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A7TGY3

- UBP4_VANPO

UniProt

A7TGY3 - UBP4_VANPO

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Protein
Ubiquitin carboxyl-terminal hydrolase 4
Gene
DOA4, UBP4, Kpol_1032p28
Organism
Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Required for promoting coordination of DNA replication and avoids DNA overreplication By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

RFU1 is an inhibitor of deubiquitination activity By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei528 – 5281Nucleophile By similarity
Active sitei836 – 8361Proton acceptor By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. ubiquitinyl hydrolase activity Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene namesi
Name:DOA4
Synonyms:UBP4
ORF Names:Kpol_1032p28
OrganismiVanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus)
Taxonomic identifieri436907 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeVanderwaltozyma
ProteomesiUP000000267: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

  1. late endosome membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 882882Ubiquitin carboxyl-terminal hydrolase 4
PRO_0000376821Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi436907.A7TGY3.

Structurei

3D structure databases

ProteinModelPortaliA7TGY3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini182 – 308127Rhodanese
Add
BLAST
Domaini519 – 879361USP
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 1 rhodanese domain.
Contains 1 USP domain.

Phylogenomic databases

eggNOGiCOG5533.
KOiK11839.
OrthoDBiEOG7R2BSX.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7TGY3-1 [UniParc]FASTAAdd to Basket

« Hide

MVEVDSRKQL LYDPIVRLSG IADKFVMQDA TSSNMKVSLQ ECIDTLANYQ    50
DECKKLKRNE PTLSPSERYS IYESAYIYYK IIHIMVLTRI PSLPQFSSAK 100
SSDATNEDKE LMQIYNMLVK TLLSDEKIAQ IKSYLRANYP DKNSKGKESI 150
VNKQLLNNEV FMLPLSGSPI SAVQLNHLIQ MYDSSLLLID VRPRAEFDSK 200
HIKAKSVICV EPVSFKNSFT DLEVEKKSLI TSPQKEIALF QARDKYNYIV 250
IYTQQSEKTQ FYMHQQLVLL DILMNKSFAK PLNEKNIKVF TLDKGFSGWV 300
SKKGACETTT QNGDAIYISG NTSSLNLQNL PQLSPNIGSS MDKSMRDMMS 350
TSADFEGRTY QLPQQQQPVF ARTPSFKNLF NKAKSSSTSS VTSSSPAPSQ 400
LVRPQTSSMP PLEQNFTQYP ETPKLLTQIN TNTMPLSQIS PISSRAMSPM 450
TKNMLTQSPQ LPMKISRTTI GNGAMLDLKP HPDSKPPGQP VPALPQLPHH 500
MTGTYQNLNQ PKLDLDFTVG LENMGNSCYM NCIIQCLLST HELSQIFLNN 550
SYEKHINLNS KLGSKGVLAK YFARLVHTMY REGSFKRPLE KNKPIQPIQF 600
KMACGSINSL FKDNTQQDSQ EFCQFLLDGL HEDLNQCGAN PPLKELSEDA 650
EKMREKLSMR IASSIEWERF LTTDFSVIVD LFQGQYASQL KCKVCGCTST 700
TYQTFSVLSV PVPHVSSCHI LDCFNEFTKV EKLGTDELWS CPTCKKKQPS 750
TKKLTITRLP RNLIIHLKRF DNMMNKNNVF VKYPFLLDLT PYWANDFDGR 800
LPPGVTDELP TRGQVPPFRY KLNAVASHVG SLYGGHYTAY VNKGINRGWH 850
YFDDTSYRPI KNETECITPN AYVLFYHRVY GV 882
Length:882
Mass (Da):100,061
Last modified:October 2, 2007 - v1
Checksum:i2C55FD7734FDE34A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DS480389 Genomic DNA. Translation: EDO18435.1.
RefSeqiXP_001646293.1. XM_001646243.1.

Genome annotation databases

GeneIDi5546722.
KEGGivpo:Kpol_1032p28.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DS480389 Genomic DNA. Translation: EDO18435.1 .
RefSeqi XP_001646293.1. XM_001646243.1.

3D structure databases

ProteinModelPortali A7TGY3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 436907.A7TGY3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5546722.
KEGGi vpo:Kpol_1032p28.

Phylogenomic databases

eggNOGi COG5533.
KOi K11839.
OrthoDBi EOG7R2BSX.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view ]
SMARTi SM00450. RHOD. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication."
    Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H.
    Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 22028 / DSM 70294.

Entry informationi

Entry nameiUBP4_VANPO
AccessioniPrimary (citable) accession number: A7TGY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: October 2, 2007
Last modified: September 3, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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