A7TGY3 (UBP4_VANPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 4
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4

Gene names

Name:	DOA4
Synonyms:	UBP4
ORF Names:	Kpol_1032p28

Organism

Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus) [Complete proteome]

Taxonomic identifier

436907 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Vanderwaltozyma › Vanderwaltozyma polyspora

Protein attributes

Sequence length	882 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Required for promoting coordination of DNA replication and avoids DNA overreplication By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Enzyme regulation	RFU1 is an inhibitor of deubiquitination activity By similarity.
Subcellular location	Cytoplasm By similarity. Late endosome membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the peptidase C19 family. Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Endosome Membrane
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	late endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 882	882	Ubiquitin carboxyl-terminal hydrolase 4		PRO_0000376821
Regions
	Domain	182 – 308	127	Rhodanese
Sites
	Active site	528	1	Nucleophile By similarity
	Active site	836	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7TGY3 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 2C55FD7734FDE34A	FASTA	882	100,061
	10 20 30 40 50 60 MVEVDSRKQL LYDPIVRLSG IADKFVMQDA TSSNMKVSLQ ECIDTLANYQ DECKKLKRNE 70 80 90 100 110 120 PTLSPSERYS IYESAYIYYK IIHIMVLTRI PSLPQFSSAK SSDATNEDKE LMQIYNMLVK 130 140 150 160 170 180 TLLSDEKIAQ IKSYLRANYP DKNSKGKESI VNKQLLNNEV FMLPLSGSPI SAVQLNHLIQ 190 200 210 220 230 240 MYDSSLLLID VRPRAEFDSK HIKAKSVICV EPVSFKNSFT DLEVEKKSLI TSPQKEIALF 250 260 270 280 290 300 QARDKYNYIV IYTQQSEKTQ FYMHQQLVLL DILMNKSFAK PLNEKNIKVF TLDKGFSGWV 310 320 330 340 350 360 SKKGACETTT QNGDAIYISG NTSSLNLQNL PQLSPNIGSS MDKSMRDMMS TSADFEGRTY 370 380 390 400 410 420 QLPQQQQPVF ARTPSFKNLF NKAKSSSTSS VTSSSPAPSQ LVRPQTSSMP PLEQNFTQYP 430 440 450 460 470 480 ETPKLLTQIN TNTMPLSQIS PISSRAMSPM TKNMLTQSPQ LPMKISRTTI GNGAMLDLKP 490 500 510 520 530 540 HPDSKPPGQP VPALPQLPHH MTGTYQNLNQ PKLDLDFTVG LENMGNSCYM NCIIQCLLST 550 560 570 580 590 600 HELSQIFLNN SYEKHINLNS KLGSKGVLAK YFARLVHTMY REGSFKRPLE KNKPIQPIQF 610 620 630 640 650 660 KMACGSINSL FKDNTQQDSQ EFCQFLLDGL HEDLNQCGAN PPLKELSEDA EKMREKLSMR 670 680 690 700 710 720 IASSIEWERF LTTDFSVIVD LFQGQYASQL KCKVCGCTST TYQTFSVLSV PVPHVSSCHI 730 740 750 760 770 780 LDCFNEFTKV EKLGTDELWS CPTCKKKQPS TKKLTITRLP RNLIIHLKRF DNMMNKNNVF 790 800 810 820 830 840 VKYPFLLDLT PYWANDFDGR LPPGVTDELP TRGQVPPFRY KLNAVASHVG SLYGGHYTAY 850 860 870 880 VNKGINRGWH YFDDTSYRPI KNETECITPN AYVLFYHRVY GV

References

[1]

"Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication." Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H. Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 22028 / DSM 70294.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS480389 Genomic DNA. Translation: EDO18435.1.
RefSeq	XP_001646293.1. XM_001646243.1.
3D structure databases
ProteinModelPortal	A7TGY3.
ModBase	Search...
Protein-protein interaction databases
STRING	436907.A7TGY3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5546722.
KEGG	vpo:Kpol_1032p28.
Phylogenomic databases
eggNOG	COG5533.
KO	K11839.
OrthoDB	EOG4HX88F.
Family and domain databases
Gene3D	3.40.250.10. 1 hit.
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR001763. Rhodanese-like_dom. [Graphical view]
Pfam	PF00581. Rhodanese. 1 hit. PF00443. UCH. 1 hit. [Graphical view]
SMART	SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP4_VANPO

Accession

Primary (citable) accession number: A7TGY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	October 2, 2007
Last modified:	April 3, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program