ID   MCR1A_VANPO             Reviewed;         296 AA.
AC   A7THS1;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   16-JUN-2009, entry version 15.
DE   RecName: Full=NADH-cytochrome b5 reductase 2-A;
DE            EC=1.6.2.2;
DE   AltName: Full=Mitochondrial cytochrome b reductase A;
GN   Name=MCR1A; ORFNames=Kpol_543p67;
OS   Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294)
OS   (Kluyveromyces polysporus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
OC   Vanderwaltozyma.
OX   NCBI_TaxID=436907;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17494770; DOI=10.1073/pnas.0608218104;
RA   Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M.,
RA   Wolfe K.H.;
RT   "Independent sorting-out of thousands of duplicated gene pairs in two
RT   yeast species descended from a whole-genome duplication.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007).
CC   -!- FUNCTION: May mediate the reduction of outer membrane cytochrome
CC       b5 (By similarity).
CC   -!- CATALYTIC ACTIVITY: NADH + 2 ferricytochrome b5 = NAD(+) + H(+) +
CC       2 ferrocytochrome b5.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass
CC       membrane protein (By similarity).
CC   -!- SIMILARITY: Belongs to the flavoprotein pyridine nucleotide
CC       cytochrome reductase family.
CC   -!- SIMILARITY: Contains 1 FAD-binding FR-type domain.
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DR   EMBL; DS480392; EDO18237.1; -; Genomic_DNA.
DR   RefSeq; XP_001646095.1; -.
DR   GeneID; 5546512; -.
DR   KEGG; vpo:Kpol_543p67; -.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004128; F:cytochrome-b5 reductase activity; IEA:EC.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR017927; Fd_Rdtase_FAD-bd.
DR   InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
DR   InterPro; IPR001834; NADH-Cyt_B5_reductase.
DR   InterPro; IPR008333; OxRdtase_FAD-bd.
DR   InterPro; IPR001433; OxRdtase_FAD/NAD_bd.
DR   Pfam; PF00970; FAD_binding_6; 1.
DR   Pfam; PF00175; NAD_binding_1; 1.
DR   PRINTS; PR00406; CYTB5RDTASE.
DR   PRINTS; PR00371; FPNCR.
DR   PROSITE; PS51384; FAD_FR; 1.
PE   3: Inferred from homology;
KW   Complete proteome; FAD; Flavoprotein; Membrane; Mitochondrion;
KW   Mitochondrion outer membrane; NAD; Oxidoreductase; Transmembrane.
FT   CHAIN         1    296       NADH-cytochrome b5 reductase 2-A.
FT                                /FTId=PRO_0000330192.
FT   TRANSMEM     15     35       Potential.
FT   DOMAIN       47    151       FAD-binding FR-type.
FT   NP_BIND     154    189       FAD (By similarity).
SQ   SEQUENCE   296 AA;  33138 MW;  BF0E67ABEBB14308 CRC64;
     MFARIARINP KILPFVIGAP TIALCSYYYS SGAFLRNESS KVFIGDNNWI DLPISRIEEI
     SHDTKRFTFK YPSQDSVSGL VVASALLTKF VTPKGSNVIR PYTPVSDVDE KGSLDLVIKH
     YPDGKMTNHI FSLKVNDTLS FKGPIPKWKW VPNSFESITL IGGGTGITPL YQLIHAITKN
     PNDKTKIRLF YSNKTSQDVL MKKELDELQA KYPDQLRITY FITTPDKGYK GESGFISKEF
     IASNADKPSP KSHVFVCGPP PFMNAYSGDK KSPTDQGELV GILKELGYTI DQVYKF
//