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Protein

Mitochondrial intermediate peptidase

Gene

OCT1

Organism
Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane (By similarity).By similarity

Catalytic activityi

Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi573Zinc; catalyticPROSITE-ProRule annotation1
Active sitei574PROSITE-ProRule annotation1
Metal bindingi577Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi580Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM03.006

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial intermediate peptidase (EC:3.4.24.59)
Short name:
MIP
Alternative name(s):
Octapeptidyl aminopeptidase
Gene namesi
Name:OCT1
ORF Names:Kpol_297p8
OrganismiVanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus)
Taxonomic identifieri436907 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeVanderwaltozyma
Proteomesi
  • UP000000267 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 36MitochondrionSequence analysisAdd BLAST36
ChainiPRO_000033859637 – 787Mitochondrial intermediate peptidaseAdd BLAST751

Interactioni

Protein-protein interaction databases

STRINGi436907.XP_001642605.1

Structurei

3D structure databases

ProteinModelPortaliA7TSL2
SMRiA7TSL2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M3 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2090 Eukaryota
COG0339 LUCA
InParanoidiA7TSL2
KOiK01410
OrthoDBiEOG092C0JU2
PhylomeDBiA7TSL2

Family and domain databases

CDDicd06457 M3A_MIP, 1 hit
Gene3Di1.10.1370.10, 2 hits
InterProiView protein in InterPro
IPR033851 M3A_MIP
IPR024077 Neurolysin/TOP_dom2
IPR001567 Pept_M3A_M3B
PANTHERiPTHR11804:SF5 PTHR11804:SF5, 1 hit
PfamiView protein in Pfam
PF01432 Peptidase_M3, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A7TSL2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNKVLRGIL FKNVPLGYSY NRSIRHPTFG NSIIRWASTQ VKTSSDVLQR
60 70 80 90 100
SFDDHLYWTE INKQNYSSKE GWGSITKRLK GNKLTTNRSG LFNNEYLTSP
110 120 130 140 150
EGLKLFSQVS LEKSQKIVDK LRSDRTPEGL RLYVQNLDLL SDTLCRVIDL
160 170 180 190 200
CEFIRSSHPD YKFVEAAQDC YEEMFEFMNM LNTDVNLCFT LKHVLENKEI
210 220 230 240 250
ASKLSEEELR VGRILLEDFE KSGIYMKPEV REQFITLSQS ISVIGQEFIS
260 270 280 290 300
NTDFVKDNNV VVSCNQLDSL GIDPELLSQI EKDIAGKNYK IPTYGYIPFA
310 320 330 340 350
LLKSCPSEEI REKIWVAVHN CSNEQIKRLT DLVKLRAVLS QLLGKKSYAE
360 370 380 390 400
YQLEGKMAKN PKEVIEFIKT LMDFTKPMAA KELDGIAEKK LTIKSNGSNL
410 420 430 440 450
SVCDILKTVR PWDRDYYSAI EREQTSAKNL YGSEEVLKYF TLGNVMQGLS
460 470 480 490 500
NLFQKIYGIK LELDVPKIGE TWSPEVRKIN VISEDEGLIG IIYCDLFERS
510 520 530 540 550
GKTSNAAHFT ICCSRDISPY ETEDSTTQIA IDSKGTRFQL PIISLVCNFS
560 570 580 590 600
KTMISETDSV CFLHLPEVET LFHEMGHAMH SMLGRTKLQN ISGTRCATDF
610 620 630 640 650
VELPSILMEY FARDPRVLET IGKHYLTKET VKREMLEPHL QDLKYLQHCE
660 670 680 690 700
TYSQAKMAML DQTLHGETIS SHLDHLDVVK LYQDLERQLG VLVDDKSNWC
710 720 730 740 750
GKFGHLFGYS AVYYSYLFDR AIASKIWGAL FERNPFSRAS GDKYRNSVLQ
760 770 780
WGGSRDPWHC IASALDKPEL AKGDDEAIKY IGSTNQL
Length:787
Mass (Da):90,031
Last modified:October 2, 2007 - v1
Checksum:i478D339F977CF096
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS480515 Genomic DNA Translation: EDO14747.1
RefSeqiXP_001642605.1, XM_001642555.1

Genome annotation databases

EnsemblFungiiEDO14747; EDO14747; Kpol_297p8
GeneIDi5542774
KEGGivpo:Kpol_297p8

Similar proteinsi

Entry informationi

Entry nameiPMIP_VANPO
AccessioniPrimary (citable) accession number: A7TSL2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: October 2, 2007
Last modified: March 28, 2018
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health