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Protein

Histone-lysine N-methyltransferase, H3 lysine-79 specific

Gene

Kpol_1004p44

Organism
Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei479 – 4791S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

  1. histone methyltransferase activity (H3-K79 specific) Source: InterPro

GO - Biological processi

  1. chromatin silencing at telomere Source: InterPro
  2. DNA damage checkpoint Source: InterPro
  3. DNA repair Source: InterPro
  4. regulation of cell cycle Source: InterPro
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulatorUniRule annotation, MethyltransferaseUniRule annotation, Transferase

Keywords - Biological processi

Transcription, Transcription regulationUniRule annotation

Keywords - Ligandi

S-adenosyl-L-methionineUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-79 specificUniRule annotation (EC:2.1.1.43UniRule annotation)
Alternative name(s):
Histone H3-K79 methyltransferaseUniRule annotation
Gene namesi
ORF Names:Kpol_1004p44Imported
OrganismiVanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus)Imported
Taxonomic identifieri436907 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeVanderwaltozyma
ProteomesiUP000000267: Unassembled WGS sequence

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

NucleusUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi436907.A7TJA0.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni429 – 4324S-adenosyl-L-methionine bindingUniRule annotation
Regioni452 – 46110S-adenosyl-L-methionine bindingUniRule annotation
Regioni516 – 5172S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.UniRule annotation

Phylogenomic databases

eggNOGiNOG294902.
InParanoidiA7TJA0.
KOiK11427.
OrthoDBiEOG7KH9VN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR021162. Dot1.
IPR013110. DOT1_dom_Pfam.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF08123. DOT1. 1 hit.
[Graphical view]
PIRSFiPIRSF017570. Histone_H3-K79_MeTrfase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

A7TJA0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSSVFSNNS GSVSVETSVE ISDSRVEGIH KVSKGSSGVQ SLLEDAYRYS
60 70 80 90 100
AHSDYTLPGG FLRQRKPPKR FVDSSDDEGD GPTDKKVVKI NQKKSMLSNG
110 120 130 140 150
VKSKSILNSG KSIDGLKKKP NGKDKKNGKV TSIEKTVKSN SKSKSESKLK
160 170 180 190 200
TNLKTKLKTK AELKSKSKSK STADSKPVSK LESNATSSKA KYTKKNKKSP
210 220 230 240 250
EPALLNNSVN GDSPNDSENT NNDNENKVIN DVPVTPSSFA DLTSPFFEFK
260 270 280 290 300
YKFFDIDYLK SNDRFKGNIT QSISITEKYY KSKGDNIIPK FVKLKYPLFL
310 320 330 340 350
NYEEEYHIDF SNESFSTIYN PMSEIGKIIE YCTEIYIPDQ FLKQLETEVI
360 370 380 390 400
KPLNETFDNS DELGFINTVN RYNDIIEKIP RDLMIEKLNN TKSIPKNFIH
410 420 430 440 450
DFLHIIYTRT IHPKANKLRQ YAAFSSFVYG ELLPSFLSEV YSECSLKEGD
460 470 480 490 500
VFMDLGSGVG NCVVQAALEY GCGLSFGCEI MENASDLTEA QFAELQQRAR
510 520 530 540 550
LFGIRLSPVE YSLRESFVDN KRVDELIKKC DVLLVNNFLF DSKLNLEVEK
560 570 580 590 600
IIQNCKSGCK IISLKSLRPL TYTIDFDDIE NILNRLSVKK HKLKENSVSW
610 620 630
THRGGEFYIS TVLSDVDDSL FTSNAGHRRN ERLKYTR
Length:637
Mass (Da):72,008
Last modified:October 2, 2007 - v1
Checksum:iCF048C6CC08E914E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS480400 Genomic DNA. Translation: EDO17669.1.
RefSeqiXP_001645527.1. XM_001645477.1.

Genome annotation databases

GeneIDi5545909.
KEGGivpo:Kpol_1004p44.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS480400 Genomic DNA. Translation: EDO17669.1.
RefSeqiXP_001645527.1. XM_001645477.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi436907.A7TJA0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi5545909.
KEGGivpo:Kpol_1004p44.

Phylogenomic databases

eggNOGiNOG294902.
InParanoidiA7TJA0.
KOiK11427.
OrthoDBiEOG7KH9VN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR021162. Dot1.
IPR013110. DOT1_dom_Pfam.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF08123. DOT1. 1 hit.
[Graphical view]
PIRSFiPIRSF017570. Histone_H3-K79_MeTrfase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication."
    Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H.
    Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 22028 / DSM 70294Imported.

Entry informationi

Entry nameiA7TJA0_VANPO
AccessioniPrimary (citable) accession number: A7TJA0
Entry historyi
Integrated into UniProtKB/TrEMBL: October 2, 2007
Last sequence update: October 2, 2007
Last modified: March 4, 2015
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.