A7THS1 (MCR1A_VANPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-cytochrome b5 reductase 2-A
EC=1.6.2.2

Alternative name(s):
Mitochondrial cytochrome b reductase A

Gene names

Name:	MCR1A
ORF Names:	Kpol_543p67

Organism

Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus) [Complete proteome]

Taxonomic identifier

436907 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Vanderwaltozyma

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May mediate the reduction of outer membrane cytochrome b5 By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	NADH-cytochrome b5 reductase 2-A		PRO_0000330192
Regions
	Transmembrane	15 – 35	21	Helical; Potential
	Domain	47 – 151	105	FAD-binding FR-type
	Nucleotide binding	154 – 189	36	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7THS1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: BF0E67ABEBB14308	FASTA	296	33,138
	10 20 30 40 50 60 MFARIARINP KILPFVIGAP TIALCSYYYS SGAFLRNESS KVFIGDNNWI DLPISRIEEI 70 80 90 100 110 120 SHDTKRFTFK YPSQDSVSGL VVASALLTKF VTPKGSNVIR PYTPVSDVDE KGSLDLVIKH 130 140 150 160 170 180 YPDGKMTNHI FSLKVNDTLS FKGPIPKWKW VPNSFESITL IGGGTGITPL YQLIHAITKN 190 200 210 220 230 240 PNDKTKIRLF YSNKTSQDVL MKKELDELQA KYPDQLRITY FITTPDKGYK GESGFISKEF 250 260 270 280 290 IASNADKPSP KSHVFVCGPP PFMNAYSGDK KSPTDQGELV GILKELGYTI DQVYKF

References

[1]

"Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication." Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H. Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed: 17494770] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 22028 / DSM 70294.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS480392 Genomic DNA. Translation: EDO18237.1.
RefSeq	XP_001646095.1. XM_001646045.1.
3D structure databases
ProteinModelPortal	A7THS1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5546512.
KEGG	vpo:Kpol_543p67.
Phylogenomic databases
OrthoDB	EOG4FFH9V.
PhylomeDB	A7THS1.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
KO	K00326.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCR1A_VANPO

Accession

Primary (citable) accession number: A7THS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	October 2, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program