Reviewed, UniProtKB/Swiss-Prot A7TG13 (ATG15_VANPO)

Names and origin

Protein names

Recommended name:
    Putative lipase ATG15
    EC=3.1.1.3
Alternative name(s):
    Autophagy-related protein 15

Gene names

Name:	ATG15
ORF Names:	Kpol_1028p44

Organism

Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus) [Complete proteome]

Taxonomic identifier

436907 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Vanderwaltozyma

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Putative lipase ATG15		PRO_0000317973
Regions
	Topological domain	1 – 21	21	Cytoplasmic By similarity
	Transmembrane	22 – 42	21	Signal-anchor for type II membrane protein
	Topological domain	43 – 565	523	Lumenal By similarity
Sites
	Active site	347	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	217	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A7TG13-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 77BD72FDCD694A1F	FASTA	565	63,915
	10 20 30 40 50 60 MISNDYTKFS SKRRSLRYSN RILLLMGTIL LIVVYFYSDI LVDKSIIMFR NENKGQMLSG 70 80 90 100 110 120 SFTLRHIYRH GVDKGHRTQE VLDITKQNED AFGRYFKREL DEFNANAVGL NTDDPLRELW 130 140 150 160 170 180 TSDKQFITDN PFNFKFGLKG NKQILHRMVD RDPSFIEPYL DFARESPDMA AKVTIDWVEG 190 200 210 220 230 240 GEVVVVPDIT DKNTVVSLAL MSSNAYVRLP YTDDWRNVSL PWDSNDTPGY GWESNGIRGH 250 260 270 280 290 300 VFVNDLENIV VISIKGTSAQ GLPGSGEDET TGNDKLNDNL LFSCCCARVS YLWTTVCDCY 310 320 330 340 350 360 LKSYTCDETC LEQAIKEKDH YYQAAMDIYK DTLRQYPHAT IWLTGHSLGG ALASLVGRTY 370 380 390 400 410 420 GLPTVAFESP GELLAAKRLH LPFPPGLPSY DEGIWHIGHT ADPIYMGTCN GASSTCSIAG 430 440 450 460 470 480 YAMETGCHSG KQCVYDVVRD KGWHVNMLNH RIHTVIDGIL TKYDKVATCH EPDPCVDCYN 490 500 510 520 530 540 WNFMPHGKKP KKQTTSSSSE KVDTSTTKSI DRTTITTRTN EKKWHPNPKD PSTTTTDDKT 550 560 LMSSCLHRNW VGICTEYTTF TKRLI

References

[1]

"Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication." Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H. Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007) [PubMed: 17494770] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DS480385 Genomic DNA. Translation: EDO18770.1.
RefSeq	XP_001646628.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5547084.
KEGG	vpo:Kpol_1028p44.
Family and domain databases
InterPro	IPR002921. Lipase_3. IPR008262. Lipase_Ser_AS. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_VANPO

Accession

Primary (citable) accession number: A7TG13

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 2, 2007
Last modified:	October 13, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)