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A7TGY3

- UBP4_VANPO

UniProt

A7TGY3 - UBP4_VANPO

Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene

DOA4

Organism
Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Required for promoting coordination of DNA replication and avoids DNA overreplication By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Enzyme regulationi

    RFU1 is an inhibitor of deubiquitination activity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei528 – 5281NucleophilePROSITE-ProRule annotation
    Active sitei836 – 8361Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 4
    Ubiquitin thioesterase 4
    Ubiquitin-specific-processing protease 4
    Gene namesi
    Name:DOA4
    Synonyms:UBP4
    ORF Names:Kpol_1032p28
    OrganismiVanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus)
    Taxonomic identifieri436907 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeVanderwaltozyma
    ProteomesiUP000000267: Unassembled WGS sequence

    Subcellular locationi

    Cytoplasm By similarity. Late endosome membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. late endosome membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 882882Ubiquitin carboxyl-terminal hydrolase 4PRO_0000376821Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi436907.A7TGY3.

    Structurei

    3D structure databases

    ProteinModelPortaliA7TGY3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini182 – 308127RhodanesePROSITE-ProRule annotationAdd
    BLAST
    Domaini519 – 879361USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 rhodanese domain.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5533.
    KOiK11839.
    OrthoDBiEOG7R2BSX.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR001763. Rhodanese-like_dom.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00581. Rhodanese. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view]
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7TGY3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVEVDSRKQL LYDPIVRLSG IADKFVMQDA TSSNMKVSLQ ECIDTLANYQ    50
    DECKKLKRNE PTLSPSERYS IYESAYIYYK IIHIMVLTRI PSLPQFSSAK 100
    SSDATNEDKE LMQIYNMLVK TLLSDEKIAQ IKSYLRANYP DKNSKGKESI 150
    VNKQLLNNEV FMLPLSGSPI SAVQLNHLIQ MYDSSLLLID VRPRAEFDSK 200
    HIKAKSVICV EPVSFKNSFT DLEVEKKSLI TSPQKEIALF QARDKYNYIV 250
    IYTQQSEKTQ FYMHQQLVLL DILMNKSFAK PLNEKNIKVF TLDKGFSGWV 300
    SKKGACETTT QNGDAIYISG NTSSLNLQNL PQLSPNIGSS MDKSMRDMMS 350
    TSADFEGRTY QLPQQQQPVF ARTPSFKNLF NKAKSSSTSS VTSSSPAPSQ 400
    LVRPQTSSMP PLEQNFTQYP ETPKLLTQIN TNTMPLSQIS PISSRAMSPM 450
    TKNMLTQSPQ LPMKISRTTI GNGAMLDLKP HPDSKPPGQP VPALPQLPHH 500
    MTGTYQNLNQ PKLDLDFTVG LENMGNSCYM NCIIQCLLST HELSQIFLNN 550
    SYEKHINLNS KLGSKGVLAK YFARLVHTMY REGSFKRPLE KNKPIQPIQF 600
    KMACGSINSL FKDNTQQDSQ EFCQFLLDGL HEDLNQCGAN PPLKELSEDA 650
    EKMREKLSMR IASSIEWERF LTTDFSVIVD LFQGQYASQL KCKVCGCTST 700
    TYQTFSVLSV PVPHVSSCHI LDCFNEFTKV EKLGTDELWS CPTCKKKQPS 750
    TKKLTITRLP RNLIIHLKRF DNMMNKNNVF VKYPFLLDLT PYWANDFDGR 800
    LPPGVTDELP TRGQVPPFRY KLNAVASHVG SLYGGHYTAY VNKGINRGWH 850
    YFDDTSYRPI KNETECITPN AYVLFYHRVY GV 882
    Length:882
    Mass (Da):100,061
    Last modified:October 2, 2007 - v1
    Checksum:i2C55FD7734FDE34A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS480389 Genomic DNA. Translation: EDO18435.1.
    RefSeqiXP_001646293.1. XM_001646243.1.

    Genome annotation databases

    GeneIDi5546722.
    KEGGivpo:Kpol_1032p28.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS480389 Genomic DNA. Translation: EDO18435.1 .
    RefSeqi XP_001646293.1. XM_001646243.1.

    3D structure databases

    ProteinModelPortali A7TGY3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 436907.A7TGY3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5546722.
    KEGGi vpo:Kpol_1032p28.

    Phylogenomic databases

    eggNOGi COG5533.
    KOi K11839.
    OrthoDBi EOG7R2BSX.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR001763. Rhodanese-like_dom.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00581. Rhodanese. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view ]
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication."
      Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H.
      Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 22028 / DSM 70294.

    Entry informationi

    Entry nameiUBP4_VANPO
    AccessioniPrimary (citable) accession number: A7TGY3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2009
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3