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A7TGY3 (UBP4_VANPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 4

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene names
Name:DOA4
Synonyms:UBP4
ORF Names:Kpol_1032p28
OrganismVanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus) [Complete proteome]
Taxonomic identifier436907 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeVanderwaltozyma

Protein attributes

Sequence length882 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Required for promoting coordination of DNA replication and avoids DNA overreplication By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulation

RFU1 is an inhibitor of deubiquitination activity By similarity.

Subcellular location

Cytoplasm By similarity. Late endosome membrane; Peripheral membrane protein By similarity.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 rhodanese domain.

Contains 1 USP domain.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentCytoplasm
Endosome
Membrane
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentlate endosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 882882Ubiquitin carboxyl-terminal hydrolase 4
PRO_0000376821

Regions

Domain182 – 308127Rhodanese
Domain519 – 879361USP

Sites

Active site5281Nucleophile By similarity
Active site8361Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
A7TGY3 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: 2C55FD7734FDE34A

FASTA882100,061
        10         20         30         40         50         60 
MVEVDSRKQL LYDPIVRLSG IADKFVMQDA TSSNMKVSLQ ECIDTLANYQ DECKKLKRNE 

        70         80         90        100        110        120 
PTLSPSERYS IYESAYIYYK IIHIMVLTRI PSLPQFSSAK SSDATNEDKE LMQIYNMLVK 

       130        140        150        160        170        180 
TLLSDEKIAQ IKSYLRANYP DKNSKGKESI VNKQLLNNEV FMLPLSGSPI SAVQLNHLIQ 

       190        200        210        220        230        240 
MYDSSLLLID VRPRAEFDSK HIKAKSVICV EPVSFKNSFT DLEVEKKSLI TSPQKEIALF 

       250        260        270        280        290        300 
QARDKYNYIV IYTQQSEKTQ FYMHQQLVLL DILMNKSFAK PLNEKNIKVF TLDKGFSGWV 

       310        320        330        340        350        360 
SKKGACETTT QNGDAIYISG NTSSLNLQNL PQLSPNIGSS MDKSMRDMMS TSADFEGRTY 

       370        380        390        400        410        420 
QLPQQQQPVF ARTPSFKNLF NKAKSSSTSS VTSSSPAPSQ LVRPQTSSMP PLEQNFTQYP 

       430        440        450        460        470        480 
ETPKLLTQIN TNTMPLSQIS PISSRAMSPM TKNMLTQSPQ LPMKISRTTI GNGAMLDLKP 

       490        500        510        520        530        540 
HPDSKPPGQP VPALPQLPHH MTGTYQNLNQ PKLDLDFTVG LENMGNSCYM NCIIQCLLST 

       550        560        570        580        590        600 
HELSQIFLNN SYEKHINLNS KLGSKGVLAK YFARLVHTMY REGSFKRPLE KNKPIQPIQF 

       610        620        630        640        650        660 
KMACGSINSL FKDNTQQDSQ EFCQFLLDGL HEDLNQCGAN PPLKELSEDA EKMREKLSMR 

       670        680        690        700        710        720 
IASSIEWERF LTTDFSVIVD LFQGQYASQL KCKVCGCTST TYQTFSVLSV PVPHVSSCHI 

       730        740        750        760        770        780 
LDCFNEFTKV EKLGTDELWS CPTCKKKQPS TKKLTITRLP RNLIIHLKRF DNMMNKNNVF 

       790        800        810        820        830        840 
VKYPFLLDLT PYWANDFDGR LPPGVTDELP TRGQVPPFRY KLNAVASHVG SLYGGHYTAY 

       850        860        870        880 
VNKGINRGWH YFDDTSYRPI KNETECITPN AYVLFYHRVY GV 

« Hide

References

[1]"Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication."
Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H.
Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 22028 / DSM 70294.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS480389 Genomic DNA. Translation: EDO18435.1.
RefSeqXP_001646293.1. XM_001646243.1.

3D structure databases

ProteinModelPortalA7TGY3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING436907.A7TGY3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5546722.
KEGGvpo:Kpol_1032p28.

Phylogenomic databases

eggNOGCOG5533.
KOK11839.
OrthoDBEOG7R2BSX.

Family and domain databases

Gene3D3.40.250.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMSSF52821. SSF52821. 1 hit.
PROSITEPS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP4_VANPO
AccessionPrimary (citable) accession number: A7TGY3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: October 2, 2007
Last modified: April 16, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries