Reviewed, UniProtKB/Swiss-Prot A7THG7 (LKHA4_VANPO)

Names and origin

Protein names

Recommended name:
    Leukotriene A-4 hydrolase
    EC=3.3.2.6
Alternative name(s):
    Leukotriene A(4) hydrolase
      Short name=LTA-4 hydrolase

Gene names

ORF Names:

Kpol_1039p41

Organism

Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus) [Complete proteome]

Taxonomic identifier

436907 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Vanderwaltozyma

Protein attributes

Sequence length	656 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes an epoxide moiety of LTA4 to form LTB4. The enzyme also has some peptidase activity By similarity.
Catalytic activity	(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O = (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Lipid metabolism; leukotriene B4 biosynthesis.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase M1 family.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Cellular component	Cytoplasm Nucleus
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	leukotriene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	leukotriene-A4 hydrolase activity Inferred from electronic annotation. Source: EC metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 656	656	Leukotriene A-4 hydrolase		PRO_0000324941
Sites
	Active site	332	1	By similarity
	Active site	420	1	Proton donor By similarity
	Metal binding	331	1	Zinc; catalytic By similarity
	Metal binding	335	1	Zinc; catalytic By similarity
	Metal binding	354	1	Zinc; catalytic By similarity
	Site	235	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7THG7-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 5768A5A9E11A4AFF	FASTA	656	74,538
	10 20 30 40 50 60 MLSQFTRSLS CFVKPTPPKL NKLTRTMTVL PKILQDARPK VSPELDYSTY SNYQNFKINH 70 80 90 100 110 120 TDLNLNISFD DSIVNGFVTF NLNKIDKSCN EIRLDTSFLT VDSVEIDDAK VDFQLKDRLE 130 140 150 160 170 180 PLGSQLIINP PTATSLNNEF NLKLGFSTTK DCTALQWLNG EQTSSGKPYV FSQLEAIHAR 190 200 210 220 230 240 ALFPCFDTPS VKSTFNAAIT STLPVVFSGI EQSVVDNGNG TKTYNFKQSV PIPAYLIGIA 250 260 270 280 290 300 SGDLVSGEIG PRSKVYTEPF RLKDCEWEFS GDVEKFIQAA EKIIFPYEWG TYDILVNVNS 310 320 330 340 350 360 YPYGGMESPN MTFATPTLIA YDKSNIDVIA HELAHSWSGN LVTNCSWNHF WLNEGWTVYL 370 380 390 400 410 420 ERRIVAAIHG EATRHFSALI GWNDLANSIS AMKNPDRFST LIQNLNDGTD PDEAFSSVPY 430 440 450 460 470 480 EKGFNLLFHL ETILGGPKEF DPFIKHYFTK FSKKSLDSYQ FFDTLFEFFA DKREILDAVD 490 500 510 520 530 540 WETWLYKPGM PPKPKFITTL ADNVYSLAEK WATEIKNGNT TEDDLKQAFT AADIKDFNSN 550 560 570 580 590 600 QIVLFLDTLV QNKEIQWNNH HTAAKTLLKI YEDSIVKSRN AEVVFRTYRF EITAQLKESY 610 620 630 640 650 PQLAEWLATV GRMKFVRPGY RLLNSVDRPL ALATFEKLQN IYHPICKALV KQDLEA

References

[1]

"Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication." Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H. Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed: 17494770] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DS480391 Genomic DNA. Translation: EDO18291.1.
RefSeq	XP_001646149.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5546571.
KEGG	vpo:Kpol_1039p41.
Family and domain databases
InterPro	IPR012777. Leuk_A4_hydro_aminopept. IPR006025. Pept_M_Zn_BS. IPR001930. Peptidase_M1. IPR015211. Peptidase_M1_C. IPR014782. Peptidase_M1_N. [Graphical view]
PANTHER	PTHR11533. Peptidase_M1. 1 hit.
Pfam	PF09127. Leuk-A4-hydro_C. 1 hit. PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
TIGRFAMs	TIGR02411. leuko_A4_hydro. 1 hit.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LKHA4_VANPO

Accession

Primary (citable) accession number: A7THG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	October 2, 2007
Last modified:	October 13, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)