Reviewed, UniProtKB/Swiss-Prot A7TM72 (MCR1B_VANPO)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 2-B
    EC=1.6.2.2
Alternative name(s):
    Mitochondrial cytochrome b reductase B

Gene names

Name:	MCR1B
ORF Names:	Kpol_529p19

Organism

Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus) [Complete proteome]

Taxonomic identifier

436907 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Vanderwaltozyma

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May mediate the reduction of outer membrane cytochrome b5 By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	NADH-cytochrome b5 reductase 2-B		PRO_0000330193
Regions
	Transmembrane	12 – 32	21	Potential
	Domain	53 – 157	105	FAD-binding FR-type
	Nucleotide binding	160 – 195	36	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7TM72-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 82800A04590324D5	FASTA	306	33,628
	10 20 30 40 50 60 MISSFTSLGS RPLLLSSGIA VTAAAAVYFS TGSRLANEAL HNKTFKGFKG PASTWVDLPL 70 80 90 100 110 120 VKFEDLSHDT RKFTFKLPND DDVSGISPLS FLLARPHGTW SLRGIRPYTP VSLPETQGVI 130 140 150 160 170 180 EFVIKHVPNG GMSSHMFSLK PNDTVSFTGP IVKYEWKQNK FDSVTLLGAG SGITPLYQLM 190 200 210 220 230 240 GSILSNPEDK TKINLFYANK TSDDILLKKE LDEFQQKFSD RVKIHYYLSQ PKTKDIASTG 250 260 270 280 290 300 AKKGFIAKED IESLAPASNE NTHVFVCGPE PFVKAYAGQQ GPLFFQGSFG GILKELGYTK SQVFKV

References

[1]

"Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication." Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H. Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed: 17494770] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DS480420 Genomic DNA. Translation: EDO16639.1.
RefSeq	XP_001644497.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5544798.
KEGG	vpo:Kpol_529p19.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCR1B_VANPO

Accession

Primary (citable) accession number: A7TM72

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	October 2, 2007
Last modified:	October 13, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)