XM_001642555.1.

Genome annotation databases

GeneIDi5542774.
KEGGivpo:Kpol_297p8.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS480515 Genomic DNA. Translation: EDO14747.1.
RefSeqiXP_001642605.1. XM_001642555.1.

3D structure databases

ProteinModelPortaliA7TSL2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi436907.A7TSL2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi5542774.
KEGGivpo:Kpol_297p8.

Phylogenomic databases

eggNOGiCOG0339.
InParanoidiA7TSL2.
KOiK01410.
OrthoDBiEOG71GB4R.

Family and domain databases

Gene3Di1.10.1370.10. 2 hits.
3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamiPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication."
    Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H.
    Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 22028 / DSM 70294.

Entry informationi

Entry nameiPMIP_VANPO
AccessioniPrimary (citable) accession number: A7TSL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: October 2, 2007
Last modified: January 7, 2015
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.