Reviewed, UniProtKB/Swiss-Prot A7TIZ4 (ATG1_VANPO)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase atg1
    EC=2.7.11.1
Alternative name(s):
    Autophagy-related protein 1

Gene names

Name:	ATG1
ORF Names:	Kpol_541p49

Organism

Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus) [Complete proteome]

Taxonomic identifier

436907 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Vanderwaltozyma

Protein attributes

Sequence length	994 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Serine/threonine protein kinase probably involved in the cytoplasm to vacuole transport (Cvt) and in autophagy, where it may be required for the formation of autophagosomes By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 994	994	Serine/threonine-protein kinase atg1		PRO_0000317799
Regions
	Domain	7 – 331	325	Protein kinase
	Nucleotide binding	13 – 21	9	ATP By similarity
	Compositional bias	377 – 525	149	Asn-rich
Sites
	Active site	178	1	Proton acceptor By similarity
	Binding site	60	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7TIZ4-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 9BD130D2C810CE81	FASTA	994	113,085
	10 20 30 40 50 60 MIISGKYVIE KEIGKGSFAT VLKGYIIDDN DNNGNDTNNE DVEVNDDKRK YTTRNQIAVK 70 80 90 100 110 120 AVPRSKLKNK KLLENLEVEI AILKKIKHPH IVRLIECERT STDFYLIMEY CALGDLTFLI 130 140 150 160 170 180 KKRQEIMENH PLLKSVFKRF PPPSKNHNGL HRAFILNYLQ QLSSSLKFLR SKNLVHRDIK 190 200 210 220 230 240 PQNLLLATPF VDYHDSKSFH DLGYVGISSL PILKIADFGF ARFLPNTSMA ETLCGSPLYM 250 260 270 280 290 300 APEILNYQKY NAKADLWSVG TVLYEMCYGK PPFKASNHLE LYKKIKKANN TISYSNDCEI 310 320 330 340 350 360 EDDLKDLINA LLTFDPNKRI GFQEFFDNKL VIEDLSKYEV ANEVYSELEL KSKSVLESNM 370 380 390 400 410 420 FISEYLPSEK KKDDTDNMII SNLSTNDRIN NITIFSNILP TVNENENDKT NDNSTTNYNN 430 440 450 460 470 480 NDNENNDNNN TNHNYNNDKV NNKSNDNNEK SDINRSNSNY KQHSTKQTTN ELIPNTNTIT 490 500 510 520 530 540 TNINDNSNSE MNSQSARMMQ KSKNYKSLRN NKLRNSKNDI INNSNSDLIL EKEYVVVEKK 550 560 570 580 590 600 SVEVNTLADE MAQIGQINNL NYKVPYPSRS PTLAGNVLQN NESLQHYQPF SVKSPRTSLS 610 620 630 640 650 660 SNGSGNSRRA SLVDRRLSIS SLNPSNALSR ALGVASTKLF GSNVLPNQNA GVGNANNINT 670 680 690 700 710 720 SMPLLNPQVF QDLTENILLR VDQLQGKDRI NIDSNSIVQI LESLAAKAFV VHSFAEVKFS 730 740 750 760 770 780 QTIPLKSLST STVNRPANDR RLNDRGCAIE EDDNIQTETY DTYIPSLKGR SLSSLSQTSI 790 800 810 820 830 840 CSQDLPHGEL YQLSKEAIIL YMKTLSILAT AMQITSNWWY QSNEKNCSLR LNLLVQWIRD 850 860 870 880 890 900 KFNECLERAD FLRIQILELE ECENKSNELN SDHTDVETSG VNETVHNNDN DANEEDQIYV 910 920 930 940 950 960 EKLLYDRALE ISRTAAKLEI QGDHLNNCEL AYATSLWMLE ILLDDRIEDD NFNEFSSKNS 970 980 990 AVLDDQDREI IKKYIDSIAN RLKALREKIS QGGS

References

[1]

"Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication." Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H. Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed: 17494770] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DS480398 Genomic DNA. Translation: EDO17806.1.
RefSeq	XP_001645664.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5546060.
KEGG	vpo:Kpol_541p49.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG1_VANPO

Accession

Primary (citable) accession number: A7TIZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 2, 2007
Last modified:	June 16, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)