Reviewed, UniProtKB/Swiss-Prot A7TNL7 (NCB5R_VANPO)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 1
    EC=1.6.2.2
Alternative name(s):
    Microsomal cytochrome b reductase

Gene names

Name:	CBR1
ORF Names:	Kpol_1070p17

Organism

Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus) [Complete proteome]

Taxonomic identifier

436907 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Vanderwaltozyma

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Electron donor reductase for cytochrome b5. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	NADH-cytochrome b5 reductase 1		PRO_0000330165
Regions
	Transmembrane	8 – 25	18	Potential
	Domain	41 – 145	105	FAD-binding FR-type
	Nucleotide binding	125 – 140	16	FAD By similarity
	Nucleotide binding	151 – 183	33	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7TNL7-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 99871ED9960C2AC7	FASTA	285	31,346
	10 20 30 40 50 60 MAESMNKIFI AIIAIAVAAA ATKMFTSEEP KSKALPVLVK GEYMQFPLVS IKKLNRNSAI 70 80 90 100 110 120 YRFKLPTDEH VLGLPIGQHI TIKAHIDGSE VVRSYTPISL DSEAKGYFEL LIKSYEQGKI 130 140 150 160 170 180 SKMFTSLKIG DTIDVQGPKG FYEYTDRSSK HLAMIAGGSG LTPMYQIIKS IAENPKDKTK 190 200 210 220 230 240 VTFIYGNVEE IDILLRDDLD KFAASKPGQI TIHYLLDKPS ENWKGGSGYV TPELMKEKLP 250 260 270 280 APADGVQLLV CGPLPMVSAI KRSAVALGFP KAKPVSKMND QVFVF

References

[1]

"Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication." Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H. Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed: 17494770] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DS480432 Genomic DNA. Translation: EDO16134.1.
RefSeq	XP_001643992.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5544288.
KEGG	vpo:Kpol_1070p17.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCB5R_VANPO

Accession

Primary (citable) accession number: A7TNL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	October 2, 2007
Last modified:	October 13, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)