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A7TGY3

- UBP4_VANPO

UniProt

A7TGY3 - UBP4_VANPO

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Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene

DOA4

Organism
Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Required for promoting coordination of DNA replication and avoids DNA overreplication (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

RFU1 is an inhibitor of deubiquitination activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei528 – 5281NucleophilePROSITE-ProRule annotation
Active sitei836 – 8361Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. ubiquitinyl hydrolase activity Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene namesi
Name:DOA4
Synonyms:UBP4
ORF Names:Kpol_1032p28
OrganismiVanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus)
Taxonomic identifieri436907 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeVanderwaltozyma
ProteomesiUP000000267: Unassembled WGS sequence

Subcellular locationi

Cytoplasm By similarity. Late endosome membrane By similarity; Peripheral membrane protein By similarity

GO - Cellular componenti

  1. endosome Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 882882Ubiquitin carboxyl-terminal hydrolase 4PRO_0000376821Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi436907.A7TGY3.

Structurei

3D structure databases

ProteinModelPortaliA7TGY3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini182 – 308127RhodanesePROSITE-ProRule annotationAdd
BLAST
Domaini519 – 879361USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 rhodanese domain.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5533.
InParanoidiA7TGY3.
KOiK11839.
OrthoDBiEOG7R2BSX.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7TGY3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVEVDSRKQL LYDPIVRLSG IADKFVMQDA TSSNMKVSLQ ECIDTLANYQ
60 70 80 90 100
DECKKLKRNE PTLSPSERYS IYESAYIYYK IIHIMVLTRI PSLPQFSSAK
110 120 130 140 150
SSDATNEDKE LMQIYNMLVK TLLSDEKIAQ IKSYLRANYP DKNSKGKESI
160 170 180 190 200
VNKQLLNNEV FMLPLSGSPI SAVQLNHLIQ MYDSSLLLID VRPRAEFDSK
210 220 230 240 250
HIKAKSVICV EPVSFKNSFT DLEVEKKSLI TSPQKEIALF QARDKYNYIV
260 270 280 290 300
IYTQQSEKTQ FYMHQQLVLL DILMNKSFAK PLNEKNIKVF TLDKGFSGWV
310 320 330 340 350
SKKGACETTT QNGDAIYISG NTSSLNLQNL PQLSPNIGSS MDKSMRDMMS
360 370 380 390 400
TSADFEGRTY QLPQQQQPVF ARTPSFKNLF NKAKSSSTSS VTSSSPAPSQ
410 420 430 440 450
LVRPQTSSMP PLEQNFTQYP ETPKLLTQIN TNTMPLSQIS PISSRAMSPM
460 470 480 490 500
TKNMLTQSPQ LPMKISRTTI GNGAMLDLKP HPDSKPPGQP VPALPQLPHH
510 520 530 540 550
MTGTYQNLNQ PKLDLDFTVG LENMGNSCYM NCIIQCLLST HELSQIFLNN
560 570 580 590 600
SYEKHINLNS KLGSKGVLAK YFARLVHTMY REGSFKRPLE KNKPIQPIQF
610 620 630 640 650
KMACGSINSL FKDNTQQDSQ EFCQFLLDGL HEDLNQCGAN PPLKELSEDA
660 670 680 690 700
EKMREKLSMR IASSIEWERF LTTDFSVIVD LFQGQYASQL KCKVCGCTST
710 720 730 740 750
TYQTFSVLSV PVPHVSSCHI LDCFNEFTKV EKLGTDELWS CPTCKKKQPS
760 770 780 790 800
TKKLTITRLP RNLIIHLKRF DNMMNKNNVF VKYPFLLDLT PYWANDFDGR
810 820 830 840 850
LPPGVTDELP TRGQVPPFRY KLNAVASHVG SLYGGHYTAY VNKGINRGWH
860 870 880
YFDDTSYRPI KNETECITPN AYVLFYHRVY GV
Length:882
Mass (Da):100,061
Last modified:October 2, 2007 - v1
Checksum:i2C55FD7734FDE34A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS480389 Genomic DNA. Translation: EDO18435.1.
RefSeqiXP_001646293.1. XM_001646243.1.

Genome annotation databases

GeneIDi5546722.
KEGGivpo:Kpol_1032p28.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS480389 Genomic DNA. Translation: EDO18435.1 .
RefSeqi XP_001646293.1. XM_001646243.1.

3D structure databases

ProteinModelPortali A7TGY3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 436907.A7TGY3.

Protein family/group databases

MEROPSi C19.005.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5546722.
KEGGi vpo:Kpol_1032p28.

Phylogenomic databases

eggNOGi COG5533.
InParanoidi A7TGY3.
KOi K11839.
OrthoDBi EOG7R2BSX.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view ]
SMARTi SM00450. RHOD. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication."
    Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H.
    Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 22028 / DSM 70294.

Entry informationi

Entry nameiUBP4_VANPO
AccessioniPrimary (citable) accession number: A7TGY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: October 2, 2007
Last modified: October 29, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3